MEKK2 (MAP3K2) (NM_006609) Human Untagged Clone

CAT#: SC115980

MAP3K2 (untagged)-Human mitogen-activated protein kinase kinase kinase 2 (MAP3K2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEKK2
• Synonyms: MEKK2; MEKK2B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006609 edited
  ATGGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCC
  AGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAA
  CAGAATGATGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCC
  AGACCAGTTAAACTGGAAGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAGTCTATG
  GATCTACATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTGGAC
  AAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGATATTACTTGTA
  ATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGAT
  AATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGA
  GATAGAAGTTCTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAAT
  GGGTCATTCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATG
  CTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGTTGTCCATCACTT
  GATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAATGCCTAGGGCTCAGAGCTAC
  CCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAAATTTGGAAAA
  GGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGT
  CGTAAAACTTTTCCAAGAGCTAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGT
  TTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAG
  TATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTA
  ATGGACATCAGCCCACCCAGCCGTTCACCTCGAGCTCCGACCAACTGGAGATTGGGCAAA
  CTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGA
  GAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAAGTA
  AATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAG
  TATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATG
  CCAGGGGGTTCAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACT
  AGGAAATACACCCGTCAGATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTC
  CATAGAGATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTAGGA
  GATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGACAGGAATGAAGTCT
  GTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGGCTATGGAAGA
  AAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCT
  TGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCA
  AAGCTGCCACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAG
  GCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGTGCATTATCACTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006609 unedited
  CGCGTCAGATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGGCCTGA
  CGAGGGTGCCGCGGCCGCTGGGTGCAGCGTTCGGGGGTTGCACGGCCCGCGGCGGCGACG
  AAAACAACGACCAGGACGCTCGGCGCCCCTGTCCGCAGAGTCGCCACTCCCGCCTCTTCT
  CCGGTCTCTAGCGACGCGGGCCCGCGGCTTCCTCCTTTCCTGCCCAGTTCCCGCGGGCCT
  CGGGGTTGCAGCGGCTAGAAGCGCCGAGGAGCGCCAGGAGGACCCGGCCGGCTGCGAGGA
  TTCACGCGCCCGCCACCCCGCCCGGCCCGGGCTTCATGTGAAAGAGGGACGGGCCTGGCC
  CCAAGGCCCGCGCCCGACTCCTCGCGCGCCCCTCCGGTCCCCGGGGGCCCTGCGGCAGCC
  CCCGACCTGGGGCCGCGGTGACAGGCCGCGCTGGGGCGGAGGGGCGGGCTCGGCGGGGGC
  GGCCCCTCCTTGGGCCTGCGGCTCTCTACGTCCCTGCGGAGCAGCCGGCCATAAAGAATG
  CTGATGGGAGAACCATTTTCCTAATTTTCAAATTGTTGAGCTGTTTGCCATAATGGATGA
  TCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACC
  AGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGA
  TGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGT
  TAAACTGGAAGATCTGAGATCTAAAGCTAANNATGCCTTTGGACAGTCTATGGATCTACA
  TTATACCAATAACGAGTTGGGTAATCCATTAACTACTCAAGATGACTGGNACAAAGCTGT
  GGAACTGCTGGATCGTAGTATTCATATGAAGAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006609 unedited
  TGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGCCCAAATCATGA
  ATTTTAAGGAAAGAATATATAGAGATGGCTGAGTTCAAAAGGCTCAGTGTTCAAATTCAG
  TTTGTTCCTAAAATGAAGAGTTACCTATGTGGGTGCAATATGCAGCTGGTAAAGTGATTG
  CTATTTGCTGTTTGTTGAGATTATTCACCCTTGACTTAAAGCAGCAGTATCTGATCTTGT
  AAAATCCTCAATTTGCATTACATCACTTTCTCTTTGCGACTTCCTTTTCTTTCTTGCATT
  TACTGCTTTGTAAATAGCTGTTTTCAGTTTATAACTGGGACTGATCTTTACATCAGGGTT
  TCTCAGCCTCAGCACTTCTGACATTTTGGGAGGGGTAATTCTTTGAGGCTGCTTTCCTTG
  TGTATTATAATCTATTTAGCAACATCCCTGGCCTCTACCCAATTCATGCTACTAGTATCC
  CTCCAATTGTGACAACCGGAAATGTCTCTAAGAATTGCCAAATGCCTAGTGAAATCATCC
  TCGCTCCACTTTTGGCAACCACTGTTTCACATGATACCTGTTTTTTTGAGGAGCTTTAGT
  ATTCTGTGATCCTAAGAACAAGGGTTTCATCTCCTGACATAACACATAAACATTACTAAT
  TGAACTCTTCGCTCCTAAGGGATGTTACCTATGGGGAATCAGGAGCTGGAAATAGAAGAT
  GGTATACATGATTCTGATTATTTCTNCATTCCTTTAATTCTGGGACGTNCCCCTCAAGTA
  AAACAAAACAAAACTGTTTAAAATACACTAAACACCTAATTTTTCTTCATTATTTCTATT
  GACTGGTAATGAGGNCTCCNCTACTGCTCAACATTTGATAAGATGATGNCCTTTTGTCTC
  TATTTCTATTTGCTCACACTTGACTTTAAATAAGGCTCTNCGCGNGTGGTGGTGAGGGGG
  GGTTTTTAGCAGAAGCTTCCCTCAGCTTACGATCCAGTACGGGATCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006609
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006609.2, NP_006600.2
• RefSeq Size: 3336 bp
• RefSeq ORF: 1863 bp
• Locus ID: 10746
• UniProt ID: Q9Y2U5
• Cytogenetics: 2q14.3
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]