MEKK2 (MAP3K2) (NM_006609) Human Tagged ORF Clone

CAT#: RC223988

MAP3K2 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 2 (MAP3K2)

Product Images

Circular map for RC223988

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  Western blot validation of overexpression lysate (Cat# LY416533) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223988 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAP3K2 protein (Cat# TP323988). The protein was produced from HEK293T cells transfected with MAP3K2 cDNA clone (Cat# RC223988) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MEKK2
• Synonyms: MEKK2; MEKK2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223988 representing NM_006609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACCAG
  CATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGATGTCCGAGTCAA
  ATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGTTAAACTGGAAGATCTGAGATCT
  AAAGCTAAAATTGCCTTTGGACAGTCTATGGATCTACATTATACCAATAACGAGTTGGTAATTCCATTAA
  CTACTCAAGATGACTTGGACAAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGAT
  ATTACTTGTAATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGAT
  AATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGAGATAGAAGTT
  CTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAATGGGTCATTCACTAGTATCAA
  CAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATGCTGGATCCATTATCTTTAAGCAGCCCTGAA
  AATTCTGGCTCAGGAAGTTGTCCATCACTTGATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAA
  TGCCTAGGGCTCAGAGCTACCCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAA
  ATTTGGAAAAGGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGT
  CGTAAAACTTTTCCAAGAGCTAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGTTTCAGTCCTA
  CTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAGTATGATGATAGTCGAATAAG
  AAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTAATGGACATCAGCCCACCCAGCCGTTCACCT
  CGAGCTCCGACCAACTGGAGATTGGGCAAACTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTT
  ATGATGTTGATACAGGAAGAGAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAG
  CAAGGAAGTAAATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAG
  TATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATGCCAGGGGGTT
  CAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACTAGGAAATACACCCGTCAGAT
  TCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTCCATAGAGATATCAAAGGCGCAAATATCCTG
  CGAGATTCAACAGGCAACGTCAAACTAGGAGATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCT
  CAGGGACAGGAATGAAGTCTGTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGG
  CTATGGAAGAAAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCT
  TGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCAAAGCTGCCAC
  CTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGC
  TGATGAACTCTTAAGGCACATGTTTGTGCATTATCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223988 representing NM_006609
  Red=Cloning site Green=Tags(s)
  
  MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRS
  KAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLD
  NTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPE
  NSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDG
  RKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSP
  RAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQ
  YYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANIL
  RDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPP
  WAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006609
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_006609.5
• RefSeq Size: 3336 bp
• RefSeq ORF: 1860 bp
• Locus ID: 10746
• UniProt ID: Q9Y2U5
• Cytogenetics: 2q14.3
• Domains: PB1, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway
• MW: 69.6 kDa
• Gene Summary: The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]