CysLT1 (CYSLTR1) (NM_006639) Human Untagged Clone

CAT#: SC115965

CYSLTR1 (untagged)-Human cysteinyl leukotriene receptor 1 (CYSLTR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CysLT1
• Synonyms: CYSLT1; CYSLT1R; CYSLTR; HMTMF81
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115965 sequence for NM_006639 edited (data generated by NextGen Sequencing)
  ATGGATGAAACAGGAAATCTGACAGTATCTTCTGCCACATGCCATGACACTATTGATGAC
  TTCCGCAATCAAGTGTATTCCACCTTGTACTCTATGATCTCTGTTGTAGGCTTCTTTGGC
  AATGGCTTTGTGCTCTATGTCCTCATAAAAACCTATCACAAGAAGTCAGCCTTCCAAGTA
  TACATGATTAATTTAGCAGTAGCAGATCTACTTTGTGTGTGCACACTGCCTCTCCGTGTG
  GTCTATTATGTTCACAAAGGCATTTGGCTCTTTGGTGACTTCTTGTGCCGCCTCAGCACC
  TATGCTTTGTATGTCAACCTCTATTGTAGCATCTTCTTTATGACAGCCATGAGCTTTTTC
  CGGTGCATTGCAATTGTTTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAAAAAGCC
  AGGTTTGTGTGTGTAGGTATTTGGATTTTTGTGATTTTGACCAGTTCTCCATTTCTAATG
  GCCAAACCACAAAAAGATGAGAAAAATAATACCAAGTGCTTTGAGCCCCCACAAGACAAT
  CAAACTAAAAATCATGTTTTGGTCTTGCATTATGTGTCATTGTTTGTTGGCTTTATCATC
  CCTTTTGTTATTATAATTGTCTGTTACACAATGATCATTTTGACCTTACTAAAAAAATCA
  ATGAAAAAAAATCTGTCAAGTCATAAAAAGGCTATAGGAATGATCATGGTCGTGACCGCT
  GCCTTTTTAGTCAGTTTCATGCCATATCATATTCAACGTACCATTCACCTTCATTTTTTA
  CACAATGAAACTAAACCCTGTGATTCTGTCCTTAGAATGCAGAAGTCCGTGGTCATAACC
  TTGTCTCTGGCTGCATCCAATTGTTGCTTTGACCCTCTCCTATATTTCTTTTCTGGGGGT
  AACTTTAGGAAAAGGCTGTCTACATTTAGAAAGCATTCTTTGTCCAGCGTGACTTATGTA
  CCCAGAAAGAAGGCCTCTTTGCCAGAAAAAGGAGAAGAAATATGTAAAGTATAG
  
  Clone variation with respect to NM_006639.2
  927 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006639 unedited
  NGGTTGAGGATTTTGTNATACGATTTACTATAGGGTCGGCCGCGAAATTCGCACGAGGTG
  CTTTTACGACCCTACAGGGTATCAAGATACTGTGCAGCTCGCCAACAAGGATTAATTGCA
  AGGACTGGTAGATCGAATTTACTGAAGACTTGGAGCTTGCTTCTGAGAACAAACGCAAAA
  GGACAGTAAACTGTGGACCTTGAAGTTAGCAGCGTGGGCTTCCTCTAATATTACACCGTA
  AAAGGCATTGATCACCATAAGAAGGAACATTTGTGAAGGTACTCCAGTGCCAGAAAGAGG
  CACAAAGCAGACATATGTAGAGAAACATGGATGAAACAGGAAATCTGACAGTATCTTCTA
  TCACATGCCATGACACTATTGATGACTTCCGCAATCAAGTGTATTCCACCTTGTACTCTA
  TGATCTCTGTTGTAGGCTTCTTTGGCAATGGCTTTGTGCTCTATGTCCTCATAAAAACCT
  ATCACAAGATAGTCAGCCTTCCAAGTATACATGATTAATTTAGCAGTAGCAGATCTACTT
  TGTGTGTGCACACTGCCTCTCCGTGTGGTCTATTATGTTCACAAAGGCATTTGGCTCTTT
  GGTGACTTCTTGTGCCGCCTCAGCACCTATGCTTTGTATGTCAACCTCTATTGTAGCATC
  TTCTTTATGACAGCCATGAGCTCTTTCCGGTGCATTGCAATTGTTTTTCCAGTCCAGAAC
  ATTAATTTGGTTACACAGAAAAAAAGCCAGGTTTGTGTGTGTAGGTATTTTGCATTTTTG
  TGATTTTGACCAGTTCTTCATTTCTAATGGCCACACCACCAAAAGATGATGAAAATAATA
  CCAAGTGCCTTTGAGCTCCCACAAGACATCAAACCTAAAAATCATGTTCTGGTCTCGCCA
  TATGTGGCATTGTTTGGTTGCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006639 unedited
  AAATAATAAANNNNNNNTTATTANNNNNGGCCACTTTTGATTACATTTTTTTTTTTGNAG
  TAAGTTACTTTTTAAAAACAACTTAAAGGCACACTACACTTCAGCAAAAGCTTGTCCACG
  CATATTATATTTCAGCCGTAGATGAGTTGTTGTGGCTGTTTTAAGATCAAAGCGTCCACT
  GTAATATAAAAGTCACTGTAAACATATCCATGATATTCCTGTCAACATACCAATAAAAAG
  TAATAAAGCAATAATAAAACTTATCGTGAACTTTCACCCATAATGCATATCATACAGCAA
  AATCTCTTACATATAGTTGTATACATATACATAAATGTATGCACTTCTTAAAAGAATTTG
  TAACGTGAACATTCACAATTTTCTTCATAAATCCTTTGTAGTTTATTTTGATCCCAGCAA
  TTGGCATATAGAAAAGTAGATTCCTTTTCCTTTTCTCTTCTTCAGATAAGTTTTTAATTA
  CTTCTATTTATATATAGGAGTGCCAGTTGTCTGGCTTCTTAAGGAAGAATTGTATGTCAT
  GGATGGAGAGAAATCTCTACAGGAACCTCACTGAGCAGCTAAAACTCCACTATTCAATTT
  TGGGAGCCTGAGGCAAAGGCAGAAGAAAACTGGAGACAAACTGGCTTTTGACCAGATTTC
  TCCTGTTGCCATATTGAACATTGTATGGTGCTTTTAAGACCCTTTAGCTGAATACTAGTC
  TGTTCTAGCACTTTTACATTCTCTTTTGGAAAGAATGGCCCTTATTTGCCTAGCGAATCA
  GTACTACAAAAGAAGGTGACAGTACGTTTTATGTAACTCCTGAGTATCTTCAGTTCTCAT
  CTGATGGCATGCATGCAAAGGAATATGTAGAATGATTCAGAACTATTTGGCCATTTATAT
  CTAGTTTTATTTTTAATACTTTAACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006639
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006639.2, NP_006630.1
• RefSeq Size: 1537 bp
• RefSeq ORF: 1014 bp
• Locus ID: 10800
• UniProt ID: Q9Y271
• Cytogenetics: Xq21.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.