B4GAT1 (NM_006876) Human Untagged Clone

CAT#: SC115826

B3GNT1 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B4GAT1
• Synonyms: B3GN-T1; B3GNT1; B3GNT6; BETA3GNTI; iGAT; iGNT; MDDGA13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115826 sequence for NM_006876 edited (data generated by NextGen Sequencing)
  ATGCAGATGTCCTACGCCATCCGGTGCGCCTTCTACCAGCTGCTGCTGGCCGCGCTCATG
  CTGGTGGCGATGCTGCAGCTGCTCTACCTGTCGCTGCTGTCCGGACTGCACGGGCAGGAG
  GAGCAAGACCAATATTTTGAGTTCTTTCCCCCGTCCCCACGGTCCGTGGACCAGGTCAAG
  GCGCAGCTCCGCACCGCGCTGGCCTCTGGAGGCGTCCTGGACGCTAGCGGCGATTACCGC
  GTCTACAGGGGCCTGCTGAAGACCACCATGGACCCCAACGATGTGATCCTGGCCACGCAC
  GCCAGCGTGGACAACCTGCTGCACCTGTCGGGTCTGCTGGAGCGCTGGGAGGGCCCGCTG
  TCCGTGTCGGTGTTCGCGGCCACCAAGGAGGAGGCGCAGCTGGCCACGGTGCTGGCCTAC
  GCGCTGAGCAGCCACTGCCCCGACATGCGCGCCAGGGTCGCCATGCACCTCGTGTGCCCC
  TCGCGTTACGAGGCAGCCGTGCCCGACCCCCGGGAGCCGGGGGAGTTTGCCCTGCTGCGG
  TCCTGCCAGGAGGTCTTTGACAAGCTAGCCAGGGTGGCCCAGCCCGGGATTAATTATGCG
  CTGGGCACCAATGTCTCCTACCCCAATAACCTGCTGAGGAATCTGGCTCGTGAGGGGGCC
  AACTATGCCCTGGTGATCGATGTGGACATGGTGCCCAGCGAGGGGCTGTGGAGAGGCCTG
  CGGGAAATGCTGGATCAGAGCAACCAGTGGGGAGGCACCGCGCTGGTGGTGCCTGCCTTC
  GAAATCCGAAGAGCCCGCCGCATGCCCATGAACAAAAACGAGCTGGTGCAGCTCTACCAG
  GTTGGCGAGGTGCGGCCCTTCTATTATGGGTTGTGCACCCCCTGCCAGGCACCCACCAAC
  TATTCCCGCTGGGTCAACCTGCCGGAAGAGAGCTTGCTGCGGCCCGCCTACGTGGTACCT
  TGGCAGGACCCCTGGGAGCCATTCTACGTGGCAGGAGGCAAGGTGCCCACCTTCGACGAG
  CGCTTTCGGCAGTACGGCTTCAACCGAATCAGCCAGGCCTGCGAGCTGCATGTGGCGGGG
  TTTGATTTTGAGGTCCTGAACGAAGGTTTCTTGGTTCATAAGGGCTTCAAAGAAGCGTTG
  AAGTTCCATCCCCAAAAGGAGGCTGAAAATCAGCACAATAAGATCCTATATCGCCAGTTC
  AAACAGGAGTTGAAGGCCAAGTACCCCAACTCTCCCCGACGCTGCTGA
  
  Clone variation with respect to NM_006876.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006876 unedited
  NNGGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTAAAT
  CCGGGCTTGCGGCCGCTGGCGTAGTCTGTGGCCGGGTGGTCGTTGCTGCGCGCCCCGAGC
  CCCGAGAGCCATGCAGATGTCCTACGCCATCCGGTGCGCCTTCTACCAGCTGCTGCTGGC
  CGCGCTCATGCTGGTGGCGATGCTGCAGCTGCTCTACCTGTCGCTGCTGTCCGGACTGCA
  CGGGCAGGAGGAGCAAGACCAATATTTTGAGTTCTTTCCCCCGTCCCCACGGTCCGTGGA
  CCAGGTCAAGGCGCAGCTCCGCACCGCGCTGGCCTCTGGAGGCGTCCTGGACGCTAGCGG
  CGATTACCGCGTCTACAGGGGCCTGCTGAAGACCACCATGGACCCCAACGATGTGATCCT
  GGCCACGCACGCCAGCGTGGACAACCTGCTGCACCTGTCGGGTCTGCTGGAGCGCTGGGA
  GGGCCCGCTGTCCGTGTCGGTGTTCGCGGCCACCAAGGAGGAGGCGCAGCTGGCCACGGT
  GCTGGCCTACGCGCTGAGCAGCCACTGCCCCGACATGCGCGCCAGGGTCGCCATGCACCT
  CGTGTGCCCCTCGCGTTACGAGGCAGCCGTGCCCGACCCCCGGGAGCCGGGGGAGTTTGC
  CCTGCTGCGGTCCTGCCAGGAGGTCTTTGACAAGCTAGCCAGGGTGGCCCAGCCCGGGAT
  TAATTATGCGCTGGGCACCAATGTCTCCTACCCCAATAACCTGCTGAGGAATCTGGCTCG
  NTGAGGGGGCCAACTATGCCCCTGGTGATCGATGTGGACATGGTGCCCANCGAGGGGGGC
  TGTTGGAAAGGCCTGCNGGAAATGCTGGNATCANAGCACAGTGGGGGGGAGCACCGGCCT
  CTGGTGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006876 unedited
  TGGACCGCGGCACGCATNCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTGGGTGTGAACA
  CGCATTTATTTACACATTGTCATCGGTAGGCACATACCCAGCCCAGCCCGTTGAAGGGAC
  AGATCTCAGGCTGTCACCCATGGAACCTGCAGACCCTTCCCTCCTCCAGAACCCTTGAAG
  GCGAAGCCCTGAGAGAACCAGGAATTTTAGGCTTCTGTTCAAGAGCTAAGAACTAAATTT
  TATGCCTTCATCTGATTTCTTTCCAAAAAGTCCATTTCATTAAGTATTCAGACTTCTTAG
  CTCCATCCCATTCATACTTTTTGCTCTCCTACTACCCACCCAAGATTGGTAATAATAACA
  ATAATAATAACAACAATAATACTGCGATAATATTAATACTTCACATTTGTACGAAGCTTA
  CAGAATGGTTTCACATATAGCATCTCATCTGAGCCTCCCAACAGTTCCGTGAGGTAGGTA
  TTCTCACCTCCCTTTTTACAGACAGGGTAACCGAGGCTCAGAGAGGTAACGGGATTTACT
  CAAGGCCACACAGCTAGTTAGTGGTAAAGCTAGGAATCGATCCCAGCACCCCATATCCAA
  GTCCAGTGTTTCAACACCACAGCTACCTCTGTAAAGTGGAGCGACATTTCTTACCCCAGT
  CAGGCCTAAATGGTGGCCCTGAGAGCCAAGAAGCTGACTTCTCACATTACGGGAGGGCAA
  GGGCTCAACAGCGCCCGGGAAAGATGGGGTTCCTGGCCCTCCACTCCTGTGTGAACCGCC
  GCACTAGATCCTCTCGTGCCGATTTTACCCTCTCTGGGGTACGGAACCTCCAGGTTTTTT
  TATACCCTCATGACCCACAACCCTTGCTCCCGACCCCCAAATCACACCGCCCACTCCCCC
  CCAAGGCTGGTTATTTTGTCGTAAACCGTGTTTTAATTCTTTCTTCACCCGCCCCTGTTT
  TCCGCCTACAATCTCCCCGGTCCTTTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006876
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006876.1, NP_006867.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 1248 bp
• Locus ID: 11041
• UniProt ID: O43505
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. [provided by RefSeq, Jul 2008]