B4GAT1 (NM_006876) Human Tagged ORF Clone

CAT#: RC200256

B3GNT1 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1)

Product Images

Circular map for RC200256

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  Western blot validation of overexpression lysate (Cat# LY416356) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200256 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified B4GAT1 protein (Cat# TP300256). The protein was produced from HEK293T cells transfected with B4GAT1 cDNA clone (Cat# RC200256) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4GAT1
• Synonyms: B3GN-T1; B3GNT1; B3GNT6; BETA3GNTI; iGAT; iGNT; MDDGA13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200256 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGTCCTACGCCATCCGGTGCGCCTTCTACCAGCTGCTGCTGGCCGCGCTCATGCTGGTGGCGA
  TGCTGCAGCTGCTCTACCTGTCGCTGCTGTCCGGACTGCACGGGCAGGAGGAGCAAGACCAATATTTTGA
  GTTCTTTCCCCCGTCCCCACGGTCCGTGGACCAGGTCAAGGCGCAGCTCCGCACCGCGCTGGCCTCTGGA
  GGCGTCCTGGACGCTAGCGGCGATTACCGCGTCTACAGGGGCCTGCTGAAGACCACCATGGACCCCAACG
  ATGTGATCCTGGCCACGCACGCCAGCGTGGACAACCTGCTGCACCTGTCGGGTCTGCTGGAGCGCTGGGA
  GGGCCCGCTGTCCGTGTCGGTGTTCGCGGCCACCAAGGAGGAGGCGCAGCTGGCCACGGTGCTGGCCTAC
  GCGCTGAGCAGCCACTGCCCCGACATGCGCGCCAGGGTCGCCATGCACCTCGTGTGCCCCTCGCGTTACG
  AGGCAGCCGTGCCCGACCCCCGGGAGCCGGGGGAGTTTGCCCTGCTGCGGTCCTGCCAGGAGGTCTTTGA
  CAAGCTAGCCAGGGTGGCCCAGCCCGGGATTAATTATGCGCTGGGCACCAATGTCTCCTACCCCAATAAC
  CTGCTGAGGAATCTGGCTCGTGAGGGGGCCAACTATGCCCTGGTGATCGATGTGGACATGGTGCCCAGCG
  AGGGGCTGTGGAGAGGCCTGCGGGAAATGCTGGATCAGAGCAACCAGTGGGGAGGCACCGCGCTGGTGGT
  GCCTGCCTTCGAAATCCGAAGAGCCCGCCGCATGCCCATGAACAAAAACGAGCTGGTGCAGCTCTACCAG
  GTTGGCGAGGTGCGGCCCTTCTATTATGGGTTGTGCACCCCCTGCCAGGCACCCACCAACTATTCCCGCT
  GGGTCAACCTGCCGGAAGAGAGCTTGCTGCGGCCCGCCTACGTGGTACCTTGGCAGGACCCCTGGGAGCC
  ATTCTACGTGGCAGGAGGCAAGGTGCCCACCTTCGACGAGCGCTTTCGGCAGTACGGCTTCAACCGAATC
  AGCCAGGCCTGCGAGCTGCATGTGGCGGGGTTTGATTTTGAGGTCCTGAACGAAGGTTTCTTGGTTCATA
  AGGGCTTCAAAGAAGCGTTGAAGTTCCATCCCCAAAAGGAGGCTGAAAATCAGCACAATAAGATCCTATA
  TCGCCAGTTCAAACAGGAGTTGAAGGCCAAGTACCCCAACTCTCCCCGACGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200256 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQMSYAIRCAFYQLLLAALMLVAMLQLLYLSLLSGLHGQEEQDQYFEFFPPSPRSVDQVKAQLRTALASG
  GVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAY
  ALSSHCPDMRARVAMHLVCPSRYEAAVPDPREPGEFALLRSCQEVFDKLARVAQPGINYALGTNVSYPNN
  LLRNLAREGANYALVIDVDMVPSEGLWRGLREMLDQSNQWGGTALVVPAFEIRRARRMPMNKNELVQLYQ
  VGEVRPFYYGLCTPCQAPTNYSRWVNLPEESLLRPAYVVPWQDPWEPFYVAGGKVPTFDERFRQYGFNRI
  SQACELHVAGFDFEVLNEGFLVHKGFKEALKFHPQKEAENQHNKILYRQFKQELKAKYPNSPRRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006876
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006876.3
• RefSeq Size: 2077 bp
• RefSeq ORF: 1248 bp
• Locus ID: 11041
• UniProt ID: O43505
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
• MW: 47.1 kDa
• Gene Summary: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. [provided by RefSeq, Jul 2008]