RP2 (NM_006915) Human Untagged Clone

CAT#: SC115781

RP2 (untagged)-Human retinitis pigmentosa 2 (X-linked recessive) (RP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RP2
• Synonyms: DELXp11.3; NM23-H10; NME10; TBCCD2; XRP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115781 sequence for NM_006915 edited (data generated by NextGen Sequencing)
  ATGGGCTGCTTCTTCTCCAAGAGACGGAAGGCTGACAAGGAGTCGCGGCCCGAGAACGAG
  GAGGAGCGGCCAAAGCAGTACAGCTGGGATCAGCGCGAGAAGGTTGATCCAAAAGACTAC
  ATGTTCAGTGGACTGAAGGATGAAACAGTAGGTCGCTTACCTGGGACGGTAGCAGGACAA
  CAGTTTCTCATTCAAGACTGTGAGAACTGTAACATCTATATTTTTGATCACTCTGCTACA
  GTTACCATTGATGACTGTACTAACTGCATAATTTTTCTGGGACCCGTGAAAGGCAGCGTG
  TTTTTCCGGAATTGCAGAGATTGCAAGTGCACATTAGCCTGCCAACAATTTCGTGTGCGA
  GATTGTAGAAAGCTGGAAGTCTTTTTGTGTTGTGCCACTCAACCCATCATTGAGTCTTCC
  TCAAATATCAAATTTGGATGTTTTCAATGGTACTATCCTGAATTAGCTTTCCAGTTCAAA
  GATGCAGGGCTAAGTATCTTCAACAATACATGGAGTAACATTCATGACTTTACACCTGTG
  TCAGGAGAACTCAACTGGAGCCTTCTTCCAGAAGATGCTGTGGTTCAGGACTATGTTCCT
  ATACCTACTACCGAAGAGCTCAAAGCTGTTCGTGTTTCCACAGAAGCCAATAGAAGCATT
  GTTCCAATATCCCGGGGTCAGAGACAGAAGAGCAGCGATGAATCATGCTTAGTGGTATTA
  TTTGCTGGTGATTACACTATTGCAAATGCCAGAAAACTAATTGATGAGATGGTTGGTAAA
  GGCTTTTTCCTAGTTCAGACAAAGGAAGTGTCCATGAAAGCTGAGGATGCTCAAAGGGTT
  TTTCGGGAAAAAGCACCTGACTTCCTTCCTCTTCTGAACAAAGGTCCTGTTATTGCCTTG
  GAGTTTAATGGGGATGGTGCTGTAGAAGTATGTCAACTTATTGTAAACGAGATATTCAAT
  GGGACCAAGATGTTTGTATCTGAAAGCAAGGAGACGGCATCTGGAGATGTAGACAGCTTC
  TACAACTTTGCTGATATACAGATGGGAATATGA
  
  Clone variation with respect to NM_006915.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006915 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCCGATTCGGCACGAGGGGAAGTGCCTG
  AGCTAGTGAGCTGGCCAACGAGCTCCGCGGGCTGGGACCATGGGCTGCTTCTTCTCCAAG
  AGACGGAAGGCTGACAAGGAGTCGCGGCCCGAGAACGAGGAGGAGCGGCCAAAGCAGTAC
  AGCTGGGATCAGCGCGAGAAGGTTGATCCAAAAGACTACATGTTCAGTGGACTGAAGGAT
  GAAACAGTAGGTCGCTTACCTGGGACGGTAGCAGGACAACAGTTTCTCATTCAAGACTGT
  GAGAACTGTAACATCTATATTTTTGATCACTCTGCTACAGTTACCATTGATGACTGTACT
  AACTGCATAATTTTTCTGGGACCCGTGAAAGGCAGCGTGTTTTTCCGGAATTGCAGAGAT
  TGCAAGTGCACATTAGCCTGCCAACAATTTCGTGTGCGAGATTGTACAAAGCTGGAAGTC
  TTTTTGTGTTGTGCCACTCAACCCATCCATTGAGTCTTCCTCAAATATCAAATTTGGATG
  TTTTCAATGGTACTATCCTGAATTAGCTTTCCAGTTCAAAGATGCAGGGCTAAGTATCTT
  CAACAATACATGGAGTAACATTCATGACTTTACACCTTGTGTATGAGAACTCACCTGGAG
  CCCTCTTCCCGAAGATGCTGTGGCTCACGACTATGCTCCTATACCTACTACCGAAGAGCT
  CAAAGCTGTTCGTGTTTCCACAGAACCAATAAGAGCATTGTTCCATTTCCCCGGGTCAGA
  GACCGAACAGCACCACGAATCATGCTTAATGGCATTATTTCCTGTGATTACCCTATTGCA
  ACGCCACAAAACTAACTGAGAAACGGTCGGTAAGCCTTTTCCTATTAAAACAAGGACCGT
  TCTTGAAGCCCAGTGCTCACAGGTTTTCCGACAAAAACTGACTTCTTTCTTTTGAAACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006915 unedited
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTAACAACATTTATTACAAATCA
  CATTTTATAAACACTAGATAAATTACATATAAAAGCTAGACATAAATATAAAGAAGCAAA
  ACAAAAACAGCAGTACTATTAATGCAAGAGAAATAAGCTAATATAATATTTAAACACATA
  TAACTTAATAAAATTACCAATTTTTCATTGTTAATAATTTTTCATAAATTCAAAAGCTAT
  TCAGAAATGAAATGTAATACATTCTGATTACTATCAAAGGTTAGAATTATAGAAACAACC
  ATAGCACTACTTATTTTTTAAAATCGTTTCAAACATTCAGAAGCCAAATGACTGTCCTTA
  AAGACAAATTAAATGGGTAAAAGGTCTTATCTAACAGAAGAATACAAAATTTTAAGCTTT
  GGTTAATAGATAATAAGTGTAGTGGAAATTTTTAAATTAACATCTCCCTTATATGTTATA
  TTCCTGAGTTGTTGCTCAAATATCATAAAGAACTTAGTTTATAGCTTTATATAACATTGC
  ATTTTATTTTTGAAAAACAAAAAGTATTGTTATTATTTGAAATGTGAATAATGGTGTTTA
  ATAATATCATCATGGCCAGGCGCGGTAGCTCACACCTGTAATCCCAGCTCTTTGGGATGC
  TGAGGTGGACGGATCACCTGAGGTCAAGAGTTCGAGACAGCCTGACCAACGTGGATAAAC
  CCCATCTCAACTAAAATACAAAATTANCCAGGTGTAGTGGCGCATGCCTGTATTCCCAGC
  TACTCAGGAAGCTGAGGCAGAGAATCGCTTGACACCAGAGGCGAATGTGTGTGAGCGATA
  TCGGCCATTGACTCAGCTGGGCACAGGATGGACTCCCCCAAATATATATATATATATTTA
  TCTACGGATGGGTCCTTATTAGCTACTAGGAAACTATGCGCTGAACTTTGAAGATATTGA
  ATGGATCAAATTTAATGGACACTTATG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006915
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006915.1, NP_008846.1
• RefSeq Size: 3813 bp
• RefSeq ORF: 1053 bp
• Locus ID: 6102
• UniProt ID: O75695
• Cytogenetics: Xp11.3
• Domains: CARP
• Protein Families: Druggable Genome
• Gene Summary: The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly-folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death [provided by RefSeq, Jul 2008]