RP2 (NM_006915) Human Tagged ORF Clone

CAT#: RG208124

RP2 (tGFP-tagged) - Human retinitis pigmentosa 2 (X-linked recessive) (RP2)

Product Images

Circular map for RG208124

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RP2
• Synonyms: DELXp11.3; NM23-H10; NME10; TBCCD2; XRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208124 representing NM_006915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTTCTTCTCCAAGAGACGGAAGGCTGACAAGGAGTCGCGGCCCGAGAACGAGGAGGAGCGGC
  CAAAGCAGTACAGCTGGGATCAGCGCGAGAAGGTTGATCCAAAAGACTACATGTTCAGTGGACTGAAGGA
  TGAAACAGTAGGTCGCTTACCTGGGACGGTAGCAGGACAACAGTTTCTCATTCAAGACTGTGAGAACTGT
  AACATCTATATTTTTGATCACTCTGCTACAGTTACCATTGATGACTGTACTAACTGCATAATTTTTCTGG
  GACCCGTGAAAGGCAGCGTGTTTTTCCGGAATTGCAGAGATTGCAAGTGCACATTAGCCTGCCAACAATT
  TCGTGTGCGAGATTGTAGAAAGCTGGAAGTCTTTTTGTGTTGTGCCACTCAACCCATCATTGAGTCTTCC
  TCAAATATCAAATTTGGATGTTTTCAATGGTACTATCCTGAATTAGCTTTCCAGTTCAAAGATGCAGGGC
  TAAGTATCTTCAACAATACATGGAGTAACATTCATGACTTTACACCTGTGTCAGGAGAACTCAACTGGAG
  CCTTCTTCCAGAAGATGCTGTGGTTCAGGACTATGTTCCTATACCTACTACCGAAGAGCTCAAAGCTGTT
  CGTGTTTCCACAGAAGCCAATAGAAGCATTGTTCCAATATCCCGGGGTCAGAGACAGAAGAGCAGCGATG
  AATCATGCTTAGTGGTATTATTTGCTGGTGATTACACTATTGCAAATGCCAGAAAACTAATTGATGAGAT
  GGTTGGTAAAGGCTTTTTCCTAGTTCAGACAAAGGAAGTGTCCATGAAAGCTGAGGATGCTCAAAGGGTT
  TTTCGGGAAAAAGCACCTGACTTCCTTCCTCTTCTGAACAAAGGTCCTGTTATTGCCTTGGAGTTTAATG
  GGGATGGTGCTGTAGAAGTATGTCAACTTATTGTAAACGAGATATTCAATGGGACCAAGATGTTTGTATC
  TGAAAGCAAGGAGACGGCATCTGGAGATGTAGACAGCTTCTACAACTTTGCTGATATACAGATGGGAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208124 representing NM_006915
  Red=Cloning site Green=Tags(s)
  
  MGCFFSKRRKADKESRPENEEERPKQYSWDQREKVDPKDYMFSGLKDETVGRLPGTVAGQQFLIQDCENC
  NIYIFDHSATVTIDDCTNCIIFLGPVKGSVFFRNCRDCKCTLACQQFRVRDCRKLEVFLCCATQPIIESS
  SNIKFGCFQWYYPELAFQFKDAGLSIFNNTWSNIHDFTPVSGELNWSLLPEDAVVQDYVPIPTTEELKAV
  RVSTEANRSIVPISRGQRQKSSDESCLVVLFAGDYTIANARKLIDEMVGKGFFLVQTKEVSMKAEDAQRV
  FREKAPDFLPLLNKGPVIALEFNGDGAVEVCQLIVNEIFNGTKMFVSESKETASGDVDSFYNFADIQMGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006915
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006915.3
• RefSeq Size: 3813 bp
• RefSeq ORF: 1053 bp
• Locus ID: 6102
• UniProt ID: O75695
• Cytogenetics: Xp11.3
• Domains: CARP
• Protein Families: Druggable Genome
• Gene Summary: The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly-folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death [provided by RefSeq, Jul 2008]