RPP14 (NM_007042) Human Untagged Clone

CAT#: SC115755

RPP14 (untagged)-Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPP14
• Synonyms: P14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115755 sequence for NM_007042 edited (data generated by NextGen Sequencing)
  ATGCCTGCCCCTGCTGCCACATATGAAAGAGTAGTTTACAAAAACCCTTCCGAGTACCAC
  TACATGAAAGTCTGCCTAGAATTTCAAGATTGTGGAGTTGGACTGAATGCTGCACAGTTC
  AAACAGCTGCTTATTTCGGCTGTGAAGGACCTGTTTGGGGAGGTTGATGCCGCCTTACCT
  TTGGACATCCTAACCTATGAAGAGAAGACCTTGTCAGCCATCTTGAGAATATGTAGCAGT
  GGTCTTGTCAAATTGTGGAGCTCTTTGACCCTGTTAGGATCCTATAAAGGCAAAAAATGT
  GCTTTCCGGGTGATTCAGGTTTCTCCATTTCTTCTTGCATTATCTGGTAATAGTAGGGAA
  CTAGTATTGGATTGA
  
  Clone variation with respect to NM_007042.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007042 unedited
  GTATACGACTCACTATAGGCGGCCGCGACATTCGCACGGGGCCAAACGTAAAGACACCAG
  GAGTTTCTCGGGCCCAGCTGTGGCTGCTGCCGGGGAGCCCCAAGCCTTGGCGGGTCCTTG
  CGGCGAATAGGAGTCTGGTCAGGCGTCAGGCTAGTCCGACGAAGAGTGGGTGTGATCAGC
  ACTGGAAAAGATGCCTGCCCCTGCTGCCACATATGAAAGAGTAGTTTACAAAAACCCTTC
  CGAGTACCACTACATGAAAGTCTGCCTAGAATTTCAAGATTGTGGAGTTGGACTGAATGC
  TGCACAGTTCAAACAGCTGCTTATTTCGGCTGTGAAGGACCTGTTTGGGGAGGTTGATGC
  CGCCTTACCTTTGGACATCCTAACCTATGAAGAGAAGACCTTGTCAGCCATCTTGAGAAT
  ATGTAGCAGTGGTCTTGTCAAATTGTGGAGCTCTTTGACCCTGTTAGGATCCTATAAAGG
  CAAAAAAATGTGCTTTCCGGGTGATTCAGGTTTCTCCATTTCTTCTTGCATTATCTGGTA
  ATAGTAGGGAACTAGTATTGGATTGAATGAATAGTCTTCCATTNTGGAAACGTTCATCCA
  CTCTCATATTTATTTTTTTGGTGCCTGCATGTTTGAAGACTGAAGCANGCTAAAAGCTCT
  TGATGAAATTTGAGGGTGCTGAAGATGNTCCCACTAATTTCCAGCCATCACCTTTGTGGG
  GGTTGGGCTTCCGAGGACAGTCTGTCTGACCCTGCCATTGCTGACCCTGCAGCACTTTCA
  GCATATGCCATCAAAGTTGGGAGACCGCGCTGACTTTAAGGAGGCCTTCACACAGACTGG
  AGGGGCCTACCTTCTAGAATACAGGGGGAATGCCATCCTTTGCACTTGAATGAGACTTGC
  AAACACACCAGNTTGGAATACATTGCCCATGGGGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007042 unedited
  CCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTAGAGATGGGGTTTC
  ACCATGTTGGCCAGGCTGGTCTTGGACTCCTGACCTCAAGTGATCCGCCTGCCGCAGCCT
  CCCAAAGTGCCAGGATTACAGGCATGAGCCACCGGGCCCAGCCTACTTCTTGAATTAAAA
  AACAAAAAACAAACAAAAAACCCAGGCAGATAATGAAATCCTGCATACACTCAAGTCTGC
  ATGAAGCATAAGGCCAAACTGGAAAGTGGGCATTTGAACAACCCTCTTCCTGCTCCCTTC
  CCCTAACTAAGATGGGCTTCTGGGCCTATCAACCATTCTTTGGTAAAGAGCAGCAATTCC
  CCATAGGCTCTTTAACAACAGCATTGGTGTTTGAGGCCGCATCTTTAAAACATCTATTTC
  AGGATTTGGAAGCTTCTGGAACCATAACTTTAACCCAGCCTTCCATAACAGCCTTTCTAC
  TTTCTATTACAGAACATGACACTGCAATAATAGCAATGAACCGCTTTAGCTTTTTCACTT
  TTGCAGAACCTAAAACAACTTCTTCAATATATAAAGGGGCTGGAAAGCAAACCTCCCTGG
  AAAGAAAACACAGCCTGGCCCTGCATTTTATTTCTAGGAAAGCTGACAAAGTCCGTTGAT
  CAAAACTCCTGGACAATTCGATTTCCAAAATGGGCGCGTTTGAAAAGCCTTAATTAAACG
  CAAAGGATGACATCCCCTGTAATTTCGAAAAGTACCCCATCACTTTTGGGAAGGCCTCCA
  AGTTAAGGGGCGCCCAAATTTGAGCGCATAGCTGAAGCGCCGATGGGCACCCCTGCGAGT
  TAAAAAACCGCCTTTCAAACCCACCCCCAAAGGGCAGGTCGGAAATAATGGGAACATTTA
  ATACCTCCAATCCATAAGCTTTAACCGGTTTGCTTTAAAATCGCGGCCAAAAATAAATTA
  AGGGGGAAAGGTTCAACGGAACACTTTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007042
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007042.1, NP_008973.1
• RefSeq Size: 1567 bp
• RefSeq ORF: 375 bp
• Locus ID: 11102
• UniProt ID: O95059
• Cytogenetics: 3p14.3
• Domains: MaoC_dehydratas
• Gene Summary: This gene encodes a subunit of ribonuclease P and has 3' to 5' exoribonuclease activity. Transcripts for this gene are bicistronic and include a conserved downstream open reading frame for the hydroxyacyl-thioester dehydratase type 2 (HTD2) gene. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.