DUSP14 (NM_007026) Human Untagged Clone

CAT#: SC115747

DUSP14 (untagged)-Human dual specificity phosphatase 14 (DUSP14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP14
• Synonyms: MKP-L; MKP6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115747 sequence for NM_007026 edited (data generated by NextGen Sequencing)
  ATGAGCTCCAGAGGTCACAGCACGCTACCAAGGACTCTCATGGCCCCTCGGATGATTTCC
  GAGGGAGACATAGGAGGCATTGCTCAAATCACCTCCTCTCTATTCCTGGGCAGAGGCAGT
  GTGGCCTCCAATCGGCACCTCCTCCAGGCTCGTGGCATCACCTGCATTGTTAATGCTACC
  ATTGAGATCCCTAATTTCAACTGGCCCCAATTTGAGTATGTTAAAGTGCCTCTGGCTGAC
  ATGCCGCATGCCCCCATTGGACTGTACTTTGACACCGTGGCTGACAAGATCCACAGTGTG
  AGCAGGAAGCACGGGGCCACCTTGGTGCACTGTGCTGCAGGGGTGAGCCGCTCAGCCACG
  CTGTGTATCGCGTACCTGATGAAATTCCACAACGTGTGCCTGCTGGAGGCGTACAACTGG
  GTGAAAGCCCGGCGACCTGTCATCAGGCCCAACGTAGGCTTCTGGAGGCAACTGATAGAC
  TACGAGCGCCAGCTCTTTGGGAAGTCGACAGTTAAAATGGTACAGACACCTTATGGCATA
  GTTCCCGACGTCTATGAGAAGGAGTCCCGACACCTGATGCCTTACTGGGGGATTTAG
  
  Clone variation with respect to NM_007026.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007026 unedited
  GTCACTAATTTGTAATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGCA
  GGAGGACGGAGCCCTAACCGCAACCCGCGCCGCGCCGCGCCGATTTGATTTGTATCCACT
  GTCACCAGCACTGCTCACTTAGGACTTTCTGGATCCAGACCCAGGCAGCGCACACTGGAC
  TCTTGAGGAAGAAGGAGACTCTAATTTTGGATTCCTTGGTGGAGGAAAATAAAACACTCT
  GGTCTTGCCGCCAACGATGCAAGTGTGACTGCTGGCGTCTTCATGAGCTCCAGAGGTCAC
  AGCACGCTACCAAGGACTCTCATGGCCCCTCGGATGATTTCCGAGGGAGACATAGGAGGC
  ATTGCTCAAATCACCTCCTCTCTATTCCTGGGCAGAGGCAGTGTGGCCTCCAATCGGCAC
  CTCCTCCAGGCTCGTGGCATCACCTGCATTGTTAATGCTACCATTGAGATCCCTAATTTC
  AACTGGCCCCAATTTGAGTATGTTAAAGTGCCTCTGGCTGACATGCCGCATGCCCCCATT
  GGACTGTACTTTGACACCGTGGCTGACAAGATCCACAGTGTGAGCAGGAAGCACGGNGCC
  ACCTTGGTGCACTGTGCTGCAGGGGTGAGCCGCTCAGCCACGCTGTGTATCGCGTACCTG
  ATGAAATTCCACAACGTGTGCCTGCTGGANGCGTACAACTTGGTGAAAGCCCGGCGACCT
  GTCATCANGCCCAACGTANGCTTCTGGAGGCAACTGATAGACTACGAGCGCCACCTCTTT
  GGGAAGTCCACAGTTAAAATGGTACAGACACCCTATGGCATAGTTCCCGACGTCTATGAA
  AAGAGTCCCGACCCCTGATGCCCAACTGGNGGGATTTATGGCCCCTGAACCCTGCGTCAG
  CAGCCCCAAGCGGGGCCGGCATTTTGCTCCCCCCCGTTTGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007026 unedited
  GGCCGCGGCCGCAATCTAGAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTATAAGCATATAAATTTATTAAAAATAATGCTAATATAATCCTGGGCACATGG
  GTTCCAAAAGAAATTTTGCAGCAAATTTCATTATAGTTACTTAACACCTAAATAATAAGG
  GGGTTTTTAACTTACTTACAGACTCCCTAAATAATGGAGGGGAAAGGAAAGAGTAGGGCT
  AATCCACTAAAAACTGAACCTGGTATCAACCTTCCCTAAACATCTGCCTGGCCCGCACCC
  TGGGGCAAAACTTGGTTTTAAAGGGTTAATGAGGCCCCAAAGTGATTTTTGCACTGAGCT
  TCTCCTGCTCCTACCAAGGGGCCCAAGTGCCTGACATTCCCAATAGATCCCAACCAGTTT
  ATTAAACTGTTCAAATTGGTAAAAATGATTAAAAGCCTAAAAATCTTCATCAACCCTCTT
  CCCAAAATGCTAATTCCAAAAACGGACCTACAACGGATGCATCTCCTTTTTTGCCCCCCC
  CCCCTCCCTATCTCTTAAAACAAACTGCAACCCCATGTGCAAAAAACACTTTCAGGGAGA
  ACCAAAACTCAGCCTTTTAAAAAAAAAGCCGATACGGGACCCCACCGCGGGCCACACCAG
  CCCGCGCCACCTCTGGCATTGTTAACTACGTCTTTTCGCCTCCAACTCCCCCCCAAAGGC
  CCAGGCGGTCCGGCACCCCTCCACAAACCGCCGGAACCATGCCCCAGGGGTCTTGAACTT
  TTAAACCGTGCCCTTCCCAAAAACCGTGCCCGTGCTCTAACACTCCACCCCAACCCCTCT
  CTGGGCCCCAACCAAGAGCCCCCACTTTCACCCATCGTACCCTCTCACACGCCCCGCGCG
  GAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007026
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007026.1, NP_008957.1
• RefSeq Size: 1471 bp
• RefSeq ORF: 597 bp
• Locus ID: 11072
• UniProt ID: O95147
• Cytogenetics: 17q12
• Domains: DSPc
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]