RRAS2 (NM_012250) Human Untagged Clone

CAT#: SC115460

RRAS2 (untagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RRAS2
• Synonyms: NS12; TC21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115460 sequence for NM_012250 edited (data generated by NextGen Sequencing)
  ATGGCCGCGGCCGGCTGGCGGGACGGCTCCGGCCAGGAGAAGTACCGGCTCGTGGTGGTC
  GGCGGGGGCGGCGTGGGCAAGTCGGCGCTCACCATCCAGTTCATCCAGTCCTATTTTGTA
  ACGGATTATGATCCAACCATTGAAGATTCTTACACAAAGCAGTGTGTGATAGATGACAGA
  GCAGCCCGGCTAGATATTTTGGATACAGCAGGACAAGAAGAGTTTGGAGCCATGAGAGAA
  CAGTATATGAGGACTGGCGAAGGCTTCCTGTTGGTCTTTTCAGTCACAGATAGAGGCAGT
  TTTGAAGAAATCTATAAGTTTCAAAGACAGATTCTCAGAGTAAAGGATCGTGATGAGTTC
  CCAATGATTTTAATTGGTAATAAAGCAGATCTGGATCATCAAAGACAGGTAACACAGGAA
  GAAGGACAACAGTTAGCACGGCAGCTTAAGGTAACATACATGGAGGCATCAGCAAAGATT
  AGGATGAATGTAGATCAAGCTTTCCATGAACTTGTCCGGGTTATCAGGAAATTTCAAGAG
  CAGGAATGTCCTCCTTCACCAGAACCAACACGGAAAGAAAAAGACAAGAAAGGCTGCCAT
  TGTGTCATTTTCTAG
  
  Clone variation with respect to NM_012250.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012250 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGCGCTACCG
  CGCTGACCGGCCCTCCAGACGCCTCCCGGTACCCGGGACCCCAGCCCGGCCGCTCGCCCG
  CAGCCCGCCGGCCGCACACGTCCCCGGAGCCGGGCCTAGGGCGGGCGGCAGCGGCGGCTC
  GGCGCAGTCAGGCTGGGCTCTGTAGCGTCCCCATGGCCGCGGCCGGCTGGCGGGACGGCT
  CCGGCCAGGAGAAGTACCGGCTCGTGGTGGTCGGCGGGGGCGGCGTGGGCAAGTCGGCGC
  TCACCATCCAGTTCATCCAGTCCTATTTTGTAACGGATTATGATCCAACCATTGAAGATT
  CTTACACAAAGCAGTGTGTGATAGATGACAGAGCAGCCCGGCTAGATATTTTGGATACAG
  CAGGACAAGAAGAGTTTGGAGCCATGAGAGAACAGTATATGAGGACTGGCGAAGGCTTCC
  TGTTGGTCTTTTCAGTCACAGATAGAGGCAGTTTTGAAGAAATCTATAAGTTTCAAAGAC
  AGATTCTCAGAGTAAAGGATCGTGATGAGTTCCCAATGATTTTAATTGGTAATAAAGCAG
  ATCTGGATCATCAAAGACAGGTAACACAGGAAGAAGGACAACAGTTAGCACGGCAGCTTA
  AGGTAACATACATGGAGGCATCAGCAAAGATTAGGATGAATGTAGATCAAGCTTTCCATG
  AACCTGTCCGGGTTATCANGAAATTTCAAGAGCANGAATGTCCTCCTTCACCAGAACCAA
  CACGGAAAGAANAAGACAGAANAGGCTGCCCATTGTGTCATTNTCTAGAATCCCTTCAGT
  TTTAGCTACCAACGGCAGNAAAAGCCCTCATCTCTCTNNTCTCTCCTCAGTTAATNCNNT
  GTTGGTACTTNTCTAGCCTAGACAAATGATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012250
• Insert Size: 2330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012250.3, NP_036382.2
• RefSeq Size: 1510 bp
• RefSeq ORF: 615 bp
• Locus ID: 22800
• UniProt ID: P62070
• Cytogenetics: 11p15.2
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).