GCP4 (TUBGCP4) (NM_014444) Human Untagged Clone

CAT#: SC115032

TUBGCP4 (untagged)-Human tubulin, gamma complex associated protein 4 (TUBGCP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCP4
• Synonyms: 76P; GCP-4; GCP4; Grip76; MCCRP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014444 edited
  GAATTCGGCACGAGGGGAAGGGGAAGCACTCCCCTCGTGGTCGCCTGGAGGTGCGCTGGA
  GGAGGGGGTGACATAACCAGGGACTCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTC
  TTGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTGGCCTGCAG
  GTATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGC
  CGGCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTG
  CAACAGCAGGATCACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTG
  CGGGCCTTCTGCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGAT
  TTGGAACAAGAGTTCCTGGGTGATCCCCATCTCTCCATATCACATGTCAACTACTTCCTA
  GACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAA
  AAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCT
  CCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAG
  CTCTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAA
  CAGGGGCCATCTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGC
  ATTGGGGGACTGACAGGAAAACAACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAA
  GAGAACATGCTGGCACCATCTCTGAAGCAGTTTTCCCTACGAGTGGAGATTTTGCCATCC
  TACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGAGAATCTGTCCAGATGTTT
  GAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAAACCAGGAAGACACT
  TTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAA
  CAGGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTAGAA
  GAATCCGATTTACTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGA
  GAACTGTTTCAGGCCTTCATTGACACAGCTCAACACATGTTGAAAACACCACCCACTGCA
  GTAACTGAGCATGATGTGAATGTGGCCTTTCAACAGTCAGCACACAAGGTATTGCTAGAT
  GATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTATCACGGAAAGGAGCACAAA
  GCAGATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTCCCCGGGAAGCCCCT
  GCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGCCACTACATATTCTC
  TTCACCCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTGAGTGTGCGC
  CGGGTGCAAGCTGAGCTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAG
  TCGAACCAGACTGATGCAATCAAGTGGCGCCTAAGAAATCACATGGCATTTTTGGTGGAT
  AATCTTCAGTACTATCTCCAGGTAGATGTGTTGGAGTCTCAGTTCTCCCAGCTGCTTCAT
  CAGATCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTCATGACCACTTCCTGAGC
  AATTTGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTCACTGCCTGAATGAAATC
  CTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACCTAGGCCCACTGGATGAG
  CGTGGAGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGCCAGTCTTCACTCCTG
  TTCAAGATTCTCTCCAGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAACTACTG
  TTACGACTAGATTATAACAAATACTATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGG
  ATGTGAAAATTTCTGGCTCATAAATTGAAATAACAGCCACGTTCCCAAGGTTGTAACAGA
  AGATTCAAAACATCCCATTCTAGCCACACACAAATAAATATCTGCGGCTTAGTGATAGGA
  CTCTACCTTTTCTCCTAGAAGCAGTTACTGAACATCCAGGAGTACAACTCCTTCCCATCA
  TTCCCATGTGGAAGGGTCTCTCCCATCAAGGAGAACATGTGGCATCTCTGATCCTTTACA
  TTGAGAACATTTGTTGGATATGTTCATTTATTCAATAGTCATTTATTGAGCACCTACTAC
  GTACCTTGGTACTGTTCAAGCTGTGGGAGATACAGCGGTAAACAAACAATATAGAGCAGA
  AAGTTAAATATTTTATGGTTCATATGTGAAAAAGTAATTATGTTTATAAATAGACTAACT
  GCTGGATGTTACCACCAAGTAAGAAAGCAACAGGTAAGATAGGCTTTCTCTCTCCCTATA
  CCAAGTAATTTATACCTACACAGATTGGGCAATTCTAGCTAATGAAAATATACTTAAAAG
  TATTTCTTGGGCCGGGCATGGTGGCTCACGCCTGTGGTCCCGGCACTTTGGGAGGCCGGG
  GCGGGCGGATCGCCTGAGGTCGGGAGTTTGGGACCAGCCTGACCAACATGATGAAACCTC
  GATTCTGCTGGGAATGCAAGGATTGGCCGGGTGTGGTGGCATGTGCCTGTGGTCCCAGCT
  GCTCAGGGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014444 unedited
  TATATTATGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGGGGA
  AGCACTCCCCTCGTGGTCGGCTGGAGGTGCGCTGGAGGAGGGGGTGACATAACCAGGGAC
  TCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTCTTGGCTCTGAGCGGGTACCCTGGG
  TCCATTTTCACCTGGAACAAGCGGAGTGGCCTTTTTGTATCGCAGGACTTCCCTTTCCTG
  CACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCCGGCTCGGCACAGACTATATTCGC
  TTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACAGCAGGATCACCATCCATCT
  CAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGCCTTCTGCACAGGGCTGGAT
  TCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGAT
  CCCCATCTCTCCATATCACATGTCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCC
  TCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTG
  GAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCTCCTGTTCGAAGTGCACTGGANAAA
  AATCCTGGCCGTTTGTCATGGGTCATGTATAAACAGCTCTCAGCCTGGATGCTCCATGGA
  CTCCTCTTGGACCAGCTTGAAGAATTCTTATCAAACAGGGGCCATCTTCTGGTAATGTCA
  GTGCCCAGCCACGAGAAGACGANGAGGATCTGGGCATTGNNGGACTGACAGGAAAACAAC
  TGAGAGAACTGCANGACTTGCGCCTGATTGAGGAAGAGACATGCNTGCACCATCTTCTGA
  GCAGTTTCCCTACAGTGGAGATTTTGCATCCTACATTCAGTGAGGTGCTGAAAAATCCTA
  TTGTTGGAGATCTGCCACAGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014444 unedited
  TGGTTTGATGAGCGGCGATANCGATTTTCGAGTTTTTTTTTTTTTTTTTTCCCTGAGCAG
  CTGGGACCACAGGCACATGCCACCACACCCGGCCAATCCTTGCATTCCCAGCAGAATCGA
  GGTTTCATCATGTTGGTCAGGCTGGTCCCAAACTCCCGACCTCAGGCGATCCGCCCGCCC
  CGGCCTCCCAAAGTGCCGGGACCACAGGCTTTAGCCACCATGCCCGGCCCAAGAAATACT
  TTTAAGTATATTTTCATTAGCTAGAATTGCCCAATCTGTGTAGGTATAAATTACTTGGTA
  TAGGGAGAGAGAAAGCCTATCTTACCTGTTGCTTTCTTACTTGGTGGTAACATCCAGCAG
  TTAGTCTATTTATAAACATAATTACTTTTTCACATATGAACCATAAAATATTTAACTTTC
  TGCTCTATATTGTTTGTTTACCGCTGTATCTCCCACAGCTTGAACAGTACCAAGGTACGT
  AGTAGGTGCTCAATAAATGACTATTGAATAAATGAACATATCCCACAAATGTTCTCAATG
  TAAAGGATCAGAGATGCCACATGTTCTCCTTGATGGGAGAGACCCTTCCCCTGGGAATGA
  TGGGAAGGATTGTACTCTGGATGTTCAATAACTGCGTTTAGAAAAAAGGTTAAGTCCATC
  ACTAACCCCCAAATTTTTATTTGTGTGCGGCTTCAATGGGTCGTTTTTAATTTTTTGTTA
  CAACCTGGGAAACCGGGTTTTTTTTCATTTTTTAACCCAAATATTTCCCATTCGGAAACT
  CCCCAAATTTCCCCCCCCGGGATAAGCTCTTCGTTTACTTAATCTACACCGATTCGGCCC
  CAATGGAGTGTGCGCGGGGTTCCCAACACCGGAAAATTTTCAACCGGCGAAAACTGGGGG
  TAACACCTCATCAAATTGCTCCCTGCGCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014444
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014444.2, NP_055259.2
• RefSeq Size: 3087 bp
• RefSeq ORF: 2001 bp
• Locus ID: 27229
• UniProt ID: Q9UGJ1
• Cytogenetics: 15q15.3
• Domains: Spc97_Spc98
• Gene Summary: This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (b) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.