GCP4 (TUBGCP4) (NM_014444) Human Tagged ORF Clone

CAT#: RC200872

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TUBGCP4 (Myc-DDK-tagged)-Human tubulin, gamma complex associated protein 4 (TUBGCP4)



  "NM_014444" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TUBGCP4
Synonyms 76P; GCP-4; GCP4; Grip76; MCCRP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200872 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCACGAACTGCTCTTGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTG
GCCTGCAGGTATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCCG
GCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACAGCAGGAT
CACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGCCTTCTGCACAGGGCTGG
ATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGATCCCCATCT
CTCCATATCACATGTCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTA
GAACAAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGG
GGTTGCCTCCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAGCT
CTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAACAGGGGCCATCT
TCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGCATTGGGGGACTGACAGGAAAAC
AACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAAGAGAACATGCTGGCACCATCTCTGAAGCAGTT
TTCCCTACGAGTGGAGATTTTGCCATCCTACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGA
GAATCTGTCCAGATGTTTGAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAAACCAGG
AAGACACTTTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAACA
GGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTAGAAGAATCCGATTTA
CTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGAGAACTGTTTCAGGCCTTCATTG
ACACAGCTCAACACATGTTGAAAACACCACCCACTGCAGTAACTGAGCATGATGTGAATGTGGCCTTTCA
ACAGTCAGCACACAAGGTATTGCTAGATGATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTAT
CACGGAAAGGAGCACAAAGATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTCCCCGGGAAG
CCCCTGCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGCCACTACATATTCTCTTCAC
CCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTGAGTGTGCGCCGGGTGCAAGCTGAG
CTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAGTCGAACCAGACTGATGCAATCAAGT
GGCGCCTAAGAAATCACATGGCATTTTTGGTGGATAATCTTCAGTACTATCTCCAGGTAGATGTGTTGGA
GTCTCAGTTCTCCCAGCTGCTTCATCAGATCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTCAT
GACCACTTCCTGAGCAATTTGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTCACTGCCTGAATG
AAATCCTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACCTAGGCCCACTGGATGAGCGTGG
AGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGCCAGTCTTCACTCCTGTTCAAGATTCTCTCC
AGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAACTACTGTTACGACTAGATTATAACAAATACT
ATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGGATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200872 protein sequence
Red=Cloning site Green=Tags(s)

MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNRLCRLGTDYIRFTEFIEQYTGHVQQQD
HHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVV
EQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPS
SGNVSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKILFVG
ESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDL
LGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEY
HGKEHKDATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAE
LQHCWALQMQRKHLKSNQTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAH
DHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILS
SVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014444
ORF Size 1998 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014444.5
RefSeq Size 4178 bp
RefSeq ORF 2001 bp
Locus ID 27229
UniProt ID Q9UGJ1
Cytogenetics 15q15.3
Domains Spc97_Spc98
MW 76 kDa
Gene Summary This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

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