GCP4 (TUBGCP4) (NM_014444) Human Tagged ORF Clone

CAT#: RG200872

TUBGCP4 (tGFP-tagged) - Human tubulin, gamma complex associated protein 4 (TUBGCP4)

Product Images

Circular map for RG200872

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GCP4
• Synonyms: 76P; GCP-4; GCP4; Grip76; MCCRP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200872 representing NM_014444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCACGAACTGCTCTTGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTG
  GCCTGCAGGTATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCCG
  GCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACAGCAGGAT
  CACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGCCTTCTGCACAGGGCTGG
  ATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGATCCCCATCT
  CTCCATATCACATGTCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTA
  GAACAAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGG
  GGTTGCCTCCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAGCT
  CTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAACAGGGGCCATCT
  TCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGCATTGGGGGACTGACAGGAAAAC
  AACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAAGAGAACATGCTGGCACCATCTCTGAAGCAGTT
  TTCCCTACGAGTGGAGATTTTGCCATCCTACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGA
  GAATCTGTCCAGATGTTTGAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAAACCAGG
  AAGACACTTTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAACA
  GGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTAGAAGAATCCGATTTA
  CTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGAGAACTGTTTCAGGCCTTCATTG
  ACACAGCTCAACACATGTTGAAAACACCACCCACTGCAGTAACTGAGCATGATGTGAATGTGGCCTTTCA
  ACAGTCAGCACACAAGGTATTGCTAGATGATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTAT
  CACGGAAAGGAGCACAAAGATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTCCCCGGGAAG
  CCCCTGCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGCCACTACATATTCTCTTCAC
  CCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTGAGTGTGCGCCGGGTGCAAGCTGAG
  CTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAGTCGAACCAGACTGATGCAATCAAGT
  GGCGCCTAAGAAATCACATGGCATTTTTGGTGGATAATCTTCAGTACTATCTCCAGGTAGATGTGTTGGA
  GTCTCAGTTCTCCCAGCTGCTTCATCAGATCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTCAT
  GACCACTTCCTGAGCAATTTGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTCACTGCCTGAATG
  AAATCCTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACCTAGGCCCACTGGATGAGCGTGG
  AGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGCCAGTCTTCACTCCTGTTCAAGATTCTCTCC
  AGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAACTACTGTTACGACTAGATTATAACAAATACT
  ATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200872 representing NM_014444
  Red=Cloning site Green=Tags(s)
  
  MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNRLCRLGTDYIRFTEFIEQYTGHVQQQD
  HHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVV
  EQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPS
  SGNVSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKILFVG
  ESVQMFENQNVNLTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDL
  LGQLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEY
  HGKEHKDATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAVLEKYNVVFKYLLSVRRVQAE
  LQHCWALQMQRKHLKSNQTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAH
  DHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFKILS
  SVRNHQINSDLAQLLLRLDYNKYYTQAGGTLGSFGM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014444
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014444.5
• RefSeq Size: 3087 bp
• RefSeq ORF: 2001 bp
• Locus ID: 27229
• UniProt ID: Q9UGJ1
• Cytogenetics: 15q15.3
• Domains: Spc97_Spc98
• Gene Summary: This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]