CEP104 (NM_014704) Human Untagged Clone

CAT#: SC114917

CEP104 (untagged)-Human centrosomal protein 104kDa (CEP104)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CEP104
• Synonyms: CFAP256; GlyBP; JBTS25; KIAA0562; ROC22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114917 sequence for NM_014704 edited (data generated by NextGen Sequencing)
  ATGCCCCACAAGATTGGATTTGTAGTCGTCAGCTCATCTGGACACGAAGACGGCTTCAGT
  GCCCGGGAGCTCATGATCCACGCGCCAACTGTCAGTGGGTGGCGGTCACCTAGATTTTGC
  CAGTTTCCACAAGAAATTGTCCTTCAAATGGTGGAGAGATGTCGAATAAGGAAACTGCAG
  TTACTTGCTCACCAGTATATGATTTCAAGTAAAATTGAGTTCTACATTAGTGAAAGCTTG
  CCTGAATATTTTGCACCCTATCAAGCAGAGCGGTTTCGAAGACTTGGCTACGTGTCTCTC
  TGTGATAATGAAAAGACAGGTTGCAAAGCCCGGGAACTAAAATCAGTTTATGTGGATGCA
  GTAGGACAATTTCTTAAACTGATTTTTCACCAAAACCATGTCAACAAATACAACATATAT
  AATCAGGTTGCTTTGGTTGCCATAAATATCATTGGAGACCCTGCAGATTTCAGTGATGAA
  AGCAATACTGCCTCTCGAGAGAAGTTGATTGACCACTACCTTGGGCACAACAGCGAGGAC
  CCTGCTCTAGAAGGAACGTACGCCAGGAAATCTGACTATATCTCTCCGCTAGATGACTTA
  GCTTTTGATATGTACCAAGATCCAGAAGTTGCACAGATCATACGAAAATTAGATGAAAGA
  AAACGGGAAGCTGTCCAAAAGGAACGCTATGATTATGCCAAGAAACTAAAACAAGCCATT
  GCTGATTTGCAAAAGGTTGGTGAACGCCTTGGGAGGTATGAGGTAGAGAAACGCTGTGCC
  GTGGAGAAGGAAGACTACGATCTCGCCAAGGAGAAGAAGCAGCAGATGGAGCAGTATCGT
  GCCGAGGTGTACGAGCAGCTGGAGCTGCACAGCCTCCTGGATGCCGAGCTGATGCGAAGA
  CCTTTTGATTTGCCCCTCCAGCCCCTCGCTCGTTCTGGCAGTCCTTGCCACCAAAAGCCA
  ATGCCCTCACTACCACAACTGGAAGAAAGAGGAACAGAAAACCAGTTTGCAGAACCTTTC
  CTTCAGGAAAAGCCTTCATCATATTCTCTAACTATTTCTCCTCAGCATTCTGCAGTAGAC
  CCGTTACTCCCTGCCACAGATCCTCATCCAAAGATCAATGCAGAGTCCCTGCCCTACGAT
  GAGCGGCCTCTTCCAGCTATTCGTAAGCATTATGGGGAGGCAGTGGTGGAGCCGGAAATG
  AGTAATGCAGACATCAGCGATGCTCGGAGGGGAGGCATGTTAGGGGAGCCAGAGCCCTTA
  ACCGAGAAGGCCTTGAGAGAAGCCAGCTCTGCCATCGATGTGTTGGGAGAAACCTTGGTT
  GCTGAGGCCTATTGTAAGACGTGGTCCTACCGAGAGGATGCACTGCTTGCCTTGTCTAAG
  AAGTTAATGGAAATGCCTGTTGGAACCCCAAAAGAAGATTTAAAGAACACACTGAGAGCA
  TCCGTCTTTCTCGTTAGAAGAGCCATAAAGGACATTGTGACCTCCGTTTTTCAGGCTTCT
  TTGAAATTGTTGAAAATGATCATTACACAATATATTCCTAAACATAAACTGAGTAAACTT
  GAAACAGCTCACTGTGTGGAGAGGACCATTCCCGTTTTGCTCACCAGAACTGGAGATTCT
  TCTGCCCGCCTCCGCGTCACAGCTGCAAATTTTATTCAGGAAATGGCCTTGTTTAAAGAA
  GTTAAGTCTCTCCAAATTATTCCATCCTACCTGGTGCAGCCATTGAAAGCAAACTCTTCA
  GTTCACCTGGCAATGAGTCAGATGGGCCTCCTGGCCCGGCTGCTGAAAGACCTGGGCACT
  GGCAGCTCGGGCTTCACCATTGACAACGTGATGAAGTTTTCAGTGAGTGCCCTGGAGCAT
  AGAGTGTATGAGGTCCGCGAGACGGCGGTTCGAATTATTTTGGACATGTACAGACAGCAC
  CAGGCTTCCATCCTGGAGTACCTTCCTCCAGACGACAGCAACACACGCAGGAACATTCTC
  TACAAAACAATTTTTGAGGGATTTGCTAAAATAGATGGCAGAGCTACAGATGCTGAGATG
  AGGGCACGGAGAAAAGTGGCTACAGAAGAAGCAGAAAAACAAAAGAAAGAAGAAATAAAA
  GCTTTACAAGGGCAGCTGGCAGCACTGAAAGAAATTCAGGCTGAAGTTCAGGAAAAAGAA
  AGTGATGCTGTGAAGCCAAAGAATCAGGACATTCAAGGAGGGAAAGCAGCCCCTGCTGAA
  GCTCTGGGAATCCCGGATGAGCACTATCTAGATAATTTGTGTATTTTTTGTGGGGAAAGG
  AGTGAATCCTTCACAGAGGAAGGTCTGGATCTCCACTACTGGAAGCACTGTCTCATGCTG
  ACAAGATGTGACCACTGCAAACAGGTGGTCGAGATATCCAGTCTGACGGAGCACTTGCTG
  ACGGAATGTGACAAAAAAGACGGGTTTGGAAAGTGTTACCGTTGCAGTGAGGCTGTTTTC
  AAGGAAGAGCTGCCCAGACACATAAAACACAAGGATTGCAACCCTGCCAAACCGGAGAAG
  CTGGCAAACCGGTGTCCCCTGTGTCATGAGAACTTCAGCCCTGGAGAAGAGGCATGGAAA
  GCTCACCTGATGGGCCCAGCCGGCTGCACGATGAACCTGCGCAAGACACACATTCTGCAG
  AAGGCCCCGGCACTGCAGCCAGGGAAAAGCTCAGCTGTGGCCGCATCAGGCCCCTTGGGG
  TCAAAGGCCGGAAGCAAGATCCCCACCCCGAAGGGCGGACTGAGCAAGAGCTCCAGCAGG
  ACGTACGCCAAGCGCTGA
  
  Clone variation with respect to NM_014704.3
  2057 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014704 unedited
  ATTCGGCACGAGGTGGAGGCTGCGGCCGGGGCTATGGGCAGAGGTGGCCGGGTCTGCTTC
  TTCCCTGTCACTGCCGCAGCAGCTTGGGGTGCAAGGCGGCCTGGGGCGGCGGAAGGGGCG
  CCCCCCGAGTTCCGTCTGAGGAAGCGGCGGCTGCCGAGGAAGTCCACGAGAGCCGGGTCC
  CGCACGCCCCTGGGGTCCGGGTGCTGAGGCGCCGCCTGGCCGCCTCCCCGGGCCGCGGCA
  CCGCTCCGACCCAGCCGCCCTCTCCACCGGGGCCGCGGCAGCGCCAGTCGGGGCCCTTCC
  CTCTAAGCAGCCCAAACGTGCAGAATGCCCCACAAGATTGGATTTGTAGTCGTCAGCTCA
  TCTGGACACGAAGACGGCTTCAGTGCCCGGGAGCTCATGATCCACGCGCCAACTGTCAGT
  GGGTGGCGGGCACCTAGATTTTGCCAGTTTCCACAAGAAATTGTCCTTCAAATGGTGGAG
  AGATGTCCAATAACGAAACTGCAGTTACTTGCTCACCAGTATATGATTTCAAGTAAAATT
  GAGGTCTACCTTACTGAAAGCTTGCCTGAATATTTTGCACCCTATCAGCAGAGCGGTTTC
  AAGACTTGGCTACGTGGCTCTCTGTGATATGGAAAGCAAGTTGCAAGCCCCGGAACTAAA
  ATAGTTTATGTGATGCAGAGGACAATTTTTAACCGATTTTACCCAACCATGGTACAAAAC
  ACCATTATATCCGGTGGTTTGGTGCATAATATATTGGGGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014704 unedited
  CTTGGACCCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAGGNCTCT
  TTTGCCTTTATTTCTTGCTATAGTGGGAACGTGAGTTCTGCTACCTGGGGCTGAGTGCAC
  TGGGCAGCAGGCCCAGCAGACGGGGCCTGCAGGGGGAAGGCACGGCTGGAGGCCCGTGCG
  CACCGCCACTCTTCTGGAACCGCTGCTGGCCGAGCCACAGCCCTGCGGAAATTCTACTGC
  GTGTGGCAGAGACCGATGTGCAAAAGGTTTCTGGGGAATGTTTTATGGAAATTGCTCCAA
  GCACTAAGGAAAGCGGGACCGTGCCTGTGCTGACCGTGGCCTTGCGCGACCGCCTCCCTG
  GCGGGGCTCTTTGGCTCTCTTCAAACAGGACGCTTCCTTCTCTGATTCTAAGGAAGGCCT
  GACACACCCCTGAAGGCTTAATGTACAGCGCGGCCCGGTCCTGGCCCTGGGCCCATGACC
  CTTGGCTCGCTGCATCCATAACGGTTGCACCAGCATCTCACGGCTCAGACCGCCATACAC
  GCCTCCCCGCGGCCCGTCTTTTGCCTCCGAGTCCCGCCGTGTCGGGCCCGCGCGTCGGCG
  TTCCGTCGCCCTNTCCGTTCAACGTGGTCCCCGCGCGGCTCCGTCTCGTGCCCCCCCCCC
  CGACTTCCCCCCGCCCTCTTTTTCGTATTTCCCCCCATCCCTTCCCCCGCTCCTTCTTTC
  CTCCCCCTTTTCCTCTCACTTCCGCGTCCTTCCTATCTCGCCTAGCCATTCCGTCCTCCC
  GTCTTTTTCTCTCCCCGCCCCCTCCTTTTTTCCGTTTTCCCCCCCCCCGCGTTCTCCATC
  CCCCATTCCCCCGCCCCCCCTGCCGCCCTCCTTCCCCCCGCCCCCCGCGTCCCCCCCTTG
  GTAATCCTGTCCGGCCCCCTGACCGTCCTCCCCCTTTTCTCTCCCCATTTTCATTTTTTT
  TCTCCTACCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014704
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014704.1, NP_055519.1
• RefSeq Size: 5848 bp
• RefSeq ORF: 2778 bp
• Locus ID: 9731
• UniProt ID: O60308
• Cytogenetics: 1p36.32
• Gene Summary: This gene encodes a centrosomal protein required for ciliogenesis and for ciliary tip structural integrity. The mammalian protein contains three amino-terminal hydrophobic domains, two glycosylation sites, four cysteine-rich motifs, and two regions with homology to the glutamate receptor ionotropic, NMDA 1 protein. During ciliogenesis, the encoded protein translocates from the distal tips of the centrioles to the tip of the elongating cilium. Knockdown of the protein in human retinal pigment cells results in severe defects in ciliogenesis with structural deformities at the ciliary tips. Allelic variants of this gene are associated with the autosomal-recessive disorder Joubert syndrome, which is characterized by a distinctive mid-hindbrain and cerebellar malformation, oculomotor apraxia, irregular breathing, developmental delay, and ataxia. [provided by RefSeq, Feb 2016]