MELK (NM_014791) Human Untagged Clone

CAT#: SC114824

MELK (untagged)-Human maternal embryonic leucine zipper kinase (MELK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MELK
• Synonyms: HPK38
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014791 edited
  ATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTATTGGGACAGGT
  GGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGTAGCTATAAAA
  ATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGAGATTGAGGCC
  TTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGAGACAGCCAAC
  AAAATATTCATGGTTCTTGAGTACTGCCCTGGAGGAGAGCTGTTTGACTATATAATTTCC
  CAGGATCGCCTGTCAGAAGAGGAGACCCGGGTTGTCTTCCGTCAGATAGTATCTGCTGTT
  GCTTATGTGCACAGCCAGGGCTATGCTCACAGGGACCTCAAGCCAGAAAATTTGCTGTTT
  GATGAATATCATAAATTAAAGCTGATTGACTTTGGTCTCTGTGCAAAACCCAAGGGTAAC
  AAGGATTACCATCTACAGACATGCTGTGGGAGTCTGGCTTATGCAGCACCTGAGTTAATA
  CAAGGCAAATCATATCTTGGATCAGAGGCAGATGTTTGGAGCATGGGCATACTGTTATAT
  GTTCTTATGTGTGGATTTCTACCATTTGATGATGATAATGTAATGGCTTTATACAAGAAG
  ATTATGAGAGGAAAATATGATGTTCCCAAGTGGCTCTCTCCCAGTAGCATTCTGCTTCTT
  CAACAAATGCTGCAGGTGGACCCAAAGAAACGGATTTCTATGAAAAATCTATTGAACCAT
  CCCTGGATCATGCAAGATTACAACTATCCTGTTGAGTGGCAAAGCAAGAATCCTTTTATT
  CACCTCGATGATGATTGCGTAACAGAACTTTCTGTACATCACAGAAACAACAGGCAAACA
  ATGGAGGATTTAATTTCACTGTGGCAGTATGATCACCTCACGGCTACCTATCTTCTGCTT
  CTAGCCAAGAAGGCTCGGGGAAAACCAGTTCGTTTAAGGCTTTCTTCTTTCTCCTGTGGA
  CAAGCCAGTGCTACCCCATTCACAGACATCAAGTCAAATAATTGGAGTCTGGAAGATGTG
  ACCGCAAGTGATAAAAATTATGTGGCGGGATTAATAGACTATGATTGGTGTGAAGATGAT
  TTATCAACAGGTGCTGCTACTCCCCGAACATCACAGTTTACCAAGTACTGGACAGAATCA
  AATGGGGTGGAATCTAAATCATTAACTCCAGCCTTATGCAGAACACCTGCAAATAAATTA
  AAGAACAAAGAAAATGTATATACTCCTAAGTCTGCTGTAAAGAATGAAGAGTACTTTATG
  TTTCCTGAGCCAAAGACTCCAGTTAATAAGAACCAGCATAAGAGAGAAATACTCACTACG
  CCAAATCGTTACACTACACCCTCAAAAGCTAGAAACCAGTGCCTGAAAGAAACTCCAATT
  AAAATACCAGTAAATTCAACAGGAACAGACAAGTTAATGACAGGTGTCATTAGCCCTGAG
  AGGCGGTGCCGCTCAGTGGAATTGGATCTCAACCAAGCACATATGGAGGAGACTCCAAAA
  AGAAAGGGAGCCAAAGTGTTTGGGAGCCTTGAAAGGGGGTTGGATAAGGTTATCACTGTG
  CTCACCAGGAGCAAAAGGAAGGGTTCTGCCAGAGACGGGCCCAGAAGACTAAAGCTTCAC
  TATAATGTGACTACAACTAGATTAGTGAATCCAGATCAACTGTTGAATGAAATAATGTCT
  ATTCTTCCAAAGAAGCATGTTGACTTTGTACAAAAGGGTTATACACTGAAGTGTCAAACA
  CAGTCAGATTTTGGGAAAGTGACAATGCAATTTGAATTAGAAGTGTGCCAGCTTCAAAAA
  CCCGATGTGGTGGGTATCAGGAGGCAGCGGCTTAAGGGCGATGCCTGGGTTTACAAAAGA
  TTAGTGGAAGACATCCTATCTAGCTGCAAGGTATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014791 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGTCTCTCAG
  GACAGCAGGCCCCTGTCCTTCTGTCGGGCGCCGCTCAGCCGTGCCCTCCGCCCCTCAGGT
  CAGTTCTCCCGCCTGCCCGCCGCAGTCGCAGTTGATGGCTGGGGTCTGGGCTGTGCGGGG
  CGCAGCGGCCCAAACCCAGTTTGCTCCTGGCTCTCGGGAGACTGGAGGATTTCATCGGAG
  CCCCGCGCTTTACCAGCCCTGTTCCCTGGATAAGATATTTGACCTTTCCGACCCGCGGTT
  TTCTTCTCCAATCTCGCTCCGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCAC
  TGCAACCTCTGCCTCCTGCCTCAGCCTCCCAAGTTCTTTTTCTAATTCCAAATAAACTTG
  CAAGAGGACTATGAAAGATTATGATGAACTTCTCAAATATTATGAATTACATGAAACTAT
  TGGGACAGGTGGCTTTGCAAAGGTCAAACTTGCCTGCCATATCCTTACTGGAGAGATGGT
  AGCTATAAAAATCATGGATAAAAACACACTAGGGAGTGATTTGCCCCGGATCAAAACGGA
  GATTGAGGCCTTGAAGAACCTGAGACATCAGCATATATGTCAACTCTACCATGTGCTAGA
  GACAGCCAACAAAATATTCATGGTTCTTGAGTACTGCCCTGGNAGAGAGCTGTTTGACTA
  TATAAATTTCCAGGATCGCCTGTCAGAAGAGAGACCCGGGTGTCTTCCGTCAGATATATC
  TGCTGTTGCTTATGTGCACAGCCAAGGCTTTGCTCACAGGGACCTCAAGCAGAAAATTGC
  TGTTTGATGATATCATAAATAAAGCTGATGACTTTGGTCTTGTGCAAACCCAAGGTACAA
  C
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014791 unedited
  GTTTTATTTCAGTTAGNAATTACACATTACAAANAGACATAGTTAAGAGCTTACATTCAC
  ATATGGAAGTGAATCTAAGAAAGTCAACAATTATTAATATACAAAACCACATGATTAAAA
  AAGACAGTAACATAATGACAGATGGGCTTGATTTAGATTCATACACAAAATAATATCTTT
  TGTTTAAAAACAAAGAGATATTGGTCTTAAGATAGCTCTTTAGAAACAAGTTGGTAGTTC
  CAATGAACTTTAAAATCAAAGCGATCATAACAGTCTTTATGTAGGCTGTATCACACCCAC
  ACTCATCCGGCAGGATGGAAGAATCCATCAATTATACCTTGCAGCTAGATAGGATGTCTT
  CCACTAATCTTTTGTAAACCCAGGCATCGCCCTTAAGCCGCTGCCTCCTGATACCCACCA
  CATCGGGTTTTTGAAGCTGGCACACTTCTAATTCAAATTGCATTGTCACTTTCCCAAAAT
  CTGACTGTGTTTGACACTTCAGTGTATAACCCTTTTGTACAAAGTCAACATGCTTCTTTG
  GAAGAATAGACATTATTTCATTCAACAGTTGATCTGGATTCACTAATCTAGTTGTAGTCA
  CATTATAGTGAAACTTTAGTCTTCTGGGCCCGTCTCTGGCAGAACCCTTCCTTTTGTTCC
  TGGTGAGCACAGTGATTACCTTATCCAACCCCCTTTAATGCTCCCAACCACTTTTGGTCC
  CTTTCTTTTTGAAGCCTTCTCATATGGGCCTTGGTTAAATCCAATTCCCTGACCGGCCCC
  TCTTTTAGGGGTAACGACCCCGTCATTAACTCGCTTTCCTCGCCGATTTATCGGGTTTCA
  ATCGGGCGTTGTTTAAGCAACGTCTTCTTTCCTCGAAGTGGTTCGCTCACATTTTCGTCG
  CTCGTCTTCGTCTTTCTCTGGCCGTTATACACCGAGCTTGGCTCAGTATACACACTATTT
  TTTTCTACGTTGTTCCCCGTTTACCTTTTCCTTCTTTTCTCTCCCTCTTTCCCCTTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014791
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014791.2, NP_055606.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1956 bp
• Locus ID: 9833
• UniProt ID: Q14680
• Cytogenetics: 9p13.2
• Domains: pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).