DDX46 (NM_014829) Human Untagged Clone

CAT#: SC114794

DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX46
• Synonyms: Prp5; PRPF5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014829, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGAA
  GTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGATAG
  GAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGT
  TCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAA
  GGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAG
  ATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGATGACAAG
  GAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGAAAGCGAA
  AAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAACTGAAAAA
  GGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCT
  GCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAG
  AAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAGAAGTC
  TGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGATTCTGATAAGAAG
  AAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAAGAAGTTGATCTTC
  AGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGATCATGGAAAAATTGA
  GTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTA
  AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCT
  GGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCCACGCC
  CATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAAACAGGAAGT
  GGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAGGAAGGAG
  AGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAGATTACTAAAGAGTGTAAGAA
  GTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCT
  GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACA
  GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT
  GGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAGACGGTTATGTTTTCA
  GCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTCAGTAAACCTATTGAAGTACAAGTTG
  GAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTT
  GAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA
  CATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTG
  ATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCTACCTC
  TGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGCTGCCCCAACCATTAT
  GAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAACAAGGGTTATGCTTATACTTTTATCA
  CAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCC
  TGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGT
  AGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGT
  TACAAAAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAGCAAAT
  TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCT
  CCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGA
  ATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCT
  GGCTCCCACTGTTTCTGCAAAAACCATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTAT
  GTGCCGTTAGAGAAACAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAG
  AAGAATTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAG
  AATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAG
  GAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAG
  AAATCACCAGGCTCATAAAAGAAGAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAG
  ATACAAAGTCTTATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014829 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGATGGCCGCCAC
  AGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGGGCAGCTCAGC
  CTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTATTCCCGCGGTC
  GGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCT
  GGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGA
  CGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGGAGAGGTCTCGTAGCAGGGATAAAAG
  AAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAAGTAGAGAGAGAGACAG
  AAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGGAGTAGAAGCC
  GGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAGA
  TTCTAGGTCCAAAGAGAAAACTGATGGTGGNGGGAAAGNNTCTAAGAGAAGAANAAAGAA
  CAAGATGACAAGGAGGATGAAAAAGAANAAGATGCTGGCAACTTTGACCAGAATAAGCTG
  GGAAGAAGAAATGAGAAAGCGAANAGAAAGAGTAGAAAAATGGCGAAGAGAGCAACGTAN
  AAAGGCTATGGAAAACATAGGAGAACTGAAAAAGGAAATCGAAGAGATGAACANGGNAAN
  AAGGGNAGGTTAAAGGACGATGATGATGACAAAAATGATCCTGCAGAAGCTGAAAAGGAA
  GGAAAGAAATGGAGGGTGAGGAGTTAAATCCCTTAAATGCTTACTGGAAAAAGGAAAGAG
  GAATTAAAAATTTTACTGAAAAGTGTAAAGGGGGGGGGGGAAATGAAAAAAATTCTGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014829 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTGTAAGACTTC
  TATTATAACATTCTTAAATCCAGTTCATATTGTTCCAGAGTCATGTAACATCAAGGTGGG
  GACACAAAATATGATAGTGAATTTTACCTTTTCTTAGGAAATGATCACAATTTCATTGAA
  AGTACAGGATAAAGTGAATAGCCCCTTAACGACTTGTGTACTTTATTGTCTTCAATAGAA
  AGTTCAAATATTTCCATTTTTTAAACCTCTAGAATATTATCCGGACATTTAAAATATTGA
  TATTTGAGTTTACATTAAAGGACATCATACTCTGAAAACAGGCTGGCTTTAAATATAGTT
  TGACTGATTGTGGGGGTACTTACGGACAGATTTCTTTACCAAGTACCAGTTTCTTATATT
  TAAAAAAAATCAGCAAGATAGAATTTAAAAAATCTTCATTTACAATACATTGTAAACTGC
  AGACAGCAACTGCCACATACATCATAGACCAGCACAGGTAAAAATCTTTTTTCCGGATGT
  CTATAAGACTTTGTATCTTCCTTTATTTGTTGGTTGGTATGAATTTTGCAGCCGGATCAG
  CTCTTCTTTTATGAGCCTGGTGATTTCTGCCTTTGCTTTCTGCACAGCCAGTTCATTGGC
  ACTTTCAATTGCCAAGTAAATCTTCCGCTCGCCTTCCTTGGGTTCTTTGCCAGGAGGGAA
  GTANGTTCCTCTGATTGTAATTGCGGCTTCAGAGTATTCACTGATTCTCTGCAGAGCTTC
  TTAGAGGTAACTTTCCCCTAGCAGTCTGTGGGAAGNCATTGATCTCTAAATCTTCTCATA
  TCTCTAAAAGATNCATTCTGCCACCATCCTGGCTCTCTTCTTNCTGGTTCTCTACGCACA
  TATTGAGCTGGCATTGATCTTTTCAGCAGTGTCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014829
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014829.2, NP_055644.2
• RefSeq Size: 5695 bp
• RefSeq ORF: 3096 bp
• Locus ID: 9879
• UniProt ID: Q7L014
• Cytogenetics: 5q31.1
• Domains: DEAD, helicase_C
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.