DDX46 (NM_014829) Human Tagged ORF Clone

CAT#: RG202320

DDX46 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)

Product Images

Circular map for RG202320

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDX46
• Synonyms: Prp5; PRPF5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202320 representing NM_014829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGAA
  GTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGATAG
  GAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGT
  TCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAA
  GGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAG
  ATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGATGACAAG
  GAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGAAAGCGAA
  AAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAACTGAAAAA
  GGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCT
  GCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAG
  AAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAGAAGTC
  TGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGATTCTGATAAGAAG
  AAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAAGAAGTTGATCTTC
  AGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGATCATGGAAAAATTGA
  GTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTA
  AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCT
  GGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCCACGCC
  CATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAAACAGGAAGT
  GGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAGGAAGGAG
  AGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAGATTACTAAAGAGTGTAAGAA
  GTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCT
  GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACA
  GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT
  GGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAGACGGTTATGTTTTCA
  GCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTCAGTAAACCTATTGAAGTACAAGTTG
  GAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTT
  GAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA
  CATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTG
  ATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCTACCTC
  TGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGCTGCCCCAACCATTAT
  GAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAACAAGGGTTATGCTTATACTTTTATCA
  CAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCC
  TGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGT
  AGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGT
  TACAAAAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAGCAAAT
  TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCT
  CCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGA
  ATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCT
  GGCTCCCACTGTTTCTGCAAAAACCATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTAT
  GTGCCGTTAGAGAAACAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAG
  AAGAATTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAG
  AATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAG
  GAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAG
  AAATCACCAGGCTCATAAAAGAAGAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAG
  ATACAAAGTCTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202320 representing NM_014829
  Red=Cloning site Green=Tags(s)
  
  MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRR
  SRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDK
  EDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDP
  AEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKK
  KGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEV
  NVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGS
  GKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIA
  ELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFS
  ATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQE
  HADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHY
  EDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKS
  SGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPA
  PTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNY
  VPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPK
  EGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014829
• ORF Size: 3093 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014829.4
• RefSeq Size: 5695 bp
• RefSeq ORF: 3096 bp
• Locus ID: 9879
• UniProt ID: Q7L014
• Cytogenetics: 5q31.1
• Domains: DEAD, helicase_C
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]