METTL9 (NM_016025) Human Untagged Clone

CAT#: SC114501

METTL9 (untagged)-Human methyltransferase like 9 (METTL9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: METTL9
• Synonyms: CGI-81; DREV; DREV1; PAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016025, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGACTGCTGGCGGGCTGGCTGTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAGGATGTGGACGC
  TGCGGAGCCCGCTCACCCGCTCCCTGTACGTGAACATGACTAGCGGCCCGGGTGGGCCGGCGGCGGCCGC
  GGGCGGCAGGAAGGAGAACCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTCCAGGCT
  GTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCGGGCT
  GGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTAT
  CAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGTTTCAGAGACTGCTTAAAATT
  AATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACAAAAATCATGA
  GCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAA
  ATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTCCAGTATGATGTCATCAGCTGCCTGAAC
  TTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAGTGTCTTGGAGCCAACTAGAG
  GCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAACGTAGGTGGCAAGTGGGAGAA
  ACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAAGAACAAGTGAATAGTCTGCCTGAAGTTTTC
  AGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTACCATACCTGTGTGAAGGCGACATGTATAATG
  ACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAACCAGTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016025 unedited
  GGTGTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCCTGGC
  GTCCGTGTGGCTGGCGCGNGAGATGTGGACGCTGCGGAGCCCGCTCACCCGCTCCCTGTA
  CGTGAACATGACTAGCGGCCCGGGTGGGCCGGCGGCGGCCGCGGGCGGCAGGAAGGAGAA
  CCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTCCAGGCTGTCTTTGT
  TCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCGGG
  CTGGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTGTTTATGTC
  TAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCA
  GTTTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGG
  TGCTGGAGATGGAGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCAC
  TGAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTAT
  AAATGAATGGCAGAATACGGGGTTCCAGTATGATGTCATCAGCTGCCTGAACTTGCTGGA
  CCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAGTGTCTTGGAGCCAACTAG
  AGGCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAACGGGTGGCA
  GTGNGAGAAACCATCAGANATTTTGGAAATCAAGGACAGAACTGGNGAGAACAGTGAATA
  GTCTGCCTGAAGTTTTCAGAAAAGCTGGNTTTTGTATCGAAGCTTTCACAGACTACATAC
  CTGTGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016025 unedited
  ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATCTCCAAAAAGTTTATTAGCAT
  GATTAAAAACATTTTCTTGAATAATAACAGAAAGAATTTCTAAACATGAAAGAATATCAG
  TGGCTATACCTTAAAACCAGTATTTGGAATTGAATGGACAGCTTTATTGCCAGTCTTCAA
  AAAACAATCCATTGATTGGTTCCATGAAGTCGTGACTATTGCTTTGTCTGGGAATGAGTC
  CCTACTGCAATTAAGTATAAAAAATATCTTCCATCATAATTGGAGTGTGACCTGCATTGG
  AGAGTGGCTCTGAGTAAAGAGTTGATAAAAATCCACAAAGAGTTGGGTTTTTGTCTTTTA
  AAAACATTCCTTTACACACTACAAAGAAATAATTAGTATTTTGGCTTTTTAAATTCCTAC
  ATGATATTTAGAATGGCGGTCCCCAAGGGTCCTCCCTTCACGTAATTGTGAACACAGACC
  CTCTTCCAAGGGCACATCCCGGAGGGTGCGGACTCTGAAGACTTCGACCTCCACGTGTTT
  ATACTGGTTTGAGAACAAAGACAGCGTCATCCAGAACGTAGTAGTCATTATACATGTCGC
  CTTCCACAGGTATGGTAGTCTGGTGAAAGCTTCCATAACAAAACCAGCTTTTCTGAAAAC
  TTCAAGCAGACTATTCACTTGTTCTTCCAGTTCTGTCCTTTGATTTCCAAATTTCTGATG
  GTTTCTCCCACTGGCCACCGTTTTCACATAGGGATGAAAGGNGAGACANGGCCAGGATGA
  CCCTGCCTCTANNTAGCTCCAAGACACTTCTGATATCTNTAACAAATCAAGGGCTGATCA
  CAGCGTCCANCAGGTCAAGCAGCTGATGACATATACTGGAACCCCGTTTTTGCCATTCAT
  TATACCAAGACTCTTGATTCTTTTCTGAGCGGCATATCTANTTCAAAAGCCAGTGGCATA
  ATNCTCAATGAGGCCTAGATTTGGGACTCCCATCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016025
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016025.2, NP_057109.2
• RefSeq Size: 3267 bp
• RefSeq ORF: 3267 bp
• Locus ID: 51108
• UniProt ID: Q9H1A3
• Cytogenetics: 16p12.2
• Domains: DREV
• Gene Summary: Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).