VRL1 (TRPV2) (NM_016113) Human Untagged Clone

CAT#: SC114461

TRPV2 (untagged)-Human transient receptor potential cation channel, subfamily V, member 2 (TRPV2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VRL1
• Synonyms: VRL; VRL-1; VRL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114461 sequence for NM_016113 edited (data generated by NextGen Sequencing)
  ATGACCTCACCCTCCAGCTCTCCAGTTTTCAGGTTGGAGACATTAGATGGAGGCCAAGAA
  GATGGCTCTGAGGCGGACAGAGGAAAGCTGGATTTTGGGAGCGGGCTGCCTCCCATGGAG
  TCACAGTTCCAGGGCGAGGACCGGAAATTCGCCCCTCAGATAAGAGTCAACCTCAACTAC
  CGAAAGGGAACAGGTGCCAGTCAGCCGGATCCAAACCGATTTGACCGAGATCGGCTCTTC
  AATGCGGTCTCCCGGGGTGTCCCCGAGGATCTGGCTGGACTTCCAGAGTACCTGAGCAAG
  ACCAGCAAGTACCTCACCGACTCGGAATACACAGAGGGCTCCACAGGTAAGACGTGCCTG
  ATGAAGGCTGTGCTGAACCTTAAGGACGGGGTCAATGCCTGCATTCTGCCACTGCTGCAG
  ATCGACCGGGACTCTGGCAATCCTCAGCCCCTGGTAAATGCCCAGTGCACAGATGACTAT
  TACCGAGGCCACAGCGCTCTGCACATCGCCATTGAGAAGAGGAGTCTGCAGTGTGTGAAG
  CTCCTGGTGGAGAATGGGGCCAATGTGCATGCCCGGGCCTGCGGCCGCTTCTTCCAGAAG
  GGCCAAGGGACTTGCTTTTATTTCGGTGAGCTACCCCTCTCTTTGGCCGCTTGCACCAAG
  CAGTGGGATGTGGTAAGCTACCTCCTGGAGAACCCACACCAGCCCGCCAGCCTGCAGGCC
  ACTGACTCCCAGGGCAACACAGTCCTGCATGCCCTAGTGATGATCTCGGACAACTCAGCT
  GAGAACATTGCACTGGTGACCAGCATGTATGATGGGCTCCTCCAAGCTGGGGCCCGCCTC
  TGCCCTACCGTGCAGCTTGAGGACATCCGCAACCTGCAGGATCTCACGCCTCTGAAGCTG
  GCCGCCAAGGAGGGCAAGATCGAGATTTTCAGGCACATCCTGCAGCGGGAGTTTTCAGGA
  CTGAGCCACCTTTCCCGAAAGTTCACCGAGTGGTGCTATGGGCCTGTCCGGGTGTCGCTG
  TATGACCTGGCTTCTGTGGACAGCTGTGAGGAGAACTCAGTGCTGGAGATCATTGCCTTT
  CATTGCAAGAGCCCGCACCGACACCGAATGGTCGTTTTGGAGCCCCTGAACAAACTGCTG
  CAGGCGAAATGGGATCTGCTCATCCCCAAGTTCTTCTTAAACTTCCTGTGTAATCTGATC
  TACATGTTCATCTTCACCGCTGTTGCCTACCATCAGCCTACCCTGAAGAAGCAGGCCGCC
  CCTCACCTGAAAGCGGAGGTTGGAAACTCCATGCTGCTGACGGGCCACATCCTTATCCTG
  CTAGGGGGGATCTACCTCCTCGTGGGCCAGCTGTGGTACTTCTGGCGGCGCCACGTGTTC
  ATCTGGATCTCGTTCATAGACAGCTACTTTGAAATCCTCTTCCTGTTCCAGGCCCTGCTC
  ACAGTGGTGTCCCAGGTGCTGTGTTTCCTGGCCATCGAGTGGTACCTGCCCCTGCTTGTG
  TCTGCGCTGGTGCTGGGCTGGCTGAACCTGCTTTACTATACACGTGGCTTCCAGCACACA
  GGCATCTACAGTGTCATGATCCAGAAGGTCATCCTGCGGGACCTGCTGCGCTTCCTTCTG
  ATCTACTTAGTCTTCCTTTTCGGCTTCGCTGTAGCCCTGGTGAGCCTGAGCCAGGAGGCT
  TGGCGCCCCGAAGCTCCTACAGGCCCCAATGCCACAGAGTCAGTGCAGCCCATGGAGGGA
  CAGGAGGACGAGGGCAACGGGGCCCAGTACAGGGGTATCCTGGAAGCCTCCTTGGAGCTC
  TTCAAATTCACCATCGGCATGGGCGAGCTGGCCTTCCAGGAGCAGCTGCACTTCCGCGGC
  ATGGTGCTGCTGCTGCTGCTGGCCTACGTGCTGCTCACCTACATCCTGCTGCTCAACATG
  CTCATCGCCCTCATGAGCGAGACCGTCAACAGTGTCGCCACTGACAGCTGGAGCATCTGG
  AAGCTGCAGAAAGCCATCTCTGTCCTGGAGATGGAGAATGGCTATTGGTGGTGCAGGAAG
  AAGCAGCGGGCAGGTGTGATGCTGACCGTTGGCACTAAGCCAGATGGCAGCCCGGATGAG
  CGCTGGTGCTTCAGGGTGGAGGAGGTGAACTGGGCTTCATGGGAGCAGACGCTGCCTACG
  CTGTGTGAGGACCCGTCAGGGGCAGGTGTCCCTCGAACTCTCGAGAACCCTGTCCTGGCT
  TCCCCTCCCAAGGAGGATGAGGATGGTGCCTCTGAGGAAAACTATGTGCCCGTCCAGCTC
  CTCCAGTCCAACTGA
  
  Clone variation with respect to NM_016113.4
  390 a=>g;427 a=>c;2094 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016113 unedited
  NTGTTCGAATTTTGTATACGACTCACTATAGGGCGGCCGCGCTGGTGGCTAGCCTGTCCT
  GACAGGGGAGAGTTAAGCTCCCGTTCTCCACCGTGCCGGCTGGCCAGGTGGGCTGAGGGT
  GACCGAGAGACCAGAACCTGCTTGCTGGAGCTTAGTGCTCAGAGCTGGGGAGGGAGGTTC
  CGCCGCTCCTCTGCTGTCAGCGCCGGCAGCCCCTCCCGGCTTCACTTCCTCCCGCAGCCC
  CTGCTACTGAGAAGCTCCGGGATCCCAGCAGCCGCCACGCCCTGGCCTCAGCCTGCGGGG
  CTCCAGTCAGGCCAACACCGACGCGCAGCTGGGAGGAAGACAGGACCCTTGACATCTCCA
  TCTGCACAGAGGTCCTGGCTGGACCGAGCAGCCTCCTCCTCCTAGGATGACCTCACCCTC
  CAGCTCTCCAGTTTTCAGGTTGGAGACATTAGATGGAGGCCAAGAAGATGGCTCTGAGGC
  GGACAGAGGAAAGCTGGATTTTGGGAGCGGGCTGCCTCCCATGGAGTCACAGTTCCAGGG
  CGAGGACCGGAAATTCGCCCCTCAGATAAGAGTCAACCTCAACTACCGAAAGGGAACAGG
  TGCCAGTCAGCCGGATCCAAACCGATTTGACCGAGATCGGCTCTTCAATGCGGTCTCCCG
  GGGTGTCCCCGAGGATCTGGCTGGACTTCCAGAGTACCTGAGCAAGACCAGCAAGTACCT
  CACCGACTCGGAATACACAGAGGGCTCCACAGGTAAGACGTGCCTGNATGAAGCTGTGCT
  GAACCTTAAGGACGGNGTCAATGCCTGCATTCTGCCACTGCTGCAGATCGACCGGGACCT
  CTGGCATCCTCAGCCCCTGGTGAATGCCCAGTGCACAGATGACTATTACCGNAGCCACAG
  CGCTCTGCACATCGCCATTGAGAAAGAGAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016113 unedited
  CCGCTTTTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTGAGGGAAGGAAAATATATATTTGCCACCAAAATTTACTGGGACCCCAAAGCCAGC
  AAATGGGGGTGGAAAAAACCTTTGGTTTGTCCTTTGGCCTCCTGCTGGATTTGGGCCATT
  AATTGGACTGGAGGAACTGGACGGGCACATAATTTTCCTCAAAGGCACCATCCTCATCCT
  CCTTGGGAGGGGAAACCCGGACAGGGTTCTCGAAAGTTTGAGGGACACCTGCCCCTGACG
  GGTCCTCACACAGGGTAAGCAGCGTTTGCTCCCATGAAGCCCAATTCACCTCCTCCACCC
  TGAAGCACCAACGCTTATCCGGGCTGCCATTTGGGTTAATGCCAACGGGCAATATCACAC
  CCGGCCGCTGGTTTTTTCTGCACCACCAATAGCCATTCTCCCTCTCCACGACAGAAATGG
  CTTTTCTGCAACTTCCAAATGCCTAAACTGCCAAGGGCGACACTGTTGACCGGTCCCCTT
  CTGAAGGCGAAGAACATGTTCAACCACCAGATGTTTGGGAGCCTATCGTTTGCCACCAAC
  TACTCTTTATCCCTGCCCCGGAAATGCCATGCTCCTGGAAGCCCCCTCGCCCTGCCCATG
  GGAATTTCAAACCCCCAGGAGGTTTCCGAATCCCCTGTCTGGGCTCCGTGCCCTAAACAT
  TCTGACCCCCATGGCGCACTGACTTCGGGGCATGGGGCCTGTAGACTTTCGGGCCCCAAC
  TTTCTTGTTAGGCTCCCCGGCTATCTGAACCCTAAGAAATTTCTCTCCCAAAAAACCTCC
  TGTCTCCCAGAAACCTTTGGACCTACACCTGAAACCCGGGGCCGGAACCCCTCCTTATAT
  AAGTTTTTCTCTCACCCCGCCAAACACACCGGTGGATCCCTCTAGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016113
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016113.3, NP_057197.2
• RefSeq Size: 2797 bp
• RefSeq ORF: 2295 bp
• Locus ID: 51393
• UniProt ID: Q9Y5S1
• Cytogenetics: 17p11.2
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq, Jul 2008]