DDX27 (NM_017895) Human Untagged Clone

CAT#: SC113877

DDX27 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX27
• Synonyms: dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241; RHLP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017895, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTACTTGCGCAAAGACGACGAGGAGGCTGCGAAAAGTTAAGGGCCGGACCGCAGGCTGTGCTCGCTT
  CCGGAAGTGGCTTCTGCGACAACATGCTTGCGGACCTCGGCTTAATCGGAACCATAGGCGAGGATGACGA
  GGTGCCGGTGGAGCCCGAGTCTGACTCCGGGGACGAGGAAGAGGAGGGGCCCATTGTGCTGGGCAGACGA
  CAAAAAGCTTTGGGGAAGAACCGCAGTGCTGATTTCAACCCTGATTTCGTTTTCACTGAGAAGGAGGGGA
  CGTACGATGGCAGCTGGGCCCTGGCTGATGTCATGAGCCAACTCAAGAAGAAGAGGGCAGCCACTACATT
  AGATGAGAAGATTGAGAAAGTTCGAAAGAAAAGGAAAACAGAGGATAAAGAAGCCAAGTCTGGGAAGTTG
  GAAAAGGAGAAAGAAGCAAAGGAAGGCTCTGAACCAAAGGAGCAGGAAGACCTTCAAGAGAATGATGAGG
  AAGGCTCAGAAGATGAAGCCTCGGAGACTGACTACTCATCAGCTGATGAGAACATCCTCACCAAAGCAGA
  TACACTCAAAGTAAAGGATCGGAAGAAGAAGAAGAAGAAAGGACAGGAAGCAGGAGGATTTTTTGAAGAT
  GCATCTCAGTACGATGAAAACCTCTCGTTCCAGGACATGAACCTTTCCCGCCCTCTTCTGAAGGCCATTA
  CAGCCATGGGCTTCAAGCAGCCCACCCCGATCCAGAAGGCGTGCATACCTGTGGGTCTATTGGGGAAGGA
  CATCTGTGCCTGTGCAGCCACTGGGACAGGTAAAACTGCCGCCTTTGCCCTGCCTGTTTTGGAGCGTCTG
  ATTTATAAACCCCGCCAGGCTCCAGTCACCCGCGTGCTGGTGCTAGTGCCCACCCGAGAGCTGGGCATCC
  AGGTGCACTCTGTCACCAGACAGCTGGCCCAGTTCTGCAACATCACCACCTGCCTGGCTGTGGGCGGCTT
  GGATGTGAAGTCTCAGGAAGCAGCTCTTCGGGCAGCGCCTGACATCCTCATCGCCACCCCAGGCCGGCTC
  ATCGATCACCTCCACAACTGCCCTTCCTTCCACCTGAGCAGCATCGAGGTGCTCATCCTGGACGAGGCTG
  ACAGGATGCTGGATGAGTACTTTGAGGAGCAGATGAAGGAGATCATCCGAATGTGTTCCCACCACCGCCA
  GACCATGCTCTTCTCGGCCACCATGACAGACGAGGTGAAAGATCTGGCTTCTGTCTCCTTGAAGAATCCT
  GTCCGGATATTTGTGAACAGCAACACAGATGTGGCTCCCTTCCTGCGGCAGGAGTTCATCCGGATCCGGC
  CTAATCGTGAAGGAGACCGGGAAGCCATCGTGGCAGCTTTGTTGACGAGGACCTTCACTGACCATGTGAT
  GCTGTTCACGCAAACCAAGAAGCAGGCCCACCGCATGCACATCCTCCTGGGGCTCATGGGGCTGCAGGTG
  GGTGAGCTCCATGGCAACTTGTCACAGACGCAGCGGCTGGAGGCCCTCCGGCGTTTTAAGGATGAACAGA
  TTGACATCCTCGTGGCCACTGATGTGGCAGCCCGTGGACTTGACATTGAGGGGGTCAAAACGGTAATCAA
  CTTCACAATGCCTAATACCATCAAACATTATGTCCACCGGGTGGGGCGAACAGCACGTGCTGGCAGGGCT
  GGGCGCTCAGTCTCTCTGGTGGGAGAAGATGAGCGGAAGATGCTGAAGGAGATTGTAAAAGCTGCCAAGG
  CCCCTGTGAAGGCCAGGATACTTCCCCAAGATGTCATCCTCAAATTCCGGGACAAGATTGAGAAAATGGA
  GAAAGATGTGTATGCAGTTCTGCAGCTAGAGGCGGAGGAAAAAGAGATGCAGCAGTCAGAAGCCCAGATC
  AATACAGCAAAGCGGCTCCTGGAGAAGGGGAAGGAGGCAGTGGTCCAAGAGCCCGAGAGGAGCTGGTTCC
  AGACCAAAGAAGAGAGGAAGAAGGAGAAAATTGCCAAAGCTCTGCAGGAATTTGACTTGGCCTTAAGAGG
  AAAGAAGAAAAGGAAGAAGTTTATGAAGGATGCCAAAAAAAAGGGGGAGATGACAGCAGAGGAAAGGTCT
  CAGTTTGAAATCCTCAAGGCGCAGATGTTTGCTGAACGGCTAGCGAAGAGGAATCGCAGAGCCAAGCGGG
  CCCGAGCAATGCCCGAGGAGGAGCCAGTGAGAGGTCCTGCCAAGAAGCAAAAGCAGGGGAAGAAATCTGT
  ATTTGATGAAGAACTCACCAACACAAGCAAGAAGGCCCTGAAACAGTATCGAGCTGGCCCTTCCTTTGAA
  GAAAGGAAACAGTTGGGCTTGCCCCACCAGAGACGAGGAGGAAACTTTAAATCTAAATCCAGATACAAGA
  GGAGGAAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017895 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCTGCCTGTTTT
  GGAGCGTCTGATTTATAAACCCCGCCAGGCTCCAGTCACCCGCGTGCTGGTGCTAGTGCC
  CACCCGAGAGCTGGGCATCCAGGTGCACTCTGTCACCAGACAGCTGGCCCAGTTCTGCAA
  CATCACCACCTGCCTGGCTGTGGGCGGCTTGGATGTGAAGTCTCAGGAAGCAGCTCTTCG
  GGCAGCGCCTGACATCCTCATCGCCACCCCAGGCCGGCTCATCGATCACCTCCACAACTG
  CCCTTCCTTCCACCTGAGCAGCATCGAGGTGCTCATCCTGGACGAGGCTGACAGGATGCT
  GGATGAGTACTTTGAGGAGCAGATGAAGGAGATCATCCGAATGTGTTCCCACCACCGCCA
  GACCATGCTCTTCTCGGCCACCATGACAGACGAGGTGAAAGATCTGGCTTCTGTCTCCTT
  GAAGAATCCTGTCCGGATATTTGTGAACAGCAACACAGATGTGGCTCCCTTTCTGCGGCA
  GGAGTTCATCCGGATCCAGCCTAATCGTGAAGGAGACCGGGAAGCCATCGTGGCAGCTTT
  GTTGACGAGGACCTTCACTGACCATGTGATGCTGTTCACGCAAACCAAGAAGCAGGCCCA
  CCGCATGCACATCCTCCTGGGGCTCATGGGGCTGCAGGTGGGTGAGCTCCATGGCAACTT
  GTCACAGACGCAGCGGCCTGGAGCCCTCCGGGCGTTTAAGATGAACAGATTGACATCCTC
  GTGGCCACTGATGTGGCAGCCCGTGGACTTGACATTNGAGGGGTCAAAACGGTNATCAAC
  TCACAATGCCTAATACCTCAAACTTATGTCCACCGGTGGNGCGAACAGACGTGCTGGCAG
  GNCTGGCGCTCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017895 unedited
  GCGACAGCNTTTAGGGTCGAGAAAAAATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTCTTTCCCCAAAAATACTTCCATTTAATATAGCCATATGAAAGCTAATCACCAAA
  AAGGGAAATGGACAGACATGCTTAGGAAAATAGCTACGTACCATACCACCACACCAAAGG
  GGGGTTTTTGGTTTTGGTTTTTTTTTTTTTTTTGGGAAAAAAGACAGACCAGCCAGGATG
  ACTTCCCACAGGGAAGGGATTAAAGGGCTGCCCCCATGAATTTTTTAAGGCCACAACAGC
  TACTTCCTCCTCTGGGATCGGGATTAAAATTAAAAGTTTCCTCCTCGTCTTTGGGGGGGC
  AAGCCCAACTGTTTCCTTTTTTCAAAGGAAGGGCCAGCTCGATACTGTTTCAGGGCCTTC
  TTGCTTGGGTGGGGGAGTTCTTCATCAAAAACAGATTTCTTCCCCTGCTTTTGCTTCTTG
  GCAGGACCTCTCACTGGCTCCTCCTCGGGCATTGCTCGGGCCCGCTTGGCTCTGCAATTC
  CTCTTCGCTAGCCGTTCAGCAAACATCTGCGCCTTGAGGATTTAAAACTGAGACCTTTCC
  TCTGCTGACATCTCCCCCTTTTTTTTGGCATCCTTAATAAAACTCCTCCTTTTCTTCTTT
  CCTCTTAAGGCCAAGTCAAATTCCTGCAAAGCTTTGGGCAATTTCCTCCTTCTTCCTCTC
  TCCTTGGGCCAGAAACCAGTTCCTCTCGGCCTCTTGGACCACTGCCTTCCTTCCCTTTTC
  CAGAAACCGCTTTGCGGAATGAACCGGGGCTTTGACTGCTGAATTCTTTTTCCTCCGCCT
  TTAGCTGAAAAATGGAAACCATCTTTCCCAATTTTCTAAACTGGCCCGAATTTGAGGAGA
  CACCTGGGGAAAACCGCCCTCAAAGGGCCTGGGAGTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017895
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017895.5, NP_060365.5
• RefSeq Size: 2711 bp
• RefSeq ORF: 1221 bp
• Locus ID: 55661
• UniProt ID: Q96GQ7
• Cytogenetics: 20q13.13
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.