MBNL1 (NM_021038) Human Untagged Clone

CAT#: SC113012

MBNL1 (untagged)-Human muscleblind-like (Drosophila) (MBNL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBNL1
• Synonyms: EXP; MBNL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021038, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGA
  GGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAA
  TGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCAT
  CCACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGG
  CCATGTTGGCCCAGCAAATGCAACTAGCCAATGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAAT
  GTTTTCAGTTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTGGGACCTGTT
  TCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGCACCAATGTTGGTTACAGGGAATCCGGGTGTCC
  CTGTACCTGCAGCTGCTGCAGCTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGAGA
  GTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCTGCTGACAGCACAATG
  ATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGT
  GCAAATACTTTCATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGC
  TGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTACCA
  AAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAAC
  AGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGAC
  ACCCGCTACAAGTGTTGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCT
  GCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACATCTGA
  CTAGCCACAAGTATGTTACCCAGATGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021038 unedited
  NGGTCAAATTGATACACTATTAGGCGCCGGATTCGCCGATCTGCCGGAAATAAGAGGAAA
  AAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTACTTCTGTCTATGCCAAACTAATCA
  ATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGA
  CAACTTTGCTGAAAGCAGCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCT
  TGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGATACC
  AGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAAC
  CTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAGTGGGAGATCTTTTCCTTGATATT
  GACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGA
  CATTCTCATCATCAGTTCAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGG
  GTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAGTGT
  CACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGG
  GACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGT
  TGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAA
  CTGCAAATATCTTCATCCACCCCCACATTTAANAACGCAGTTGGAGATAAATGGACGCCA
  TAACTTGATTCAGCAGAAGACATGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021038 unedited
  GGGGGGGTTGAAATTTTAAACATGCATAGTTTTGAAAAAGTAAGGAATATCTGATCCATG
  ATTTCACCTTAATGGTAAAAGTACCTGCTATTTTTGGTAATGATCTTCAGGCAAATTTGG
  CCCCATGGAGTTTTTGTCAGTCATGAGGGTTTGAAAGGATGGCGTATCTTTCCAGCTGAA
  AAAAATCCTGGCAAACTACAAGCTGTCCACAAAAAAGCAAAGCAACATGTTTGAAGGGGT
  ATGGAGTGCTAACTCTGTGCCACTTTTGCAAGCAATTTTGCTCAGTCTGTCATTGTATTA
  CCCTTAACAAAACTCCTTGTAATTATAGACGTTTTTATTCAAAATAAAATCTTACTATTA
  TACAGGTTTCTCTCTTTTGACTATATACAGAAGTGCAAAAAGTCAACCTTCTCCCTAAAC
  GTTCCAACATGCTAAATTGCAGCATAATCAACCCTCAAGAGTTTGCACACACGCTATAAT
  TTTCATTACGTAAACTTTGAGTATTTGGATTATCAACAAAAAGTCCCTTGTTCTATTTAT
  TGATTATGCAGCTTATTTATAACAAGGCCTGATACTCAGGGATTTCAAAAATATTAAACA
  ATATTTTAACATTTGAAATGGATACAGTAAAAAATAAATTTTATTCTAATATCTAGGATC
  TAGATCTTCTTATAATAACTAATTACAAACAAAATAAATCATCAAAATATTACTCAAATT
  GTCTGCCAGGAATTGGNNGCGATAGCAACCAACCTTAACTTCTTACTTNAGCAATGATTA
  ATATATTNTATAAAGATATCCATATAAAAGAACCTTTTGTTCCTTTTAATGAAACCTACA
  CAAGAACTAACAGGGACTGGCATNCTTTAACCTGAAATATAAAATCAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021038
• Insert Size: 4570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021038.3, NP_066368.2
• RefSeq Size: 6404 bp
• RefSeq ORF: 1149 bp
• Locus ID: 4154
• UniProt ID: Q9NR56
• Cytogenetics: 3q25.1-q25.2
• Domains: zf-CCCH
• Gene Summary: This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes isoform a. Other names for this isoform are EXP41 and MBNL1subscript41.