NFYA (NM_021705) Human Untagged Clone

CAT#: SC112917

NFYA (untagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFYA
• Synonyms: CBF-A; CBF-B; HAP2; NF-YA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021705, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGC
  AGCAGGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC
  CATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAG
  GGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGC
  AGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGAC
  TCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTC
  TATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTA
  TCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG
  GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATG
  GTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTTGAAGAAG
  AGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAAGCCCGAGCTAAACTAGA
  GGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCA
  CGGAAGCGTGGTGAAGGTGGACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAA
  ACCAAGCCGATGAAGAAGCAATGACACAGATCATCCGAGTGTCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021705 unedited
  NGGGTTTCANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTAGG
  ATCTCCAGAGTGGACAGGAATCTCACTTGGAGGGACCATGGAGCAGTATACAGCAAACAG
  CAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGCAGCAGGTCCAAGG
  GCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCAT
  CATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGG
  AACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGG
  ACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCC
  CCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACA
  GCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGG
  CACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAG
  TTTGGCAGGAGCACAGATTGTTCANACAGGAGCCAATACCAACACAACCAGCAGTGGGCA
  AGGGACTGTCACTGTGACACTACCAGTGGCANGGCATGTGGTCAATTCAGGAAGGATGGT
  CATGATGGTTCCTGGGGCTGCCTCTGTGCCTGCTANCCAAAGATCCCTCTACTGGAGCAN
  AGATGCTGAAGAAGACCTCTTACGTGAATGCCAACATACCACGTATTCTTAAGAGGAGCA
  GCCCGAGCTAACTAGAGCAGAGGGGAAATTCAAGGAGAGAAGAATACTGCTGAGTCTCGC
  ACGTCTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021705 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTATTNTTATAAATTTAT
  TTTAAAAAATAACAATTGCAATGACATTAATGATCGATCACCTGTACGCCCCCATACTGC
  TGGGGGAAAAACCTTCGATATAGTTGAATCCCAAAGAAAAATCAAGACATTATTATCAGG
  CACCCTATAGCCACAAAATGCTCTGTTGGTCCAGTGGCATCGACACTGACATGTTCAATG
  TACCAGTGCAGTTAAAATATATACACTTAAAATTATCCTGCAAAAAAAAAAAAAAGCACT
  CAAGTTGCATAACTAGGCATTAAACCAATTATTCATTCATCCATTCACTCTTTCTCCATC
  TGTAAAGTTATCACTACAGTCACAAATAAAAATGAATAAAAGAACTAGTATCAAAGAAAA
  AATCCAAATGTCAATACCCTGCCCCCACTTTGTGGGCTACTATGGTGTGCCAAAGTGACC
  ACAAGAAAAGGGTCATGAGCTATTTGAAATGCAATGACAAAATGACAACTGAAGCATTTC
  AAACAAAAATTAAAGTCATCTCCCCCCAAATAAAAAAATCAGCACCAAGGCTGGCATTAC
  TATGTACNAACTTACAGACACAAATAATAGCATCTTTTTACTGTTGGCAGCTTAAGCACT
  CAAGTTAGGTCTGAGGAATTTTTTTTGACCTCTGCATCTAAACTCCAAATTACCCAAGGA
  TATAATTCCCCCATGGTAGAAATCTATTTCCTGAAAACTTGGGACTACTGAAAACATTAT
  ATCCCAACTCTTGGAAAGTAGAGGTTCTTATGAGCTACAAAGGAAAGTCCTTGCATCTGG
  AGCCAGACGATACCAGAACCGGACTAGAAAATTACCTCCCGTCCCTTGAAAACCGCCCTT
  TTTTTAAATTAACAATTTTTTGGGGGTTTAAAGGCTAATTTTGCTAAACGGTACCGAGCA
  AAAAACATTTTTTGGGTTACCCCAGTCCCGAAAAGGTTTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021705
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021705.2, NP_068351.1
• RefSeq Size: 2747 bp
• RefSeq ORF: 957 bp
• Locus ID: 4800
• UniProt ID: P23511
• Cytogenetics: 6p21.1
• Domains: CBF
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation
• Gene Summary: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) is missing alternate exon B, resulting in the short isoform, isoform 2.