NFYA (NM_021705) Human Tagged ORF Clone

CAT#: RC205981

NFYA (Myc-DDK-tagged)-Human nuclear transcription factor Y, alpha (NFYA), transcript variant 2

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_021705" in other vectors (4)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit Polyclonal anti-NFYA antibody
    • 100 ul

USD 539.00

Other products for "NFYA"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NFYA
Synonyms CBF-A; CBF-B; HAP2; NF-YA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205981 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGACAGATTCAGC
AGCAGGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC
CATCATGGTCCAGGCTGTCCCTGGTGGGCAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAG
GGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGC
AGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGAC
TCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTC
TATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTA
TCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG
GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATG
GTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTTGAAGAAG
AGCCTCTCTACGTGAATGCCAAACAATACAACCGTATTCTTAAGAGGAGGCAAGCCCGAGCTAAACTAGA
GGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCA
CGGAAGCGTGGTGAAGGTGGACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAA
ACCAAGCCGATGAAGAAGCAATGACACAGATCATCCGAGTGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205981 protein sequence
Red=Cloning site Green=Tags(s)

MEQYTANSNSSTEQIVVQAGQIQQQVQGQPLMVQVSGGQLITSTGQPIMVQAVPGGQGQTIMQVPVSGTQ
GLQQIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTAVTAGQTQTQQQIAVQGQQVAQTAEGQTIV
YQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGANTNTTSSGQGTVTVTLPVAGNVVNSGGMVMM
VPGAGSVPAIQRIPLPGAEMLEEEPLYVNAKQYNRILKRRQARAKLEAEGKIPKERRKYLHESRHRHAMA
RKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021705
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021705.4
RefSeq Size 6149 bp
RefSeq ORF 957 bp
Locus ID 4800
UniProt ID P23511
Cytogenetics 6p21.1
Domains CBF
Protein Families Transcription Factors
Protein Pathways Antigen processing and presentation
MW 33.9 kDa
Gene Summary The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

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