Optineurin (OPTN) (NM_021980) Human Untagged Clone

CAT#: SC112844

OPTN (untagged)-Human optineurin (OPTN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Optineurin
• Synonyms: ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; TFIIIA-INTP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021980 edited
  GAATTCGGCACGAGGCTCCGCCACCGCCGCCGCCCGCCGGCAGGTTCCCTGGTCAGCGTC
  CCATCCCGGTCGGGAGTTCTCTCCAGGCGGCACGATGCCGAGGAAACAGTGACCCTGAGC
  GAAGCCAAGCCGGGCGGCAGGTGTGGCTTTGATAGCTGGTGGTGCCACTTCCTGGCCTTG
  GATGAGCCGTACGCCTCTGTAAACCCAACTTCCTCACCTTTGAAACAGCTGCCTGGTTCA
  GCATTAATGAAGATTAGTCAGTGACAGGCCTGGTGTGCTGAGTCCGCACATAGAACTTCT
  GCAATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGC
  ACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCCCGGAGGAG
  CTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAGCTGAAAGAAGCCATGAAG
  CTAAATAATCAAGCCATGAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAG
  AAGGAAGAACGCCAGTTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGC
  CTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGA
  AAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGGAGCAGGA
  AAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAGAAGGCAGACCTGTTGGG
  CATCGTGTCTGAACTGCAGCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGT
  TGAAATTAGGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCC
  TGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGC
  AGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCCTGCTGTG
  CCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCACTCAAGGAGGCCAAAGA
  AAGAGTTTCAGATTTTGAAAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGA
  GGGGAGCACAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGT
  GGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAA
  ACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCCTTGAAAG
  GAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAGCTTGTTTATACTAACAA
  AAAGTTAGAGCTACAAGTGGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAAC
  AGAGGATGAAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGA
  ACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGA
  CAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTCTGGCTTC
  CAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAGCAGGAAGAGGACCTGGA
  AACCATGACCATCCTCAGGGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAG
  AGCAGCGAGAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCT
  GCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAG
  TCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAAGAGGAGC
  TGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCATTCCTGCCCCAAGTGTGG
  AGAGGTTCTGCCTGACATAGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGT
  GTTGATGTATCACCTCCCCAAAACTGTTGGTAAATGTCAGATTTTTTCCTCCAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021980 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCGCCACCGCCG
  CCGCCCGCCGGCAGGTTCCCTGGTCAGCGTCCCATCCCGGTCGGGAGTTCTCTCCAGGCG
  GCACGATGCCGAGGAAACAGTGACCCTGAGCGAAGCCAAGCCGGGCGGCAGGTGTGGCTT
  TGATAGCTGGTGGTGCCACTTCCTGGCCTTGGATGAGCCGTACGCCTCTGTAAACCCAAC
  TTCCTCACCTTTGAAACAGCTGCCTGGTTCAGCATTAATGAAGATTAGTCAGTGACAGGC
  CTGGTGTGCTGAGTCCGCACATAGAACTTCTGCAATGTCCCATCAACCTCTCAGCTGCCT
  CACTGAAAAGGAGGACAGCCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCA
  CCCAAACCTGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGAC
  CGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATT
  TGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTTGAGATAC
  AGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAGAATGAGAAATTGAAGG
  AAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACT
  CCAGNCTTNCCAGGGCCGAAGCGGAGCAGGANAAGGACCCAGCTCAGACCCANNGTGGTG
  AGGGCTACAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTG
  AACTTCANCGGGCTCTCAGAAGATTCCTTTGNTGAATTANGATGGCTGAAAGAGAACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021980 unedited
  TTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTGGGAGGAAAAAACTGACATTTACCAACAGTTTTGGGGAGGGGATACCTC
  AACACTTAAATGATGCCATCCATCACGTGAATCTGTAACGGGTCTATGTCAGGCAAAACC
  TCTCCACACTTGGGGCAGGAATGAATCGGAATATTCCGCTGTTGCCGCCAGTCCCTGGCC
  TCAACTCCTTTTTGAACAAGGGAAACCTGCTGGGCAAAATCACTTGTTCTCGCCCCATGA
  CGACTTTGCATTTCCATCAAGGACTGGCTGCCTCCGTCTTTGAAAGCATCATTTTTTTTT
  AACAAAACTGCCAACTGCAATGCCAATTGCTCCTTTTCCTCATGAAATTTCTCTCTCGCT
  GCTCTTTCAGCATGAAAATCAAAACAGTAAACTTCCATTTGAACCCTGAGGAAGGGCATG
  GGTTCCAAGGCCTCTTCCTGCTTGGCAAAGGGTTGCTTCATTTCATCCATTTGCAGCTGT
  TTGGAAACCAAAACCTTTTCTGGCAGTTCCAGTTTTTCACTCAGTTCCTTTAACACTGGC
  CTGGCCACTTTTTCTGACTCTTTTCTTGGTAATTCCTCAATTGGTTTCAAAGCATTATTA
  TGTTCTTGAAGAAACTTGGTGTGTGTCATCTGTAACCAGTTAATTTGGACTTTTCATCCT
  CTGGTTTACCCCGTTCCATTTTGAATTCTGATAGCAAGCTTTCCACATTGGAGCTCTAAC
  TTTTTGTTAATATAAAACAAGCTCTTGCTTTTCATTCAACTCTGAAGGAATTGGGGAAAT
  TTTCCCTTCAAGGGGCCGACACCTTTNCTTGGAGGCTCTTCCTCATTAACTCAACTTCCC
  TGAGATTTGATGAGCCTCTTGGACCTCCTTAACAAGATGCACCTGAAGGTAAGGCTTTCA
  CTTCCTTCACAATTTCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021980
• Insert Size: 2140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021980.4, NP_068815.2
• RefSeq Size: 3376 bp
• RefSeq ORF: 1734 bp
• Locus ID: 10133
• UniProt ID: Q96CV9
• Cytogenetics: 10p13
• Domains: zf-C2H2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.