HRPT2 (CDC73) (NM_024529) Human Untagged Clone

CAT#: SC112162

CDC73 (untagged)-Human cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CDC73)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HRPT2
• Synonyms: C1orf28; FIHP; HPTJT; HRPT1; HRPT2; HYX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112162 sequence for NM_024529 edited (data generated by NextGen Sequencing)
  ATGGCGGACGTGCTTAGCGTCCTGCGACAGTACAACATCCAGAAGAAGGAGATTGTGGTG
  AAGGGAGACGAAGTGATCTTCGGGGAGTTCTCCTGGCCCAAGAATGTGAAGACCAACTAT
  GTTGTTTGGGGGACTGGAAAGGAAGGCCAACCCAGAGAGTACTACACATTGGATTCCATT
  TTATTTCTACTTAATAACGTGCACCTTTCTCATCCTGTTTATGTCCGACGTGCAGCTACT
  GAAAATATTCCTGTGGTTAGAAGACCTGATCGAAAAGATCTACTTGGATATCTCAATGGT
  GAAGCGTCAACATCGGCAAGTATAGACAGAAGCGCTCCCTTAGAAATAGGTCTTCAGCGA
  TCTACTCAAGTCAAACGAGCTGCAGATGAAGTTTTAGCAGAAGCAAAGAAACCACGAATT
  GAGGATGAAGAGTGTGTGCGCCTTGATAAAGAGAGATTGGCTGCCCGTTTGGAGGGTCAC
  AAAGAAGGGATTGTACAGACTGAACAGATTAGGTCTTTGTCTGAAGCTATGTCAGTGGAA
  AAAATTGCTGCAATCAAAGCCAAAATTATGGCTAAGAAAAGATCTACTATCAAGACTGAT
  CTAGATGATGACATAACTGCCCTTAAACAGAGGAGTTTTGTGGATGCTGAGGTAGATGTG
  ACCCGAGATATTGTCAGCAGAGAGAGAGTATGGAGGACACGAACAACTATCTTACAAAGC
  ACAGGAAAGAATTTTTCCAAGAACATTTTTGCAATTCTTCAATCTGTAAAAGCCAGAGAA
  GAAGGGCGTGCACCTGAACAGCGACCTGCCCCAAATGCAGCACCTGTGGATCCCACTTTG
  CGCACCAAACAGCCTATCCCAGCTGCCTATAACAGATACGATCAGGAAAGATTCAAAGGA
  AAAGAAGAAACGGAAGGCTTCAAAATTGACACTATGGGAACCTACCATGGTATGACACTG
  AAATCTGTAACGGAGGGTGCATCTGCCCGGAAGACTCAGACTCCTGCAGCCCAGCCAGTA
  CCAAGACCAGTTTCTCAAGCAAGACCTCCCCCAAATCAGAAGAAAGGATCTCGAACACCC
  ATTATCATAATTCCTGCAGCTACCACCTCTTTAATAACCATGCTTAATGCAAAAGACCTT
  CTACAGGACCTGAAATTTGTCCCATCAGATGAAAAGAAGAAACAAGGTTGTCAACGAGAA
  AATGAAACTCTAATACAAAGAAGAAAAGACCAGATGCAACCAGGGGGCACTGCAATTAGT
  GTTACAGTACCTTATAGAGTAGTAGACCAGCCCCTTAAACTTATGCCTCAAGACTGGGAC
  CGCGTTGTAGCCGTTTTTGTGCAGGGTCCTGCATGGCAGTTCAAAGGTTGGCCATGGCTT
  TTGCCTGATGGATCACCAGTTGATATATTTGCTAAAATTAAAGCCTTCCATCTGAAGTAT
  GATGAAGTTCGTCTGGATCCAAATGTTCAGAAATGGGATGTAACAGTATTAGAACTCAGC
  TATCACAAACGTCATTTGGATAGACCAGTGTTCTTACGGTTTTGGGAAACATTGGACAGG
  TACATGGTAAAGCATAAATCGCACTTGAGATTCTGA
  
  Clone variation with respect to NM_024529.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024529 unedited
  ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCCTGGGTGGCTACTGCCCCTG
  CTGCTGTCGTAGGCGAGGACGGCTGTTAGTGCTGCTGCTGTTGGTTCGTCGCGGCGGCGA
  AGGAGGAGGAGGAAGAGGGCGAGGCGACAAGAGAAGAAGGAGGCAGGCGCGGCGGCAGCG
  GCGGCGCCCCGAGCCGGCGGAGGCGAGGGGGGGGAAGATGGCGGACGTGCTTAGCGTCCT
  GCGACAGTACAACATCCAGAAGAAGGAGATTGTGGTGAAGGGAGACGAAGTGATCTTCGG
  GGAGTTCTCCTGGCCCAAGAATGTGAAGACCAACTATGTTGTTTGGGGGACTGGAAAGGA
  AGGCCAACCCAGAGAGTACTACACATTGGATTCCATTTTATTTCTACTTAATAACGTGCA
  CCTTTCTCATCCTGTTTATGTCCGACGTGCAGCTACTGAAAATATTCCTGTGGTTAGAAG
  ACCTGATCGAAAAGATCTACTTGGATATCTCAATGGTGAAGCGTCAACATCGGCAAGTAT
  AGACAGAAGCGCTCCCTTAGAAATAGGTCTTCAGCGATCTACTCAAGTCAAACGAGCTGC
  AGATGAAGTTNTAGCAGAAGCANAGAAACCACGAATTGAGGATGAAGAGTGTGTGCGCCT
  TGATAAAGAGAGATTGGCTGCCCGTTTGGAGGGTCACAAAGAAGGGATTGTACANGACTG
  ACAGATTANGTCTTTGTCTGAAGCTATGTCAGTGGAAAAAAATGCTGCNATCAAAGCCAN
  AATTATGGCTAAGAAAGATCTACTANTCAGACTGATCTAGATGATGACCTANCTGCCCTT
  AACAGNNAGAGTTTGTGATGCTGAGGTAATGTGACCCGAGATATTGCACCAAGAGAGAGT
  TGGAGGGCCNNNACACTATCTTACAAGCCAGGAAGAATTTTTCCAGAACATTTTTGCATC
  CTTCATTTGTAAAGCCACAGAGAAGGGCGGGCACTGAAAGGGACTGCCCCAATGCAAACT
  GTGGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024529 unedited
  NTTTGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGTT
  AAAATATAGTTTTTATATTTTATACCATATTGAATACAGACATAAAAATACCAGTGTTTT
  TCTCTTGTTTCTTTTCAGACAATTAAGCAAATGTTTTCTTTGGTGCCTGGGAGTTTATCA
  ATATTTAGGGATAATGGAAGATAAGAACTCCAAGACTGACAAGCCTAAAATAGAAGATTC
  AAAATCCTATACCATAAAAATTACAATCAAAAGTGGACTAAAGAGAAAAGGAGCTTAAAG
  TTATACTGCAATAGGGCTGATACATATTCTTCCCCCAAATCATACTCTGGCTCCCAAAAT
  AAAAAATACTACAGCCTATTGTGGTTTTAATCTGTGTTTTGAGATTTTTTTCAAATGGAA
  AGATATTACAGATAGAAACACATCATTCTAGGTTTCAAAATCACCAAAGTCAGTGAGAAG
  AATGTACATTCATTTAAATGTAATCATTTGTAAAAAAAGAAATCTTGAAAAGGCTGAACA
  GTTCAATCAAATTGAAGAATTGATCTAGATTTTTCATTATTCTTTTTAAATAATGAGTAA
  GTGTAGATATAGTGTACATACAAGTTGACATTCTGATTCATTCAAAATAGTCACATCCCT
  TTGCTTGGTTTTGGATGTTCCAGAGACTAAAATTTAATACCATTCATAACATAGTATTTT
  CAGTAAAAAGAATTATAGAGCAATGAANAATCAGAAGATGGCTCAGAGAGCCTTATAATC
  TCTAAATATACACACATTTATAACCTGCATTTATTTTCTAAAGAAACANATTTTAAAGTA
  TGCTAATTTAACCAATGGAGAANNAACTTCTATCANATATACAATTTCCATTACAGACTA
  GAAGGCTAATATAAAAAAATAGTTGTAGCCCAAAAGTTTGCTTATATGGATGACAGGTAT
  CATCTCCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_024529
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024529.3, NP_078805.3
• RefSeq Size: 2980 bp
• RefSeq ORF: 1596 bp
• Locus ID: 79577
• UniProt ID: Q6P1J9
• Cytogenetics: 1q31.2
• Domains: Cdc73
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma. [provided by RefSeq, Jul 2009]