ALDH1L1 (NM_012190) Human Untagged Clone

CAT#: SC111756

ALDH1L1 (untagged)-Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH1L1
• Synonyms: 10-fTHF; 10-FTHFDH; FDH; FTHFD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111756 sequence for NM_012190 edited (data generated by NextGen Sequencing)
  ATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGGAAGTTTACTGCCACCTGAGG
  AAGGAGGGCCACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGGAAAGGCCGAC
  CCCCTGGGTCTGGAAGCTGAGAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGT
  GCAAAAGGACAGGCTTTGCCTGATGTGGTGGCAAAATACCAGGCTTTGGGGGCCGAGCTC
  AACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAGATAATCAGTGCCCCCCGGCAT
  GGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCACCGAGGGGCCTCGGCCATCAAC
  TGGACCCTCATTCACGGAGATAAGAAAGGGGGGTTTTCCATCTTCTGGGCGGATGATGGT
  CTGGACACCGGAGACCTGCTGCTGCAGAAGGAGTGTGAGGTGCTCCCGGACGACACCGTG
  AGCACGCTGTACAACCGCTTCCTCTTCCCTGAAGGCATCAAAGGGATGGTGCAGGCCGTG
  AGGCTGATCGCTGAGGGCAAAGCCCCCAGACTCCCTCAGCCTGAGGAAGGAGCCACCTAT
  GAGGGGATTCAGAAGAAGGAGACAGCCAAGATCAACTGGGACCAGCCGGCAGAGGCCATT
  CACAACTGGATCCGCGGGAACGACAAGGTGCCGGGAGCCTGGACAGAGGCCTGTGAACAG
  AAACTGACATTTTTCAACTCAACGCTGAACACTTCAGGCCTGGTGCCCGAGGGAGACGCT
  TTGCCCATCCCAGGAGCCCATCGGCCAGGGGTGGTCACCAAAGCAGGACTCATCCTCTTT
  GGGAATGATGACAAAATGCTGCTGGTGAAGAATATTCAGCTGGAGGATGGCAAAATGATC
  CTGGCCTCGAACTTCTTTAAGGGGGCAGCCAGCAGTGTCCTTGAGCTGACAGAGGCAGAG
  CTGGTTACTGCGGAGGCTGTGCGGAGTTTTTGGCAGCGGATCCTCCCCAAAGTCCTGGAG
  GTTGAAGACTCCACTGATTTCTTCAAGTCAGGGGCCGCGTCTGTGGACGTTGTGAGGCTG
  GTGGAGGAAGTGAAGGAGCTGTGTGATGGCCTGGAGTTAGAAAATGAAGATGTGTACATG
  GCATCCACCTTTGGGGACTTCATCCAGCTGTTAGTGAGGAAGCTTCGAGGGCACGATGAG
  GAGGGCGAGTGCAGCATTGACTACGTGGAAATGGCAGTGAACAAGCGCACTGTCCGCATG
  CCCCACCAGCTCTTCATTGGGGGGGAGTTCGTGGATGCCGAGGGCGCCAAGACCTCTGAG
  ACCATCAATCCCACCGATGGAAGTGTCATCTGCCAGGTATCCCTGGCCCAAGTCACCGAC
  GTCGACAAGGCAGTGGCCGCAGCCAAGGATGCCTTTGAGAATGGACGGTGGGGGAAGATC
  GGTGCGCGGGACCGGGGCCGGCTGATGTACAGGTTGGCAGATCTCATGGAGCAGCACCAG
  GAGGAGCTGGCCACCATTGAGGCCCTGGATGCGGGTGCCGTCTACACGCTGGCCCTGAAG
  ACCCACGTGGGCATGTCCATCCAGACCTTCCGCTACTTTGCTGGCTGGTGTGACAAGATC
  CAGGGCTCCACCATCCCCATCAACCAGGCCAGACCCAACCGCAACCTGACCTTGACCAGG
  AAGGAGCCTGTTGGGGTTTGTGGCATCATCATCCCCTGGAACTATCCCCTGATGATGCTG
  TCCTGGAAGACAGCTGCCTGCCTGGCTGCCGGGAACACAGTGGTGATCAAGCCTGCTCAG
  GTGACCCCACTCACAGCCTTGAAGTTTGCAGAGCTGACATTAAAGGCCGGCATTCCCAAA
  GGTGTGGTTAACGTCCTCCCAGGATCTGGCTCCCTGGTCGGCCAGAGACTCTCAGACCAT
  CCTGATGTGAGGAAAATCGGGTTCACAGGCTCCACAGAGGTGGGCAAGCACATCATGAAA
  AGCTGTGCCATAAGTAACGTGAAGAAGGTGTCCCTGGAACTGGGCGGGAAGTCACCCCTC
  ATCATCTTTGCTGACTGTGACCTCAACAAGGCTGTGCAGATGGGGATGAGTTCTGTTTTC
  TTCAACAAAGGAGAGAATTGCATTGCAGCAGGCCGACTCTTTGTGGAGGACTCCATTCAT
  GATGAGTTCGTGCGGAGAGTGGTAGAAGAGGTGCGGAAGATGAAGGTGGGCAACCCGCTG
  GACAGGGACACCGACCACGGGCCGCAGAATCACCATGCCCACCTTGTGAAGCTGATGGAG
  TACTGCCAGCATGGCGTGAAGGAAGGGGCCACACTGGTCTGCGGCGGGAATCAGGTCCCT
  CGGCCAGGGTTCTTCTTTGAGCCAACTGTTTTCACAGACGTGGAAGACCACATGTTCATA
  GCCAAGGAGGAGTCCTTCGGGCCTGTCATGATCATCTCTCGGTTTGCTGATGGGGACTTG
  GATGCCGTGCTGTCTCGGGCCAATGCCACGGAATTTGGCCTGGCTTCTGGTGTCTTCACC
  AGGGACATCAACAAGGCCCTGTATGTCAGTGACAAGCTCCAGGCAGGCACTGTGTTTGTC
  AACACGTACAACAAGACCGACGTGGCCGCTCCCTTCGGAGGATTCAAACAGTCTGGATTT
  GGCAAAGATCTAGGAGAGGCGGCTCTGAACGAGTACCTGCGGGTCAAGACAGTGACCTTC
  GAATACTGA
  
  Clone variation with respect to NM_012190.2
  988 g=>t;1185 g=>t;1192 g=>c;1441 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012190 unedited
  TTCGGNCCTCAGCTGCGGCTCATTTAAGTCTAACCTGCCCGGGACGCCCGCACCAACTCC
  TGCGCGCGGTCCTTCCAACCCTCCTGCTACCATGAAGATGCAGTGATTGGACAACCTGTT
  TGGCCAGAATTTACTGCCACCTGAGAAGAGGCCACGAATGTNGGGTAGNTCACTGTTCCA
  TACAAGGATTGAAAGGCCGACCCCCTGGGTCTGGAAGCTGAGAAGGATGGAGTGCCGGTA
  TTCAAGTACTCCCTGTGGCGTGCAAAAGGACAGGCTTTGCCTGATGTGGTGGCAAAATAC
  CAGGCTTTGGGGGCCGAGCTCAACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAG
  ATAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCAC
  CGAGGGGCCTCGGCCATCAACTGGACCCTCATTCACGGAGATAAGAAAGGGGGGTTTTCC
  ATCTTCTGGGCGGATGATGGTCTGGACACCGGAGACCTGCTGCTGCAGAAAGAGTGTGAG
  GTGCTCCCGGACGACACCGTGAGCACGCTGTACAACCGCTTCCTCTTCCCTGAGGCATCA
  AAGGGATGGTGCAGGCCGTGAGGCTGATCCCTGANGCAAAACCCCCAAACTCCCTCACCC
  TGAGGAAGGAACACCTATGAGGGGATTCAGAAAAAGGAGAACGCCAGAATCACTTGGGAC
  CAGCCCGGCAGAGCCATTTCCAACTTGGATCCGGGGGAACAAACAAGTGCCCGGGAGCCT
  GGGCAAAAGGCCTGTTGAACAGAAACTGGAATTNTTTCAACTTAACGCCTTGAACATTCA
  AGGCCTGGGCCCCCGAGGGAAACCCTTTGCCCATCCCAGGAACCCCTCCGCCCCAGGGTG
  GTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012190 unedited
  ACTCTGGACCGCGGCNCGCAATCTANGATNCGGTTTTTTTTTTTTTTTTTTTTTTTTTCT
  CTTCATAAGCTTTATTCTCCCTGGGAGGGGCACACCTCACCCAGCCAAGGGCTGCTTCTG
  ACTGGACAGAGGGCGTCCAAAATGCAAACATGGTGTGCAGGCAGGAGGGCTTCCACTAGC
  CCCCCAGGTGGGAGGTGCTGTGCACCCAGGCTCAAGAGGGAGGGGGCCCCAGCCACGAGG
  GAGGGGCAGGGACTTTCTTCTCACAAAGACCTTTCTTCAGTATTCGAAGGTCACTGTCTT
  GACCCGCAGGTACTCGTTCAAAGCCGCCTCTCCTAAATCTTTGCCAAATCCAGACTGTTT
  GAATCCTCCGAAGGGAGCGGCCACGTCGGTCTTGTTGTACGTGTTGACAAACACAGTGCC
  TGCCTGGAGCTTGTCACTGACATACAGGGCCTTGTTGATGTCCCTGGTGAAGACACCAGA
  AGCCAGGCCAAATTCCGTGGCATTGGCCCGAGACAGCACGGCATCCAAGTCCCCATCAGC
  AAACCGAGAGATGATCATGACAGGCCCGAAGGACTCCTCCTTGGCTATGAACATGTGGTC
  TTCCACGTCTGTGAAAACAGTTGGCTCAAAGAAGAACCCTGGCCGAGGGACCTGATTCCC
  GCCGCAGACCAGTGTGGCCCCTTCCTTCACGCCATGCTGGCAGTACTCCATCAGCTTCAC
  AAGGTGGGCATGGTGATTCTGCGGCCCCGTGGTCGGTGTCCCTGTCCAGCGGGGTGCCCA
  CCTTCATCTTCCGCACCTTTNTACCACTCTNCGCACGAACNATCATGAATGGAGTCCTNC
  ACAAAGAGTCGCCTGCTGCATGCAATCTCTCCTTTGTTGAAGAAACAGACTCATCCCATC
  TGCACANCTTGGTGAGTCACAGTCAGCAAAATGATGAGGGTGACTTCCCGCCCAGTCAGG
  GACACTTCTNACAGTACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012190
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012190.2, NP_036322.2
• RefSeq Size: 3125 bp
• RefSeq ORF: 2709 bp
• Locus ID: 10840
• UniProt ID: O75891
• Cytogenetics: 3q21.3
• Domains: aldedh, formyl_transf, formyl_trans_C
• Protein Families: Druggable Genome
• Protein Pathways: One carbon pool by folate
• Gene Summary: The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (2) contains a distinct 5' UTR, lacks an in-frame portion of the 5' coding region, and initiates translation at an alternate in-frame downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.