ALDH1L1 (NM_012190) Human Tagged ORF Clone

CAT#: RC213720

ALDH1L1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)

Product Images

Circular map for RC213720

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ALDH1L1 (Cat# RC213720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1(Cat# TA501851). Positive lysates LY415919 (100ug) and LC415919 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (RC213720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1 (TA501869). Positive lysates LY415919 (100ug) and LC415919 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415919) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213720 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ALDH1L1 protein (Cat# TP313720). The protein was produced from HEK293T cells transfected with ALDH1L1 cDNA clone (Cat# RC213720) using MegaTran 2.0 (Cat# TT210002).

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH1L1
• Synonyms: 10-fTHF; 10-FTHFDH; FDH; FTHFD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213720 representing NM_012190
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGGAAGTTTACTGCCACCTGAGGAAGGAGGGCC
  ACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGGAAAGGCCGACCCCCTGGGTCTGGAAGCTGA
  GAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGTGCAAAAGGACAGGCTTTGCCTGATGTGGTG
  GCAAAATACCAGGCTTTGGGGGCCGAGCTCAACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAGA
  TAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCACCGAGGGGCCTC
  GGCCATCAACTGGACCCTCATTCACGGAGATAAGAAAGGGGGGTTTTCCATCTTCTGGGCGGATGATGGT
  CTGGACACCGGAGACCTGCTGCTGCAGAAGGAGTGTGAGGTGCTCCCGGACGACACCGTGAGCACGCTGT
  ACAACCGCTTCCTCTTCCCTGAAGGCATCAAAGGGATGGTGCAGGCCGTGAGGCTGATCGCTGAGGGCAA
  AGCCCCCAGACTCCCTCAGCCTGAGGAAGGAGCCACCTATGAGGGGATTCAGAAGAAGGAGACAGCCAAG
  ATCAACTGGGACCAGCCGGCAGAGGCCATTCACAACTGGATCCGCGGGAACGACAAGGTGCCGGGAGCCT
  GGACAGAGGCCTGTGAACAGAAACTGACATTTTTCAACTCAACGCTGAACACTTCAGGCCTGGTGCCCGA
  GGGAGACGCTTTGCCCATCCCAGGAGCCCATCGGCCAGGGGTGGTCACCAAAGCAGGACTCATCCTCTTT
  GGGAATGATGACAAAATGCTGCTGGTGAAGAATATTCAGCTGGAGGATGGCAAAATGATCCTGGCCTCGA
  ACTTCTTTAAGGGGGCAGCCAGCAGTGTCCTTGAGCTGACAGAGGCAGAGCTGGTTACTGCGGAGGCTGT
  GCGGAGTTTTTGGCAGCGGATCCTCCCCAAAGTCCTGGAGGTTGAAGACTCCACTGATTTCTTCAAGTCA
  GGGGCCGCGTCTGTGGACGTTGTGAGGCTGGTGGAGGAAGTGAAGGAGCTGTGTGATGGCCTGGAGTTAG
  AAAATGAAGATGTGTACATGGCATCCACCTTTGGGGACTTCATCCAGCTGTTAGTGAGGAAGCTTCGAGG
  GCACGATGAGGAGGGCGAGTGCAGCATTGACTACGTGGAAATGGCAGTGAACAAGCGCACTGTCCGCATG
  CCCCACCAGCTCTTCATTGGGGGGGAGTTCGTGGATGCCGAGGGCGCCAAGACCTCTGAGACCATCAATC
  CCACCGATGGAAGTGTCATCTGCCAGGTATCCCTGGCCCAAGTCACCGACGTCGACAAGGCAGTGGCCGC
  AGCCAAGGATGCCTTTGAGAATGGACGGTGGGGGAAGATCGGTGCGCGGGACCGGGGCCGGCTGATGTAC
  AGGTTGGCAGATCTCATGGAGCAGCACCAGGAGGAGCTGGCCACCATTGAGGCCCTGGATGCGGGTGCCG
  TCTACACGCTGGCCCTGAAGACCCACGTGGGCATGTCCATCCAGACCTTCCGCTACTTTGCTGGCTGGTG
  TGACAAGATCCAGGGCTCCACCATCCCCATCAACCAGGCCAGACCCAACCGCAACCTGACCTTGACCAGG
  AAGGAGCCTGTTGGGGTTTGTGGCATCATCATCCCCTGGAACTATCCCCTGATGATGCTGTCCTGGAAGA
  CAGCTGCCTGCCTGGCTGCCGGGAACACAGTGGTGATCAAGCCTGCTCAGGTGACCCCACTCACAGCCTT
  GAAGTTTGCAGAGCTGACATTAAAGGCCGGCATTCCCAAAGGTGTGGTTAACGTCCTCCCAGGATCTGGC
  TCCCTGGTCGGCCAGAGACTCTCAGACCATCCTGATGTGAGGAAAATCGGGTTCACAGGCTCCACAGAGG
  TGGGCAAGCACATCATGAAAAGCTGTGCCATAAGTAACGTGAAGAAGGTGTCCCTGGAACTGGGCGGGAA
  GTCACCCCTCATCATCTTTGCTGACTGTGACCTCAACAAGGCTGTGCAGATGGGGATGAGTTCTGTTTTC
  TTCAACAAAGGAGAGAATTGCATTGCAGCAGGCCGACTCTTTGTGGAGGACTCCATTCATGATGAGTTCG
  TGCGGAGAGTGGTAGAAGAGGTGCGGAAGATGAAGGTGGGCAACCCGCTGGACAGGGACACCGACCACGG
  GCCGCAGAATCACCATGCCCACCTTGTGAAGCTGATGGAGTACTGCCAGCATGGCGTGAAGGAAGGGGCC
  ACACTGGTCTGCGGCGGGAATCAGGTCCCTCGGCCAGGGTTCTTCTTTGAGCCAACTGTTTTCACAGACG
  TGGAAGACCACATGTTCATAGCCAAGGAGGAGTCCTTCGGGCCTGTCATGATCATCTCTCGGTTTGCTGA
  TGGGGACTTGGATGCCGTGCTGTCTCGGGCCAATGCCACGGAATTTGGCCTGGCTTCTGGTGTCTTCACC
  AGGGACATCAACAAGGCCCTGTATGTCAGTGACAAGCTCCAGGCAGGCACTGTGTTTGTCAACACGTACA
  ACAAGACCGACGTGGCCGCTCCCTTCGGAGGATTCAAACAGTCTGGATTTGGCAAAGATCTAGGAGAGGC
  GGCTCTGAACGAGTACCTGCGGGTCAAGACAGTGACCTTCGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213720 representing NM_012190
  Red=Cloning site Green=Tags(s)
  
  MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWRAKGQALPDVV
  AKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFSIFWADDG
  LDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAK
  INWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILF
  GNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSFWQRILPKVLEVEDSTDFFKS
  GAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGHDEEGECSIDYVEMAVNKRTVRM
  PHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKIGARDRGRLMY
  RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINQARPNRNLTLTR
  KEPVGVCGIIIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNVLPGSG
  SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCAISNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF
  FNKGENCIAAGRLFVEDSIHDEFVRRVVEEVRKMKVGNPLDRDTDHGPQNHHAHLVKLMEYCQHGVKEGA
  TLVCGGNQVPRPGFFFEPTVFTDVEDHMFIAKEESFGPVMIISRFADGDLDAVLSRANATEFGLASGVFT
  RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTFEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012190
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012190.4
• RefSeq Size: 3125 bp
• RefSeq ORF: 2709 bp
• Locus ID: 10840
• UniProt ID: O75891
• Cytogenetics: 3q21.3
• Domains: aldedh, formyl_transf, formyl_trans_C
• Protein Families: Druggable Genome
• Protein Pathways: One carbon pool by folate
• MW: 98.6 kDa
• Gene Summary: The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]