BRF1 (NM_001519) Human Untagged Clone

CAT#: SC111637

BRF1 (untagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRF1
• Synonyms: BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111637 sequence for NM_001519 edited (data generated by NextGen Sequencing)
  ATGACGGGCCGCGTGTGCCGCGGTTGCGGCGGCACGGNNNNNNAGCTGGACGCGGCGCGC
  GGGGACGCGGTGTGCACCGCCTGCGGCTCAGTGCTGGAGGACAACATCATCGTGTCCGAG
  GTGCAGTTCGTGGAGAGCAGCGGCGGCGGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTG
  GACGGTGCTGGCAAAACCCCGACTCTGGGTGGCGGCTTCCACGTGAATCTGGGGAAGGAG
  TCGAGAGCGCAGACCCTGCAGAATGGGAGGCGCCACATCCACCACCTGGGGAACCAGCTG
  CAGCTGAACCAGCACTGCCTGGACACCGCCTTCAACTTCTTCAAGATGGCCGTGAGCAGG
  CACCTGACCCGCGGCCGGAAGATGGCCCACGTGATTGCTGCCTGCCTCTACCTGGTCTGC
  CGTACGGAGGGCACGCCGCACATGCTCCTGGACCTCAGCGACCTGCTCCAGGTGAATGTG
  TACGTGCTTGGAAAGACGTTTCTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCCGGCC
  ATAGACCCGTGCCTGTATATTCCACGCTTTGCGCACCTGCTGGAATTCGGGGAGAAGAAC
  CACGAGGTGTCCATGACTGCCCTGAGGCTCCTACAGAGGATGAAGCGGGACTGGATGCAC
  ACAGGCCGGCGCCCCTCGGGCCTCTGCGGAGCAGCGCTCCTGGTTGCAGCCAGAATGCAT
  GACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTCAAAGTGTGTGAGTCCACGCTG
  CGGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACCATTGATGAGTTC
  ATGAAGATCGACCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAGGAAG
  CTGCGGATGAAGCAGCTTGAACAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAA
  ATATCCAGTTACCAGGATGCAATTGAGATTGAACTAGAAAACAGCCGGCCAAAGGCCAAG
  GGGGGCCTGGCCAGCCTGGCAAAAGATGGCTCCACCGAGGACACCGCGTCCAGCTTGTGT
  GGCGAGGAGGACACAGAGGACGAGGAGCTGGAAGCCGCGGCCAGCCACCTGAACAAAGAC
  TTATACCGGGAGCTCCTTGGTGGTGCCCCCGGCAGCTCGGAAGCAGCAGGAAGCCCCGAG
  TGGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCCCTCCCCACTGCAGCCAGC
  CTGGGCATCTCAGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGACCCCAAAGAT
  GCTTCAGGAGACGGTGAGCTGGACCTCAGTGGCATTGATGACCTGGAGATTGACAGGTAC
  ATCCTGAATGAGTCGGAAGCCCGCGTGAAGGCCGAGCTGTGGATGAGGGAGAACGCCGAG
  TACCTGCGGGAACAGAGGGAAAAAGAAGCAAGAATAGCGAAAGAGAAGGAGCTCGGCATC
  TACAAGGAACACAAGCCCAAGAAGTCTTGCAAGCGACGGGAGCCAATTCAGGCCAGTACC
  GCCAGGGAGGCCATCGAGAAGATGCTGGAGCAGAAGAAGATCTCCAGCAAGATCAATTAT
  AGCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGTCCGCACAGGGAGGATGCACAG
  CCCGAGCATAGCGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCGGCCAGCAGAAGT
  GGGGCTGACCCTGTGACCAGTGTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAGCCA
  GCAAAGAAGGTGGCCACGGGAGAGGCTTTGCTCCCAAGCTCTCCCACCCTCGGAGCTGAG
  CCTGCCAGGCCCCAGGCGGTGCTGGTGGAGAGCGGGCCCGTGTCATACCACGCCGACGAG
  GAGGCTGACGAGGAGGAGCCTGACGAGGAGGACGGGGAGCCCTGCGTCAGTGCCCTGCAG
  ATGATGGGCAGCAACGACTATGGCTGTGATGGCGATGAGGACGACGGCTACTGA
  
  Clone variation with respect to NM_001519.3
  38 a=>n;39 c=>n;40 a=>n;41 t=>n;42 c=>n;43 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001519 unedited
  TCGGAANNGNATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGGAGAGTCTTGG
  GAGGCTCCGGGCGCTGCCCGCGCGGTCGCCGGCATGACGGGCCGCGTGTGCCGCGGTTGC
  GGCGGCACGGACATCGAGCTGGACGCGGCGCGCGGGGACGCGGTGTGCACCGCCTGCGGC
  TCAGTGCTGGAGGACAACATCATCGTGTCCGAGGTGCAGTTCGTGGAGAGCAGCGGCGGC
  GGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTGGACGGTGCTGGCAAAACCCCGACTCTG
  GGTGGCGGCTTCCACGTGAATCTGGGGAAGGAGTCGAGAGCGCAGACCCTGCAGAATGGG
  AGGCGCCACATCCACCACCTGGGGAACCAGCTGCAGCTGAACCAGCACTGCCTGGACACC
  GCCTTCAACTTCTTCAAGATGGCCGTGAGCAGGCACCTGACCCGCGGCCGGAAGATGGCC
  CACGTGATTGCTGCCTGCCTCTACCTGGTCTGCCGTACGGAGGGCACGCCGCACATGCTC
  CTGGACCTCAGCGACCTGCTCCAGGTGAATGTGTACGTGCTTGGAAAGACGTTTCTTCTC
  TTGGCAAGAGAGCTCTGCATCAATGCGCCGGCCATAGACCCGTGCCTGTATATTCCACGC
  TTTGCGCACCTGCTGGAATTCGGGGAGAAAGAACCACGAGTGTCCATGACTGCCCTGAGG
  CTNCTACAGNNAGATGAAAGCGGACTGGATGCACACAGGCCGGGCGCCCCTCGGCCTCTG
  CGGAGCAGCGCTCCTGGNTGCAGCCAGAATGCATGACTTNCAGAGGACTGTGAAAGGAGT
  CATCAGTGTGGTCAAAAGTGTGTGAGTCCACGCTGCNGNAGAAGCTCACGGATTTNGAGA
  ACCCCCACAGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001519 unedited
  GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTGGTAAAGAACAACAGGAACTTCCTGGTTTACATAAAACTAT
  TCTTCCCCCAAACCCTTGAAAAACCCTGGCAGGAACCAAGCTGGCTACTGAGCTCTGGGC
  GGGGGTAGGGGGGTCTGGCCTGCTGCGGGCCAAGTTGGGGCAAGACATACAGCCCATTTC
  ATGGGGAGGATGAGGCCCCTGGGGGGCAATGAGGGACGGGTCTGGCTCAAGGGCACCCCC
  TCCTAAGGACACAGGGGGAAGCCCCCTCGGCCACATTCGGGGCAGCCATGCCAGAGCTGA
  GACCTCCTACGAGGGGGACCGGGGCCCTCCCCCAAAAGGCCTGGGGAGGCTCTCGGGCCT
  CCGGCTGGCCTTCCTTCTCGATGGGGGGCCCGAACCCGCTTTGACCGGGAGCGCCCCTGG
  CCTCTGGGTTTTGTCACGGCAACTTCCAAAGCCCGGACCTATGCCGGCTATGCCCGCACT
  GCCCAACGAACTTTGCTCAGGACGGGGGGGCGCCGGCTCAAATTGTCGGCCCTAAGTCAC
  CTGGCGGCAGGCCCAAGGTTTCTGGGGCCACAAACCAAGGGGGCCTGGAGCAAGGGTCCG
  ACCCCCCTGCTGGTCCAGCGGCGCCAGCACGGGCACGGGCGGCTTGTCGACGCCCGCCTC
  TCCCCCTTCCCACCCCCTCCGCGTCCCCCCCCCCCCCCCCATTCCGCTCCCCTTTTTTTT
  TTCCTTGTTTTTCCCCCCCCCCCCCCCCCTTACGCCCCTTTTCCTTTTCCCTTCCCCCCC
  TTCCCTCCTCCCCCCTCCCCCCCTTCTCCCCCCCTCCCTCCCCCCCTTCCCACTTCTCAC
  CCACTCCCCTCCCCTTTCCTCCCCCCTTTATTCCCCTCCCCCCTCCACCCCCCCCTGCCC
  CTCCCCACCCCCTTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001519
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001519.2, NP_001510.2
• RefSeq Size: 3660 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2972
• UniProt ID: Q92994
• Cytogenetics: 14q32.33
• Domains: transcript_fac2, CYCLIN
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).