AKAP12 (NM_005100) Human Untagged Clone

CAT#: SC110078

AKAP12 (untagged)-Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKAP12
• Synonyms: AKAP250; SSeCKS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005100 edited
  TTCGGCACGAGGGTTTGCCGCGAGCGCGTCTCCTTCATTCGCAGGCTGGGCGCGTTCGCA
  GTCGGCTGGCGGCGAAGGAAGGCGCTCTCGGGACCTCGCGGGCGCGCGTCTTTTGGCTCT
  TGCCCCTGTCCCTGCGGCTTGGGGAAGGCGTAACCCGGCGGCTAGGCGCGGGAGAAGTGC
  GGAGGAGCCATGGGCGCCGGGAGCTCCACCGAGCAGCGCAGCCCGGAGCAGCCGCCCGAG
  GGGAGCTCCACGCCGGCTGAGCCCGAGCCCAGCGGCGGCGGCCCCTCGGCCGAGGCGGCG
  CCAGACACCACCGCGGACCCCGCCATCGCTGCCTCGGACCCCGCCACCAAGCTCCTACAG
  AAGAATGGTCAGCTGTCCACCATCAATGGCGTAGCTGAGCAAGATGAGCTCAGCCTCCAG
  GAGGGTGACCTAAATGGCCAGAAAGGAGCCCTGAACGGTCAAGGAGCCCTAAACAGCCAG
  GAGGAAGAAGAAGTCATTGTCACAGAGGTTGGACAGAGAGACTCTGAAGATGTGAGCAAA
  AGAGACTCCGATAAAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGAT
  GGGCAGGAGGAGACACCCGAAATAATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAA
  GAGCTAACACAACCCACTGAGTCCCAGGCTAATGATATTGGATTTAAGAAGGTGTTTAAG
  TTTGTTGGCTTTAAATTCACTGTGAAAAAGGATAAGACAGAGAAGCCTGACACTGTCCAG
  CTACTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCAGGGGCTGGCGACCACAAGGAC
  CCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAACAATCTACAGAG
  AAACCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGAA
  TCTGGCCAAGCAGTGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCT
  AGCAAGTCTGCAGAATCTCCGACTAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAA
  AAATTCTTCACTCAAGGTTGGGCCGGCTGGCGCAAAAAGACCAGTTTCAGGAAGCCGAAG
  GAGGATGAAGTGGAAGCTTCAGAGAAGAAAAAGGAACAAGAGCCAGAAAAAGTAGACACA
  GAAGAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTGACCGCCTCCGAGCAAGCCCAC
  CCACAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAATATGAGAAAGTT
  GAGCTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGCT
  CCGTTGGCGACAGAAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCC
  GAAGTCCACGTCAGCACCGTGGAGGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAA
  ACAGCAGGGTCTGTGCCAGCTGAAGAATTGGTTGAAATGGATGCAGAACCTCAGGAAGCT
  GAACCTGCCAAGGAGCTGGTGAAGCTCAAAGAAACGTGTGTTTCCGGAGAGGACCCTACA
  CAGGGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCCAAACCCCCCGAAGGCGTTGTG
  AGTGAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAAGTCCACTAAAG
  AAGCTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAGA
  GGAGGAGGAGACGAGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGC
  CAGGAGGAGCAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACG
  TGTCTGGAAAAGGGCTTAGCCGAGGTGCAGCAGGATGGGGAAGCTGAAGAAGGAGCTACT
  TCCGATGGAGAGAAAAAAAGAGAAGGTGTCACTCCCTGGGCATCATTCAAAAAGATGGTG
  ACGCCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGATAAAGAAGATGAGCTGGACAAG
  GTCAAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGCAAGAAGAAATG
  AAAGGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGTA
  TCTTGGGAAGCTTTAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCT
  TCTGATGAGGAAGGGGGACCAAAAGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCC
  GGAAAAGACAAAGAGACGGGGACAGACGGGATCCTTGCTGGTTCCCAAGAACATGATCCA
  GGGCAGGGAAGTTCCTCCCCGGAGCAAGCTGGAAGCCCTACCGAAGGGGAGGGCGTTTCC
  ACCTGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAAAAATCAAAGTCCAAGCTGGAA
  GAGAAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTCCAGACACTGAA
  CCCGGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAAA
  AGGCCAGATGGGAAACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAAC
  GAAGATGACTCTGATGTCCCGGCCGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGG
  GAGAAAATGGAGGCACAGCAAGCCCAAAAAAGCGCAGAGCAGCCCGAGCAGAAGGCAGCC
  ACTGAGGTGTCCAAGGAGCTCAGCGAGAGTCAGGTTCATATGATGGCAGCAGCTGTCGCT
  GACGGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCTCCTTCTTGGATATCTGCTTCA
  GTGACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTGAGGAGGTATTG
  GAAAGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAAC
  AGAGAGGCCCGGGGCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACA
  GCTGCAGAAACTGCAGGGCCATTGGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAA
  GAGACCACAGAAATGGTGTCAGCAGTCTCCCAGTTAACCGACTCCCCAGACACCACAGAG
  GAGGCCACTCCGGTGCAGGAGGTGGAAGGTGGCGTACCTGACATAGAAGAGCAAGAGAGG
  CGGACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTGAAAGAGGAATCCCAGCTGCCT
  GGCACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGGCAGAAAGACCA
  GAAGAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGAT
  GCTCAGGAGGCAAAAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCA
  GAAAGCTTTGAAAAAGCTCCTCAAGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACC
  ACTTGTCAAGCCGAAACCTTAGCTGGGGTAAAATCACAGGAGATGGTGATGGAACAGGCT
  ATCCCCCCTGACTCGGTGGAAACCCCTACAGACAGTGAGACTGATGGAAGCACCCCCGTA
  GCCGACTTTGACGCACCAGGCACAACCCAGAAAGACGAGATTGTGGAAATCCATGAGGAG
  AATGAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAGTTCCTGCACAG
  AAAGAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATCA
  AAGATGGAAGACACTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATT
  CTGTCAAAGACTGAGGGGACTCAAGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGAC
  GTACCATTTTTCGAAGGACTTGAGGGGTCTATAGACACAGGCATAACAGTCAGTCGGGAA
  AAGGTCACTGAAGTTGCCCTTAAAGGTGAAGGGACAGAAGAAGCTGAATGTAAAAAGGAT
  GATGCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCATCCCCCGTGGAGAGAGAGATG
  GTAGTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGAATGAAGAGAAG
  CTTGAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGACA
  GTGAATGTGCCCATCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCT
  CCCTGCCTAGGTCAAGAGGAGGCAGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCA
  TCATTCACTCTAACAGCGGCTGCAGAGGAGGAAAAGGTCTTAGGAGAAACTGCCAACATT
  TTAGAAACAGGTGAAACGTTGGAGCCTGCAGGTGCACATTTAGTTCTGGAAGAGAAATCC
  TCTGAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAAGATGCTGTGCCCACAGGGCCC
  GACTGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGAAAGGCTTAAGT
  TCGGACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGAG
  CAGGTTGCTTGCCAGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAAT
  GGGATTTTGGAACTTGAGACCAAAAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCC
  GTTGACCAGTTTGTACGTACAGAAGAAACAGCCACCGAAATGTTGACGTCTGAGTTACAG
  ACACAAGCTCACGTGATAAAAGCTGACAGCCAGGACGCTGGACAGGAAACGGAGAAAGAA
  GGAGAGGAACCTCAGGCCTCTGCACAGGATGAAACACCAATTACTTCAGCCAAAGAGGAG
  TCAGAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACATGAGTGAAGCC
  TCAGAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGAA
  GAGGTCGTCCTCCCATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAA
  GATGATGGTCATGCCTTGTTAGCAGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAA
  GATGAAAAAGGTGATGATGTTGATGACCCTGAAAACCAGAACTCAGCCCTGGCTGATACT
  GATGCCTCAGGAGGCTTAACCAAAGAGTCCCCAGATACAAATGGACCAAAACAAAAAGAG
  AAGGAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAAGTGCACAGTGAATCAGATAAA
  GCGATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAATCTGCAAAGTCA
  GAACTTACAGAATCTTAAAACATCATGCAGTTAAACTCATTGTCTGTTTGGAAGACCAGA
  ATGTGAAGACAAGTAGTAGAAGAAAATGAATGCTGCTGCTGAGACTGAAGACCAGTATTT
  CAGAACTTTGAGAATTGGAGAGCAGGCACATCAACTGATCTCATTTCTAGAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005100 unedited
  TGAATTAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGTTTGCCGCGAGCG
  CGTCTCCTTCATTCGCAGGCTGGGCGCGTTCGCAGTCGGCTGGCGGCGAAGGAAGGCGCT
  CTCGGGACCTCGCGGGCGCGCGTCTTTTGGCTCTTGCCCCTGTCCCTGCGGCTTGGGGAA
  GGCGTAACCCGGCGGCTAGGCGCGGGAGAAGTGCGGAGGAGCCATGGGCGCCGGGAGCTC
  CACCGAGCAGCGCAGCCCGGAGCAGCCGCCCGAGGGGAGCTCCACGCCGGCTGAGCCCGA
  GCCCAGCGGCGGCGGCCCCTCGGCCGAGGCGGCGCCAGACACCACCGCGGACCCCGCCAT
  CGCTGCCTCGGACCCCGCCACCAAGCTCCTACAGAAGAATGGTCAGCTGTCCACCATCAA
  TGGCGTAGCTGAGCAAGATGAGCTCAGCCTCCAGGAGGGTGACCTAAATGGCCAGAAAGG
  AGCCCTGAACGGTCAAGGAGCCCTAAACAGCCAGGAGGAAGAAGAAGTCATTGTCACAGA
  GGTTGGACAGAGAGACTCTGAAGATGTGAGCAAAAGAGACTCCGATAAAGAGATGGCTAC
  TAAGTCAGCGGTTGTTCACGACATCACAGATGATGGGCAGGAGGAGACACCCGAAATAAT
  CGAACAGATTCCTTCTTCAGANAGCAATTTAGAAGAGCTAACACAACCCACTGAGTCCCA
  GGCTAATGATATTGGATTTAAGAAGGTGGTTAAGTTTGTTGGCTTTAAAATCACTGTNGA
  AAAGGATNAGACAGAGAAGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGG
  GGAGGGAGCAGCAGGNGCTGGCGACCACAAGGACCCAGCCTTGNNGCTGNAGAAGCAGCA
  TCCACAGAAGCGAACCCAACATCTACGAGAAACCCGAGAGACCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005100 unedited
  TGTAACGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTAAGTGAAGCAAA
  TATTTTATTTAAATCAGTCGTCAAATCACAACTTATCCAAATGAATGTGTTGCAACATTG
  TTCTTAAAAGAATGGCACAAATATGGCACAAACACAATCCAGTATCTATCAAAATACCAT
  TTGTAAAGAACCTGACTTATTTCATGCCATGTGTGAATGCAGTCCAGCATATAAGAGTAG
  AATCAAAAACTTACAACAATTCTTTTCTTCATAACATATAAAACATATTCACTACTTTGC
  CTCAGGATTAAGCACACTTATCGGTCCAAACAACCATAACAAGTAGGAAAAAGGATGTAA
  AGTTAATTTGCTACCAGAATATAACTTCTTTCTAACCTCCACTGGGTTAGAAAGAAAAAT
  TTCCCAATGACAATAGAACATTCTGTATGTTTCAAAATGCCTTTTTAGTTTTAGCACACT
  CCGTGTAATAGGAACGTGACCAATTTCAGAGCACCACTAAATATGGACCTCAATCATTAG
  CGAATTTTTAAAGAATTGCACCTTGATCAGGAATGTTACATCTAGGAGGCTCTGTTTTTC
  CGGGCTATATAACTGAAGCTTAAAGCCTCCCAAGCCTGCACTATGGCACATGCCCCATCA
  TATACTGGGGGATACAAAAACCTGGAGTATCCTGCCCATTCCCTTATACTTTAGAATAAA
  TTTCCACTTGATACCGGAGGACTACTTAACCACTTCATATAAATTTGGAAAGAATGGATA
  CTCAATTTCCACAACCAAAACTAGGTTTTGCCAACTTCAGTTCCAGGTTAAGAATTTAAG
  GATCTCTAATCACAATCTATTAGGCGTTCAAGCGAAATCGGCCGTTGGTCCTTGCAACAG
  AAAAGGCAAAGGCCCTAACCTCCGATAAACCCTAGGAATGCCAGAGGCCTTCTAAAAGTA
  ATACATGAGGGCCCGGCTCCACATTCCAAANTTTGAAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005100
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005100.2, NP_005091.2
• RefSeq Size: 6609 bp
• RefSeq ORF: 5349 bp
• Locus ID: 9590
• UniProt ID: Q02952
• Cytogenetics: 6q25.1
• Domains: PkinA_anch
• Protein Families: Druggable Genome
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.