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AKAP12 (NM_005100) Human Tagged ORF Clone
CAT#: RG221620
- TrueORF®
AKAP12 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 1
ORF Plasmid: DDK
"NM_005100" in other vectors (2)
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Other products for "AKAP12"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | AKAP12 |
| Synonyms | AKAP250; SSeCKS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221620 representing NM_005100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCGGGAGCTCCACCGAGCAGCGCAGCCCGGAGCAGCCGCCCGAGGGGAGCTCCACGCCGGCTG AGCCCGAGCCCAGCGGCGGCGGCCCCTCGGCCGAGGCGGCGCCAGACACCACCGCGGACCCCGCCATCGC TGCCTCGGACCCCGCCACCAAGCTCCTACAGAAGAATGGTCAGCTGTCCACCATCAATGGCGTAGCTGAG CAAGATGAGCTCAGCCTCCAGGAGGGTGACCTAAATGGCCAGAAAGGAGCCCTGAACGGTCAAGGAGCCC TAAACAGCCAGGAGGAAGAAGAAGTCATTGTCACAGAGGTTGGACAGAGAGACTCTGAAGATGTGAGCAA AAGAGACTCCGATAAAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGATGGGCAGGAG GAGACACCCGAAATAATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAAGAGCTAACACAACCCACTG AGTCCCAGGCTAATGATATTGGATTTAAGAAGGTGTTTAAGTTTGTTGGCTTTAAATTCACTGTGAAAAA GGATAAGACAGAGAAGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCA GGGGCTGGCGACCACAAGGACCCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAAC AATCTACAGAGAAACCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGA ATCTGGCCAAGCAGTGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCTAGCAAGTCT GCAGAATCTCCGACTAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAAAAATTCTTCACTCAAGGTT GGGCCGGCTGGCGCAAAAAGACCAGTTTCAGGAAGCCGAAGGAGGATGAAGTGGAAGCTTCAGAGAAGAA AAAGGAACAAGAGCCAGAAAAAGTAGACACAGAAGAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTG ACCGCCTCCGAGCAAGCCCACCCACAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAAT ATGAGAAAGTTGAGCTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGC TCCGTTGGCGACAGAAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCCGAAGTCCAC GTCAGCACCGTGGAGGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAAACAGCAGGGTCTGTGCCAG CTGAAGAATTGGTTGAAATGGATGCAGAACCTCAGGAAGCTGAACCTGCCAAGGAGCTGGTGAAGCTCAA AGAAACGTGTGTTTCCGGAGAGGACCCTACACAGGGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCC AAACCCCCCGAAGGCGTTGTGAGTGAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAA GTCCACTAAAGAAGCTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAG AGGAGGAGGAGACGAGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGCCAGGAGGAG CAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACGTGTCTGGAAAAGGGCTTAG CCGAGGTGCAGCAGGATGGGGAAGCTGAAGAAGGAGCTACTTCCGATGGAGAGAAAAAAAGAGAAGGTGT CACTCCCTGGGCATCATTCAAAAAGATGGTGACGCCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGAT AAAGAAGATGAGCTGGACAAGGTCAAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGC AAGAAGAAATGAAAGGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGT ATCTTGGGAAGCTTTAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCTTCTGATGAG GAAGGGGGACCAAAAGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCCGGAAAAGACAAAGAGACGG GGACAGACGGGATCCTTGCTGGTTCCCAAGAACATGATCCAGGGCAGGGAAGTTCCTCCCCGGAGCAAGC TGGAAGCCCTACCGAAGGGGAGGGCGTTTCCACCTGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAA AAATCAAAGTCCAAGCTGGAAGAGAAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTC CAGACACTGAACCCGGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAA AAGGCCAGATGGGAAACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAACGAAGATGAC TCTGATGTCCCGGCCGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGGGAGAAAATGGAGGCACAGC AAGCCCAAAAAAGCGCAGAGCAGCCCGAGCAGAAGGCAGCCACTGAGGTGTCCAAGGAGCTCAGCGAGAG TCAGGTTCATATGATGGCAGCAGCTGTCGCTGACGGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCT CCTTCTTGGATATCTGCTTCAGTGACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTG AGGAGGTATTGGAAAGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAA CAGAGAGGCCCGGGGCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACAGCTGCAGAA ACTGCAGGGCCATTGGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAAGAGACCACAGAAATGGTGT CAGCAGTCTCCCAGTTAACCGACTCCCCAGACACCACAGAGGAGGCCACTCCGGTGCAGGAGGTGGAAGG TGGCGTACCTGACATAGAAGAGCAAGAGAGGCGGACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTG AAAGAGGAATCCCAGCTGCCTGGCACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGG CAGAAAGACCAGAAGAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGA TGCTCAGGAGGCAAAAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCAGAAAGCTTT GAAAAAGCTCCTCAAGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACCACTTGTCAAGCCGAAACCT TAGCTGGGGTAAAATCACAGGAGATGGTGATGGAACAGGCTATCCCCCCTGACTCGGTGGAAACCCCTAC AGACAGTGAGACTGATGGAAGCACCCCCGTAGCCGACTTTGACGCACCAGGCACAACCCAGAAAGACGAG ATTGTGGAAATCCATGAGGAGAATGAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAG TTCCTGCACAGAAAGAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATC AAAGATGGAAGACACTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATTCTGTCAAAG ACTGAGGGGACTCAAGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGACGTACCATTTTTCGAAGGAC TTGAGGGGTCTATAGACACAGGCATAACAGTCAGTCGGGAAAAGGTCACTGAAGTTGCCCTTAAAGGTGA AGGGACAGAAGAAGCTGAATGTAAAAAGGATGATGCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCA TCCCCCGTGGAGAGAGAGATGGTAGTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGA ATGAAGAGAAGCTTGAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGAC AGTGAATGTGCCCATCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCTCCCTGCCTA GGTCAAGAGGAGGCAGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCATCATTCACTCTAACAGCGG CTGCAGAGGAGGAAAAGGTCTTAGGAGAAACTGCCAACATTTTAGAAACAGGTGAAACGTTGGAGCCTGC AGGTGCACATTTAGTTCTGGAAGAGAAATCCTCTGAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAA GATGCTGTGCCCACAGGGCCCGACTGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGA AAGGCTTAAGTTCGGACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGA GCAGGTTGCTTGCCAGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAATGGGATTTTG GAACTTGAGACCAAAAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCCGTTGACCAGTTTGTACGTA CAGAAGAAACAGCCACCGAAATGTTGACGTCTGAGTTACAGACACAAGCTCACGTGATAAAAGCTGACAG CCAGGACGCTGGACAGGAAACGGAGAAAGAAGGAGAGGAACCTCAGGCCTCTGCACAGGATGAAACACCA ATTACTTCAGCCAAAGAGGAGTCAGAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACA TGAGTGAAGCCTCAGAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGA AGAGGTCGTCCTCCCATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAAGATGATGGT CATGCCTTGTTAGCAGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAAGATGAAAAAGGTGATGATG TTGATGACCCTGAAAACCAGAACTCAGCCCTGGCTGATACTGATGCCTCAGGAGGCTTAACCAAAGAGTC CCCAGATACAAATGGACCAAAACAAAAAGAGAAGGAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAA GTGCACAGTGAATCAGATAAAGCGATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAAT CTGCAAAGTCAGAACTTACAGAATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221620 representing NM_005100
Red=Cloning site Green=Tags(s) MGAGSSTEQRSPEQPPEGSSTPAEPEPSGGGPSAEAAPDTTADPAIAASDPATKLLQKNGQLSTINGVAE QDELSLQEGDLNGQKGALNGQGALNSQEEEEVIVTEVGQRDSEDVSKRDSDKEMATKSAVVHDITDDGQE ETPEIIEQIPSSESNLEELTQPTESQANDIGFKKVFKFVGFKFTVKKDKTEKPDTVQLLTVKKDEGEGAA GAGDHKDPSLGAGEAASKESEPKQSTEKPEETLKREQSHAEISPPAESGQAVEECKEEGEEKQEKEPSKS AESPTSPVTSETGSTFKKFFTQGWAGWRKKTSFRKPKEDEVEASEKKKEQEPEKVDTEEDGKAEVASEKL TASEQAHPQEPAESAHEPRLSAEYEKVELPSEEQVSGSQGPSEEKPAPLATEVFDEKIEVHQEEVVAEVH VSTVEERTEEQKTEVEETAGSVPAEELVEMDAEPQEAEPAKELVKLKETCVSGEDPTQGADLSPDEKVLS KPPEGVVSEVEMLSSQERMKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHTQVPADSPDSQEE QKGESSASSPEEPEEITCLEKGLAEVQQDGEAEEGATSDGEKKREGVTPWASFKKMVTPKKRVRRPSESD KEDELDKVKSATLSSTESTASEMQEEMKGSVEEPKPEEPKRKVDTSVSWEALICVGSSKKRARRGSSSDE EGGPKAMGGDHQKADEAGKDKETGTDGILAGSQEHDPGQGSSSPEQAGSPTEGEGVSTWESFKRLVTPRK KSKSKLEEKSEDSIAGSGVEHSTPDTEPGKEESWVSIKKFIPGRRKKRPDGKQEQAPVEDAGPTGANEDD SDVPAVVPLSEYDAVEREKMEAQQAQKSAEQPEQKAATEVSKELSESQVHMMAAAVADGTRAATIIEERS PSWISASVTEPLEQVEAEAALLTEEVLEREVIAEEEPPTVTEPLPENREARGDTVVSEAELTPEAVTAAE TAGPLGAEEGTEASAAEETTEMVSAVSQLTDSPDTTEEATPVQEVEGGVPDIEEQERRTQEVLQAVAEKV KEESQLPGTGGPEDVLQPVQRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESF EKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDSETDGSTPVADFDAPGTTQKDE IVEIHEENEVASGTQSGGTEAEAVPAQKERPPAPSSFVFQEETKEQSKMEDTLEHTDKEVSVETVSILSK TEGTQEADQYADEKTKDVPFFEGLEGSIDTGITVSREKVTEVALKGEGTEEAECKKDDALELQSHAKSPP SPVEREMVVQVEREKTEAEPTHVNEEKLEHETAVTVSEEVSKQLLQTVNVPIIDGAKEVSSLEGSPPPCL GQEEAVCTKIQVQSSEASFTLTAAAEEEKVLGETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGE DAVPTGPDCQAKSTPVIVSATTKKGLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGIL ELETKSSKLVQNIIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETP ITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDG HALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGK VHSESDKAITPQAQEELQKQERESAKSELTES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005100 |
| ORF Size | 5346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005100.2, NP_005091.2 |
| RefSeq Size | 6609 bp |
| RefSeq ORF | 5349 bp |
| Locus ID | 9590 |
| UniProt ID | Q02952 |
| Cytogenetics | 6q25.1 |
| Domains | PkinA_anch |
| Protein Families | Druggable Genome |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
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