WDR61 (NM_025234) Human Untagged Clone

CAT#: SC110036

WDR61 (untagged)-Human WD repeat domain 61 (WDR61)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR61
• Synonyms: REC14; SKI8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110036 sequence for NM_025234 edited (data generated by NextGen Sequencing)
  ATGACCAACCAGTACGGTATTCTCTTCAAACAAGAGCAAGCCCATGATGATGCCATTTGG
  TCAGTTGCTTGGGGGACAAACAAGAAGGAAAACTCTGAGACAGTGGTCACAGGCTCCCTA
  GATGACCTGGTGAAGGTCTGGAAATGGCGTGATGAGAGGCTGGACCTACAGTGGAGTCTG
  GAGGGACATCAGCTGGGAGTGGTGTCTGTGGACATCAGCCACACCCTGCCCATTGCTGCA
  TCCAGCTCTCTTGATGCTCATATTCGTCTTTGGGACTTGGAAAATGGCAAACAGATAAAG
  TCCATAGATGCAGGACCTGTGGATGCCTGGACTTTGGCCTTTTCTCCTGATTCCCAGTAT
  CTGGCCACAGGAACTCATGTCGGGAAAGTGAACATTTTTGGTGTGGAAAGTGGGAAAAAG
  GAATATTCTTTGGACACGAGAGGAAAATTCATTCTTAGTATTGCATATAGTCCTGATGGG
  AAATACCTAGCCAGTGGAGCCATAGATGGAATCATCAATATTTTTGATATTGCAACTGGA
  AAACTTCTGCATACCCTGGAAGGCCATGCCATGCCCATTCGCTCCTTGACCTTTTCCCCG
  GACTCCCAGCTCCTTGTCACTGCTTCAGATGATGGCTACATCAAGATCTATGATGTACAA
  CATGCCAATTTGGCTGGCACGCTGAGCGGCCATGCCTCCTGGGTGCTGAACGTTGCATTC
  TGTCCTGATGACACTCACTTTGTTTCCAGTTCGTCTGACAAAAGTGTAAAAGTTTGGGAT
  GTTGGAACGAGGACTTGTGTTCACACCTTCTTTGATCACCAGGATCAGGTCTGGGGAGTA
  AAATACAATGGAAATGGTTCAAAAATTGTGTCTGTTGGAGATGACCAGGAAATTCACATC
  TATGATTGTCCAATTTAA
  
  Clone variation with respect to NM_025234.1
  78 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025234 unedited
  GCCATTTTTGTNAATACGAACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGATCTTT
  GCGCGTCGCAGTCGCGCGGAGCCCGGCTTCCGACGTGCAGCCTGGCAGTGCAGTGAGCTG
  TCTGGCCTTTTGTCCTTGATCCTTGGTTAAGGAAATGACCAACCAGTACGGTATTCTCTT
  CAAACAAGAGCAAGCCCATGATGATGCCATTTGGTCAGTTGCTTGGGGGACAAACAAGAA
  GGAAAACTCTGAGACAGTGGTCACAGGCTCCCTAGATGACCTGGTGAAGGTCTGGAAATG
  GCGTGATGAGAGGCTGGACCTACAGTGGAGTCTGGAGGGACATCAGCTGGGAGTGGTGTC
  TGTGGACATCAGCCACACCCTGCCCATTGCTGCATCCAGCTCTCTTGATGCTCATATTCG
  TCTTTGGGACTTGGAAAATGGCAAACAGATAAAGTCCATAGATGCAGGACCTGTGGATGC
  CTGGACTTTGGCCTTTTCTCCTGATTCCCAGTATCTGGCCACAGGAACTCATGTCGGGAA
  AGTGAACATTTTTGGTGTGGAAAGTGGGAAAAAGGAATATTCTTTGGACACGAGAGGAAA
  ATTCATTCTTAGTATTGCATATAGTCCTGATGGGAAATACCTAGCCAGTGGAGCCATAGA
  TGGAATTATCAATCATTTTGATTTTGCAACTGGGAAAATTCTTGCTACCCCTGAAGGCCA
  TGCCATGCCCATTTCCTCCTTGACCTTTTCCCCGACTTCCAATTCCTTGCCACTGTTTCA
  AAGAGGGCTCCATAAGCACTATGATGACAACATGCCATTTTGCCTGCACGCTGAGGGCCC
  ATGCTCCTGGGGGCTACGTTGCTTTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025234 unedited
  NAGCTTGNACCCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCATTAAAAA
  AGCTTTATGTTAAAGTATGAACAGCATTTCCAATAAAACATGAAAAGATTCATATTGTAT
  CTACAAAATTTAAGTCTTTGCTACAATAAATGCTATATTTCTTTTAAACAAGCCTAAGAA
  GGTAAGGAATGTCATGATCAATCCGTACATTCTCTTTGCAGCATAAGCCTGGAGACTTTG
  ATGTTTAAATTGGACAATCATAGATGTGAATTTCCTGGTCATCTCCAACAGACACAATTT
  TTGAACCATTTCCATTGTATTTTACTCCCCAGACCTGATCCTGGTGATCAAAGAAGGTGT
  GAACACAAGTCCTCGTTCCAACATCCCAAACTTTTACACTTTTGTCAGACGAACTGGAAA
  CAAAGTGAGTGTCATCAGGACAGAATGCAACGTTCAGCACCCAGGAGGCATGGCCGCTCA
  GCGTGCCAGCCAAATTGGCATGTTGTACATCATAGATCTTGATGTAGCCATCATCTGAAG
  CAGTGACAAGGAGCTGGGAGTCCGGGGAAAAGGTCAAGGAGCGAATGGGCATGGCATGGC
  CTTCCAGGGTATGCAGAAGTTTTCCAGTTGCAATATCAAAAATATTGATGATTCCATCTA
  TGGCTCCACTGGCTAGGTATTTCCCATCANGACTATATGCAATACTAAGAATGAATTTTC
  CTCTCGTGTTCAAAGAATATTNCTTTTTTCCCACTTTCCACACCAAAATGTTCACTTTTC
  CCGCCTGAGTTCCCGCGGCCAGATACTGGGGATCAAGAGAATAAGCCAAAGTCCAGCCTC
  CACAAGTCCTGCATTTATGAACTTTATGTGTTTGCCTTTTCCCAGGTCCCAAGACCATTA
  TGACCCTCAAGAAAGCTGGATGCACAATTGGCCACGGTTGTGCCTTAAGTCCACAAACAC
  CCTCCAACTCTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025234
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025234.1, NP_079510.1
• RefSeq Size: 1205 bp
• RefSeq ORF: 918 bp
• Locus ID: 80349
• UniProt ID: Q9GZS3
• Cytogenetics: 15q25.1
• Domains: WD40
• Protein Pathways: RNA degradation
• Gene Summary: WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).