Doublecortin (DCX) (NM_178152) Human Untagged Clone

CAT#: SC109146

DCX (untagged)-Human doublecortin (DCX), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Doublecortin
• Synonyms: DBCN; DC; LISX; SCLH; XLIS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178152, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACTTGATTTTGGACACTTTGACGAAAGAGATAAGACATCCAGGAACATGCGAGGCTCCCGGATGA
  ATGGGTTGCCTAGCCCCACTCACAGCGCCCACTGTAGCTTCTACCGAACCAGAACCTTGCAGGCACTGAG
  TAATGAGAAGAAAGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGCTACTTCAAGGGGATTGTGTAC
  GCTGTGTCCTCTGACCGTTTTCGCAGCTTTGACGCCTTGCTGGCTGACCTGACGCGATCTCTGTCTGACA
  ACATCAACCTGCCTCAGGGAGTGCGTTACATTTACACCATTGATGGATCCAGGAAGATCGGAAGCATGGA
  TGAACTGGAGGAAGGGGAAAGCTATGTCTGTTCCTCAGACAACTTCTTTAAAAAGGTGGAGTACACCAAG
  AATGTCAATCCCAACTGGTCTGTCAACGTAAAAACATCTGCCAATATGAAAGCCCCCCAGTCCTTGGCTA
  GCAGCAACAGTGCACAGGCCAGGGAGAACAAGGACTTTGTGCGCCCCAAGCTGGTTACCATCATCCGCAG
  TGGGGTGAAGCCTCGGAAGGCTGTGCGTGTGCTTCTGAACAAGAAGACAGCCCACTCTTTTGAGCAAGTC
  CTCACTGATATCACAGAAGCCATCAAACTGGAGACCGGGGTTGTCAAAAAACTCTACACTCTGGATGGAA
  AACAGGTAACTTGTCTCCATGATTTCTTTGGTGATGATGATGTGTTTATTGCCTGTGGTCCTGAAAAATT
  TCGCTATGCTCAGGATGATTTTTCTCTGGATGAAAATGAATGCCGAGTCATGAAGGGAAACCCATCAGCC
  ACAGCTGGCCCAAAGGCATCCCCAACACCTCAGAAGACTTCAGCCAAGAGCCCTGGTCCTATGCGCCGAA
  GCAAGTCTCCAGCTGACTCAGGTAACGACCAAGACGCAAACGGAACCTCCAGCAGCCAGCTCTCTACCCC
  CAAGTCTAAGCAGTCTCCCATCTCTACGCCCACCAGTCCTGGCAGCCTCCGGAAGCACAAGGACCTGTAC
  CTGCCTCTGTCCTTGGATGACTCGGACTCGCTTGGTGATTCCATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178152 unedited
  TTATAGGCGGCCGCGACATTCGCACGAGGGTTTTCTTTCTCTCAGCATCTCCACCCAACC
  AGCAGAAAACCGGTCTCTGAGGTTCCACCAAAATATGGAACTTGATTTTGGACACTTTGA
  CGAAAGAGATAAGACATCCAGGAACATGCGAGGCTCCCGGATGAATGGGTTGCCTAGCCC
  CACTCACAGCGCCCACTGTAGCTTCTACCGAACCAGAACCTTGCAGGCACTGAGTAATGA
  GAAGAAAGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGCTACTTCAAGGGGATTGT
  GTACGCTGTGTCCTCTGACCGTTTTCGCAGCTTTGACGCCTTGCTGGCTGACCTGACGCG
  ATCTCTGTCTGACAACATCAACCTGCCTCAGGGAGTGCGTTACATTTACACCATTGATGG
  ATCCAGGAAGATCGGAAGCATGGATGAACTGGAGGAAGGGGAAAGCTATGTCTGTTCCTC
  AGACAACTTCTTAAAAAGGTGGAGTACACCAAGAATGTCATCCCAACTGGTCTGNAACGT
  AAAACATCTGCCAATATGAAAGCCCCCCAGTCCTTGGCTAGCAGCAACAGTGCACAGGCC
  AGGGAGACAANGGACTTNTGTGCGCCCCAAGCTGGGTTACCATCATNNCGCAGTGGGGGG
  TGAAGCCTCNGAANGGCTGTNGGCNGTGTGCCTTCTGAACAAAGAAGACAGCCCNACTCT
  TTTNGAGCCAAGTNNCTCACTTGATATCACAAGAAAGCCATCAACTGGAGACCGGCGGGG
  GGTGGCAAAAAAACCTCTACCTCCTGGATGGAAAACAGGGNAACTGGGCTCATGATTCCC
  TTTGGGAGGAAAAAAAGGGAATATTCCCCCGGGGGGCGGAAAAAAATTCCCTATGCCAGA
  AGAAAATTTTTCTTGGTGAAAAGAATAGCCCGGTCTGAAAGGGAACCATCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_178152 unedited
  NAAAACTTTGNAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGGTA
  AATTTTCTTCATGTGTTTTTTAGACAAAAGAAAAATCACCAGTTTATAAAAAAGGAGCCT
  GAAGAGTTTGCTCCTGAAATACAGACATGAATTTTTATAAAAAGGATATTGATTTCTTGC
  TATAAAAAGAGACTGAAAGGTGTCAGACCTGAAAACAAATTCTCACAGTGTTTTCAGTGT
  TGGCTGTTAACAAGGCATTCAATAGAACAGAGATAGATGGATACCATACAGTTCATGTAA
  TCATGTGGTATGTTAGACAGCCTTCTAGAGGAATCAGGTATATGAAGTCCCTTACTCCAA
  ACTATTATCAATTGCTTATTGATTCCAAATTGCCTCAGTGGAGAGATTGACCAGGAATTC
  ACTGTGCTTTATAAACTCTTCGGAAATGTCTGCAGTATTTTCCAATTCTGTCTATTCTCT
  TAGCTAACCCAGCACCAGGATCCTAAGCATCTGGTGTCACAATTATAAGTTGTTGGTTTA
  TTTCTCCCCATTGACAGCCAGAATTGGTGCTGGACAACAAACCCGGGCCTCAATCANGAG
  TGAAGAGTGAGGCTGGNGAGCTGCCTTGCTGTCCTTCCAGGGTCCTCCACCATTCTTGAG
  ATCCAGAGAAAAGGGGCACTTGTGTTTGTCATTCTTGGATCTGCCAATGAGTTNTTTTTT
  TTCCCACACAAACATTTTAAGTGCTGTATATGTAAACAGCCCTCTACAAAAGAAGACTGT
  ATGGGCAAAATAGACAGGGCAATATTACGCCAGGCCCTAAACCTTCCCATGGAAGGTCAT
  GGACTAACACCATTTGCATCCCCGGAAGCCTGCCCAAAGGAAGGTTACAATCAATCCCCA
  AAATGGGAAACGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_178152
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178152.1, NP_835365.1
• RefSeq Size: 9069 bp
• RefSeq ORF: 1098 bp
• Locus ID: 1641
• UniProt ID: O43602
• Cytogenetics: Xq23
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (2) has an alternate 5' exon, resulting in a downstream AUG start codon, and has an additional segment in the 3' coding region, as compared to variant 1. The resulting isoform (b) has a shorter N-terminus and an extra internal segment compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.