OTUB1 (NM_017670) Human Untagged Clone

CAT#: SC108722

OTUB1 (untagged)-Human OTU domain, ubiquitin aldehyde binding 1 (OTUB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OTUB1
• Synonyms: HSPC263; OTB1; OTU1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017670 edited
  GAATTCGGCACGAGGGTCGGTAGTGCGGCGCTGTTTAAAGATGGCGGCGGAGGAACCTCA
  GCAGCAGAAGCAGGAGCCGCTGGGCAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGA
  TGAAGCCATCATGGCTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCT
  GGTGTCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGACAACAT
  CTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACATCCGCAAGACCAGGCC
  TGACGGCAACTGTTTCTATCGGGCTTTCGGATTCTCCCACTTGGAGGCACTGCTGGATGA
  CAGCAAGGAGTTGCAGCGGTTCAAGGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTC
  CCAGGGCTTCACTGAATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGA
  GCAGGTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCAGAGCAC
  CTCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCTACCTGCAGCGCGAGAG
  CAAGTTCTTCGAGCACTTCATCGAGGGTGGACGGACTGTCAAGGAGTTCTGCCAGCAGGA
  GGTGGAGCCCATGTGCAAGGAGAGCGACCACATCCACATCATTGCGCTGGCCCAGGCCCT
  CAGCGTGTCCATCCAGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCA
  CATCTTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACACTACGA
  TATCCTCTACAAATAGGGCTGGCTCCAGCCCGCTGCTGCCCTGCTGCCCCCCTCTGCCAG
  GCGCTAGACATGTACAGAGGTTTTTCTGTGGTTGTAAATGGTCCTATTTCACCCCCTTCT
  TCCTGTCACATGACCCCCCCCCATGTTTTATTAAAGGGGGTGCTGGTGGTGAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017670 unedited
  GGGGGTCAAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGTCGGTA
  GTGCGGCGCTGTTTAAAGAGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGG
  GCAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGGCTCAGCAGG
  ACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTGTCAGAGCGGCTGGAGCTCT
  CGGTCCTATACAAGGAGTATGCTGAAGATGACAACATCTATCAACAGAAGATCAAGGACC
  TCCACAAAAAGTACTCGTACATCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGG
  CTTTCGGATTCTCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCA
  AGGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTGAATTCACAA
  TTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAGGTGGAGAAGCAGACCTCTG
  TCGCCGACCTGCTGGCCTCCTTCAATGACCAGAGCACCTCCGACTACCTTGTGGTCTACC
  TGCGGCTGCTCACCTCGGGCTACCTGCAGCGCGAGAGCAAGTTCTTCGAGCACTTCATCG
  AGGGTGGACGGACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGA
  GCGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCCCAGTGGAGT
  ACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATCTTCCCCTGAGGCTCCGAGC
  CCCAGGTCTACCTTCTCTACCGGCCTGGACACTACGATATCCTCTACAAATAGGGCTGGC
  TTCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017670 unedited
  TATGGATCGCGGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TCACCACCAGCACCCCCTTTAATAAAACATGNGGGGGGGGTCATGTGACAGGAAAAAGGG
  GGTGAAATAGGACCATTTACAACCACAAAAAAACCTTTGTACATGTCTACCGCCTGGCAA
  AGGGGGGCAGCAGGGCAGCAGCGGGCTGGAGCCAGCCCTATTTGTAAAGGATATCGTAGT
  GTCCAGGCCGGTAAAAAAGGTAAACCTTGGGCTCGGAGCCCTCAGGGAAAATGTGCGGAT
  TGGTGGTGCCGCCCTCGCCGCGGCCCATGTACTCCACCTGGATGGACACGCTGAGGGCCT
  GGGCCAGCGCAATGATGTGGATGTGGCCGCTCTCCTTGCACATGGGCTCCACCTCCTGCT
  GGCAAAACTCCTTGACAGTCCGTCCACCCTCGATGAAGTGCTCGAAAAACTTGCTCTCGC
  GCTGAAGGTATCCCGAGGTGAACACCCCCAAGTAAACCACATGGTATTCGCAGGCGCTCT
  GGTCATTGAAGGAGGCCAGAAGGTCGGCAACAGAGGTCTGCTTTTTCACCTGTTCAATCA
  GGTCCATGAACCGGTTGCGCAAACTCCTAATTGTCAACTCAGTGAATCCCTGGGACACCT
  GTCCTTTCTTGCTTTTGCCAAACACACCCTTGAACCGTTGCCCTCCTTTCCTGAATTCCA
  ACAGCGCCCCCAATGGGGAAAATTCCCATACCCCTTAACAAAACAATCCCCTCCACGCCT
  GTCCCTTGCGTATTGACAACTCTTTTTGTAGGAAGTCCCTTCACCTCCCCGTCCAAAAAG
  CCCGCCCCTTTTCACACTATCTTCCTTCTATGCACACAAGATCCCCCCACCTTATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017670
• Insert Size: 1090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017670.1, NP_060140.1
• RefSeq Size: 1747 bp
• RefSeq ORF: 816 bp
• Locus ID: 55611
• UniProt ID: Q96FW1
• Cytogenetics: 11q13.1
• Protein Families: Protease
• Gene Summary: The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the predominant transcript.