ACSF2 (NM_025149) Human Untagged Clone

CAT#: SC108350

ACSF2 (untagged)-Human acyl-CoA synthetase family member 2 (ACSF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACSF2
• Synonyms: ACSMW; AVYV493
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108350 sequence for NM_025149 edited (data generated by NextGen Sequencing)
  ATGGCTGTCTACGTCGGGATGCTGCGCCTGGGGAGGCTGTGCGCCGGGAGCTCGGGGGTG
  CTGGGGGCCCGGGCCGCCCTCTCTCGGAGTTGGCAGGAAGCCAGGTTGCAGGGTGTCCGC
  TTCCTCAGTTCCAGAGAGGTGGATCGCATGGTCTCCACGCCCATCGGAGGCCTCAGCTAC
  GTTCAGGGGTGCACCAAAAAGCATCTTAACAGCAAGACTGTGGGCCAGTGCCTGGAGACC
  ACAGCACAGAGGGTCCCAGAACGAGAGGCCTTGGTCGTCCTCCATGAAGACGTCAGGTTG
  ACCTTTGCCCAACTCAAGGAGGAGGTGGACAAAGCTGCTTCTGGCCTCCTGAGCATTGGC
  CTCTGCAAAGGTGACCGGCTGGGCATGTGGGGACCTAACTCCTATGCATGGGTGCTCATG
  CAGTTGGCCACCGCCCAGGCGGGCATCATTCTGGTGTCTGTGAACCCAGCCTACCAGGCT
  ATGGAACTGGAGTACGTCCTCAAGAAGGTGGGCTGCAAGGCCCTTGTGTTCCCCAAGCAA
  TTCAAGACCCAGCAATACTACAACGTCCTGAAGCAGATCTGTCCAGAAGTGGAGAATGCC
  CAGCCAGGGGCCTTGAAGAGTCAGAGGCTCCCAGATCTGACCACAGTCATCTCGGTGGAT
  GCCCCTTTGCCGGGGACCCTGCTCCTGGATGAAGTGGTGGCGGCTGGCAGCACACGGCAG
  CATCTGGACCAGCTCCAATACAACCAGCAGTTCCTGTCCTGCCATGACCCCATCAACATC
  CAGTTCACCTCGGGGACAACAGGCAGCCCCAAGGGGGCCACCCTCTCCCACTACAACATT
  GTCAACAACTCCAACATTTTAGGAGAGCGCCTGAAACTGCATGAGAAGACACCAGAGCAG
  TTGCGGATGATCCTGCCCAACCCCCTGTACCATTGCCTGGGTTCCGTGGCAGGCACAATG
  ATGTGTCTGATGTACGGTGCCACCCTCATCCTGGCCTCTCCCATCTTCAATGGCAAGAAG
  GCACTGGAGGCCATCAGCAGAGAGAGAGGCACCTTCCTGTATGGTACCCCCACGATGTTC
  GTGGACATTCTGAACCAGCCAGACTTCTCCAGTTATGACATCTCGACCATGTGTGGAGGT
  GTCATTGCTGGGTCCCCTGCACCTCCAGAGTTGATCCGAGCCATCATCAACAAGATAAAT
  ATGAAGGACCTGGTGGTTGCTTATGGAACCACAGAGAACAGTCCCGTGACATTCGCGCAC
  TTCCCTGAGGACACTGTGGAGCAGAAGGCAGAAAGCGTGGGCAGAATTATGCCTCACACG
  GAGGCCCGGATCATGAACATGGAGGCAGGGACGCTGGCAAAGCTGAACACGCCCGGGGAG
  CTGTGCATCCGAGGGTACTGCGTCATGCTGGGCTACTGGGGTGAGCCTCAGAAGACAGAG
  GAAGCAGTGGATCAGGACAAGTGGTATTGGACAGGAGATGTCGCCACAATGAATGAGCAG
  GGCTTCTGCAAGATCGTGGGCCGCTCTAAGGATATGATCATCCGGGGTGGTGAGAACATC
  TACCCCGCAGAGCTCGAGGACTTCTTTCACACACACCCGAAGGTGCAGGAAGTGCAGGTG
  GTGGGAGTGAAGGACGATCGGATGGGGGAAGAGATTTGTGCCTGCATTCGGCTGAAGGAC
  GGGGAGGAGACCACGGTGGAGGAGATAAAAGCTTTCTGCAAAGGGAAGATCTCTCACTTC
  AAGATTCCGAAGTACATCGTGTTTGTCACAAACTACCCCCTCACCATTTCAGGAAAGATC
  CAGAAATTCAAACTTCGAGAGCAGATGGAACGACATCTAAATCTGTGA
  
  Clone variation with respect to NM_025149.4
  495 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025149 unedited
  GCACCAGGCAGGGCAAAGCGAGCCATGGCTGTCTACGTCGGGATGCTGCGCCTGGGGAGG
  CTGTGCGCCGGGAGCTCGGGGGTGCTGGGGGCCCGGGCCGCCCTCTCTCGGAGTTGGCAG
  GAAGCCAGGTTGCAGGGTGTCCGCTTCCTCAGTTCCAGAGAGGTGGATCGCATGGTCTCC
  ACGCCCATCGGAGGCCTCAGCTACGTTCAGGGGTGCACCAAAAAGCATCTTAACAGCAAG
  ACTGTGGGCCAGTGCCTGGAGACCACAGCACAGAGGGTCCCAGAACGAGAGGCCTTGGTC
  GTCCTCCATGAAGACGTCAGGTTGACCTTTGCCCAACTCAAGGAGGAGGTGGACAAAGCT
  GCTTCTGGCCTCCTGAGCATTGGCCTCTGCAAAGGTGACCGGCTGGGCATGTGGGGACCT
  AACTCCTATGCATGGGTGCTCATGCAGTTGGCCACCGCCCAGGCGGGCATCATTCTGGTG
  TCTGTGAACCCAGCCTACCAGGCTATGGAACTGGAGTACGTCCTCAAGAAGGTGGGCTGC
  AAGGCCCTTGTGTTCCCCAAGCAATTCAAGACCCAGCAATACTACAACGTCCTGAAGCAG
  ATCTGTCCAGAAGTGGAGAATGCCCAGCCAGGGGCCTTGAAGAGTCAGAGGCTCCCAGAT
  CTGACCACAGTCATCTCGGTGGATGCCCCTTTGCCGGGGACCCTGCTCCTGGATGAAGTG
  GTGGCGGCTGGCAGCACACGGCAGCATCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_025149 unedited
  NNAATTACTTGAACCGCGGCCCTATCTANGATCGATTTTTTTTTTTTTTTTTTTTTCCAT
  TCCTGTGAACTGGGAATCTATGTGCCTTTTGGAACCCAGCTGTTTTTATTTCTTGGGTCA
  AGGTACAACCTGCTTTATCTGGCCCTGCCTACGAGGGATGCCCCAGGTTCACCAATCAAA
  ACAAATAAAAACTAAAAATGAGGTTGGTTGGGGAAGGAAGGGGGCCTGGGAAATAGGGAG
  ATAATANTTAAAGAGTATGGGGTTTNTTTTTTAGGTGATGACATTATTTAAACTTGACTG
  TGGTGATAATTACCCTTATTTGTGAATTTCCTAAAAACCACTGAATTCTATACTTTAAAT
  GGTACATTGACCCAACACTTTAACAAACTGAGGCAGGAAGATTGCTTGAACCCATGACTT
  TTAAACTAACCTAAGCAACCAAGAAAGAACCTGTCACTATGTTAAAAACTTACTTAAGAA
  ATGGTGGCACATTCCTACAATCCACACTCCCCACGAGGCTCCGGTGGGAAGACTGCCTGA
  ACCCAATAGTTAAAGGCTGCACCGACATAAACTAACGCCCCCTACTATGAAGTCCCCGCA
  CCCCCACCCGCATGACACCATGAAGATCCAGGACCCCAGAACAAGGGCGAAGGCGGGACA
  GAACCCGGCAACAACGGAAACTACACCTCAATCCCCCCTGGTTACACCTATATCAGAAAG
  AACACTTTAATCGAAAGCCAACCCCCCGAAGATACGCCCAAAACAAATAAAGACAACACT
  CCCCCTCAACACGAAAAAAGTGAGGCCAAGGAGCACATGCCGCATCGACCAGAAACCACC
  AAACAATAGGACGACATAAGACCGCGGAGAAGCACTGACGGAACGGGGCTGACAGAAACA
  ACACCGAAACAACTGATAAAAGCAACGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025149
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025149.3, NP_079425.2
• RefSeq Size: 2205 bp
• RefSeq ORF: 1848 bp
• Locus ID: 80221
• UniProt ID: Q96CM8
• Cytogenetics: 17q21.33
• Domains: AMP-binding
• Gene Summary: Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.