Slit2 (NM_022632) Rat Tagged ORF Clone

CAT#: RR217651

Slit2 (myc-DDK-tagged) - Rat slit homolog 2 (Drosophila) (Slit2)

Product Images

Circular map for RR217651

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slit2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217651 representing NM_022632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCTCTGGCGTTAGTGTTGTCGATCTTGAACCAGGTGG
  CGCCTCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACAGTGGACTGTCATGGGCTGGCACTGCG
  CAGTGTGCCCAGGAATATCCCCCGCAACACGGAGAGACTGGATTTGAATGGAAATAACATCACAAGGATC
  ACGAAGACAGATTTTGCGGGTCTCAGACACCTCAGAGTTCTTCAGCTCATGGAGAACAAGATCAGCACCA
  TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTAGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT
  GTTTCCTGAGCTGCTGTTTCTTGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAGATTCAA
  GCAATTCCAAGGAAGGCTTTCCGTGGTGCAGTTGACATTAAAAATCTGCAGTTGGATTACAACCAGATCA
  GCTGCATTGAAGATGGGGCATTCCGAGCTCTGCGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT
  TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCCAACAAC
  CTATACTGCGACTGCCACCTGGCCTGGCTCTCGGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA
  CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCATAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG
  CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCGATTGCT
  TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG
  AGACCATCACAGAAATACGTTTGGAACAGAACTCCATAAGGGTCATCCCTCCAGGAGCATTCTCACCATA
  CAAAAAGCTTCGACGACTAGACCTGAGTAATAACCAGATCTCGGAACTTGCTCCAGATGCCTTCCAAGGA
  CTGCGTTCTCTGAATTCCCTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTTGAAG
  GACTGTTTTCCTTACAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGACCTGCACAACTTGAACCTTCTCTCCTTATACGACAATAAGCTTCAGACTGTTGCCAAGGGCACCTTC
  TCAGCTCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT
  GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGTCCCCGCCGCCT
  GGCTAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCA
  AAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAAAAATGTCGCTGTGAAGGGACCACAGTAG
  ACTGCTCCAATCAAAAACTCAACAAAATCCCAGACCATATTCCCCAGTACACAGCAGAGCTGCGTCTCAA
  TAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAACTTCCTCAATTGCGTAAAATCAAC
  CTTAGCAACAATAAGATCACTGATATCGAGGAGGGGGCATTCGAAGGTGCGTCTGGTGTGAATGAGATTC
  TGCTTACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAAAGGATTGGAGAGCCTCAAAACATT
  GATGCTGAGAAGTAATCGAATAAGCTGTGTGGGAAACGACAGTTTCACAGGACTCGGTTCTGTGCGTCTG
  CTCTCTTTATATGACAATCAAATTACCACAGTTGCACCAGGAGCATTTGGTACTCTCCATTCATTATCTA
  CACTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGCATGGCTTGGAGAATGGCTCAGAAG
  GAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTCTTGAAGGAAATACCAATCCAGGAT
  GTAGCCATTCAGGACTTCACCTGTGATGACGGAAACGATGATAATAGCTGCTCTCCACTCTCCCGTTGTC
  CTTCGGAATGTACTTGCTTGGATACAGTAGTACGATGTAGCAACAAGGGCTTGAAGGTCTTACCTAAAGG
  CATTCCAAGAGATGTCACAGAACTGTATCTGGATGGGAACCAGTTTACACTGGTCCCGAAGGAACTCTCC
  AACTACAAACATTTAACACTTATAGACTTAAGTAACAACAGAATAAGCACCCTTTCCAACCAAAGCTTCA
  GCAACATGACCCAACTTCTCACCTTAATTCTCAGTTACAACCGTCTGAGATGTATCCCTCCACGGACCTT
  TGATGGATTGAAATCTCTTCGTTTACTGTCTCTACATGGAAATGACATTTCTGTCGTGCCTGAAGGTGCC
  TTTGGTGACCTTTCAGCCTTGTCACACTTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGC
  AGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAATTGCCCGCTGTGCCGGTCCCGGAGA
  AATGGCAGATAAATTGTTACTCACAACTCCCTCCAAAAAATTTACATGTCAAGGTCCTGTGGATGTTACT
  ATTCAAGCCAAGTGTAACCCCTGCTTGTCAAATCCATGTAAAAATGATGGCACCTGTAACAATGACCCGG
  TGGATTTTTATCGATGCACCTGCCCATATGGTTTCAAGGGCCAGGACTGTGATGTCCCCATTCATGCCTG
  TATCAGTAATCCATGTAAACATGGAGGAACTTGCCACTTAAAAGAAGGAGAGAATGATGGATTCTGGTGT
  ACTTGTGCTGATGGGTTTGAAGGAGAAAGCTGTGACATCAATATTGATGATTGCGAAGATAATGATTGTG
  AAAATAATTCTACATGCGTTGATGGAATTAACAACTACACGTGTCTTTGCCCACCGGAATACACAGGCGA
  ACTGTGTGAGGAAAAACTGGACTTCTGTGCACAAGACCTGAATCCCTGCCAGCATGACTCCAAGTGCATC
  CTGACGCCAAAGGGATTCAAGTGTGACTGCACTCCGGGATACATTGGTGAGCACTGTGACATCGACTTTG
  ATGACTGCCAAGATAACAAGTGCAAAAACGGTGCTCATTGCACAGATGCAGTGAACGGATACACATGTGT
  CTGTCCTGAAGGCTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCC
  TGTGATAATTTTGATTGTCAGAATGGAGCCCAGTGTATCATCAGGGTGAATGAACCAATATGCCAGTGTT
  TGCCTGGCTACTTGGGAGAGAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCT
  TCAGATTCCTTCAGCCAAGGTTCGACCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGC
  GGCATCCTCTTGTACAAGGGTGACAAGGACCACATTGCTGTGGAACTCTATCGAGGGCGAGTTCGAGCCA
  GCTATGACACCGGCTCTCACCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCA
  CATTGTAGAGCTACTGACCCTGGATTCGAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAATCATC
  ACCAATTTGTCAAAACAATCTACTCTGAATTTCGACTCTCCACTTTACGTAGGAGGTATGCCTGGGAAAA
  ATAACGTGGCTTCGCTGCGCCAGGCCCCTGGGCAGAACGGCACCAGCTTCCATGGCTGTATCCGGAACCT
  TTACATTAACAGTGAACTGCAGGACTTCCGGAAAGTGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAA
  CCATGCCACAAGAAAGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAAT
  GTGAGGAAGGGTGGATGGGGCCCCTCTGTGACCAGAGAACCAATGATCCCTGTCTCGGAAACAAATGTGT
  ACATGGGACCTGCTTGCCCATCAACGCCTTCTCCTACAGCTGCAAGTGCCTGGAGGGCCACGGCGGGGTC
  CTCTGTGATGAAGAAGAAGATCTGTTTAACCCCTGCCAGGTGATCAAGTGCAAGCACGGGAAGTGCAGGC
  TCTCTGGGCTCGGGCAGCCCTATTGTGAATGCAGCAGTGGATTCACCGGGGACAGCTGTGACAGAGAAAT
  TTCTTGTCGAGGGGAACGGATAAGGGATTATTACCAAAAGCAGCAGGGTTACGCTGCCTGTCAAACGACT
  AAGAAAGTATCTCGCTTGGAGTGCAGAGGCGGGTGTGCTGGGGGGCAGTGCTGTGGACCTCTGAGAAGCA
  AGAGGCGGAAATACTCTTTCGAATGCACAGATGGATCTTCATTTGTGGACGAGGTCGAGAAGGTGGTGAA
  GTGCGGCTGCACGAGATGTGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217651 representing NM_022632
  Red=Cloning site Green=Tags(s)
  
  MSGIGWQTLSLSLALVLSILNQVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRI
  TKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQ
  AIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNN
  LYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPIA
  CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQG
  LRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTF
  SALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRS
  KLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKIN
  LSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFTGLGSVRL
  LSLYDNQITTVAPGAFGTLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQD
  VAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELS
  NYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGA
  FGDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVT
  IQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENDGFWC
  TCADGFEGESCDINIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCI
  LTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSP
  CDNFDCQNGAQCIIRVNEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDS
  GILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKII
  TNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCE
  PCHKKVCAHGTCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGV
  LCDEEEDLFNPCQVIKCKHGKCRLSGLGQPYCECSSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTT
  KKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022632
• ORF Size: 4575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022632.2, NP_072154.2
• RefSeq Size: 7135 bp
• RefSeq ORF: 4578 bp
• Locus ID: 360272
• Cytogenetics: 14q11
• MW: 169.1 kDa
• Gene Summary: acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [RGD, Feb 2006]