Fnip2 (NM_001271167) Rat Tagged ORF Clone

CAT#: RR217484

Fnip2 (myc-DDK-tagged) - Rat folliculin interacting protein 2 (Fnip2)

Product Images

Circular map for RR217484

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fnip2
• Synonyms: RGD1562174
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217484 representing NM_001271167
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGACCCTGCTCCAGAAGCTCTTCAACAAAAGGGGCGGCGGCGCAGCGTCCGCCCAGGCCCGGC
  CGCCCAAGGAGGAGCCGGCCTTCAGTTGGTCGTGTTCGGAGTTTGATTTGAGTGACATTCGCCTGATAGT
  GTACCAGGACTGCGAGAGGAGAGGCAGACAAGTCATGTTTGACTCCAGAGCCGTGCAGAAGGTGGAAGAG
  ACAGCAGCTCAGAAAGCAGAGGACGTCCCTATTAAAATGTCAGCGAGATGCTGTCAGGAAAGCGGCAGTG
  TCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCTCTTCTCAGAGCTCTGGGGGATCTTCACAGCACGC
  TAAGGAGCAGCTTCCAAAGTACCAATACACGAGACCAGCATCTGATGTCAACATGCTTGGGGAAATGATG
  TTCGGCTCTGTCGCAATGAGTTACAAAGGCTCCACCCTGAAGATACACTACATACGATCTCCTCCACAAC
  TGATGATTAGTAAAGTCTTCTCTGCTAGAATGGGCAGCTTCTGTGGAAGTACAAATAATCTGCAGGACAG
  CTTCGAATACATCAACCAAGATCCTCAGGCTGGAAAACTGAACACAAATCAGAACAGTTTGGGTCCTTGT
  CGTACTGGAAGTAACCTAGCACACAGCACACCTGTTGATATGCCAAGCAGAGGGCAGAATGAAGACAGGG
  ACAGTGGCATTGCTCGATCAGCTTCACTGAGCAGCCTGCTGATCACACCCTTCCCATCTCCGAGCTCCTC
  TACATCCTCCTCCAGCAGTTACCAGCGCCGCTGGCTTCGAAGTCAGACCACGAGTTTGGAAAATGGCATC
  TTTCCAAGGAGGTCAACAGATGAAACATTTAGCTTGGCTGAAGAAACCTGTAGCTCTAATCCATCCATAG
  TTAGACGGAAGAAAATTGCCATCAGTATCATCTTTTCCTTGTGTGAGAGAGAGGCCGCACAGCGGGATTT
  CCAGGACTTCTTTTTCTCCCACTTCCCCCTGTTTGAATCTCACATGAACAGGCTGAAGAGTGCAATTGAA
  AAGGCCATGATCTCCTGTAGGAAGATCTCTGAATCAAGTCTCCGTGTCCAGTTTTATGTGAGCCGTCTGA
  TGGAAGCCCTGGGAGAATTCAGAGGGACTATCTGGAACTTATATTCTGTTCCAAGGATAGCTGAACCAGT
  GTGGCTTACCATGATGTCCAACACCTTGGAAAAAAACCAACTCTGCCAGCGCTTTCTCAAGGAATTTATA
  CTTCTGATTGAACAGGTCAACAAAAACCAGTTTTTCGCTGCCTTACTGACTGCGGTGTTAACCTACCACC
  TGGCCTGGGTACCAACTGTCATGCCTGTTGACCATCCTCCCATTAAAACCTTCTCGGAGAAGCGCACCTC
  TCAGTCAGTGAACATGCTGGCCAAAACACATCCATATAATCCTCTCTGGGCACAGCTGGGTGATCTTTAT
  GGAGCTATAGGCTCTCCAGTGAGACTGACTCGCACCGTGGTGATTGGGAAGCAGAAGGATTTGGTCCAGC
  GCATTCTTTATGTTCTAACCTACTTTCTCCGTTGCTCTGAACTGCAAGAGAATCAACTGACCTGGAGTGG
  GAATCACAGTGACGATGACCAGGTTATAAATGGGAGCAAGATTGTAACTGCCTTGGAAAAAGGAGAGGTA
  GAAGAGTCTGAGTATGTGGTAGTTACAGTGAGCAATGAGCCTGCTCTGGTACCACCAGTCCTACCACCAG
  GGACATCTGAGAGGACGAGCCCTGAGCCTACAGTAGTAGCTGAGATCCCAGAAGGCATTAATACCGAACT
  GGGCCACAAACCTGAAAGAAACAGATGCAAGAGGCCAGAGCAATATTCTGAGGCCAGCAGCATGGGGTTC
  CAAGAGGCCGAAACTGACAATTCATGGACACCTCGAGGCATGTTCTGTGAGGACAAACAGAATGACCAAC
  AGGTAGCCCAGGATTGTTCTTCAGGACCTCCCAGCTGTGAGGTGCCCAGGGTAAAAAGGAGGATGGACCA
  ACAAACTTTCCATGAAGAGCTGCATGGCGAGATGCTAAAGAAACGAGCAGAGCGCTCAGCAGCCTGGCCC
  TGCCCTGACAGACATTCCCAGGAGGGTCCTCCTGTTGAAAAGGTCACTTTCCACATTGGAAGTTCCATCT
  CTCCAGAGTCTGACTTTGAAAGCCGCACCAAAAGAATGGAGGAGCGGTTAAAGGCCTGTGGACATTTTCA
  TGGGGCAAGTGCCTCTGCCAGGTCTAGCATGGACACTGGCCTGACTCAAGACCAGCAGGCCTCTGGGTGC
  TCCTTCAAAGCTGACTTTCAGAAGGACATCACACCTCAGGACCACTGTTTAGGGGAAGAAGGTGTCTCTG
  AGAACAGAGGCCTCCGAGCCAACATCCGAGCCAACATGGCACATGCCGTGGGACAGCTGAGCCAAGCTGA
  TGGCCCCCTTGCACCTTCCACTTGTACAGCAGAAAGTGGCTGGAGACAGCTGGAACAGACTAGAGATGTG
  CAATTAAAAGGCAACAAAGGACCTTCATCAGAGCCTGTTCCAAACAGATGTAGACAGCAGGGTGACCTGC
  TGATTGCTGCAGATGTCCCCTATGGGGATGCCAGTGGTAAGGCCAACTACAGGAGCGAAGGAGACATTCC
  CAGGAATGAAAGCTTGGATAGTGCTCTTGGAGACAGTGACGATGAAGCGTGTGTTTTAGCCCTGCTAGAA
  CTTGGTCACAATTGTGACAGGACTGAAGGGTCCTTTGAAGTGGAACTGCCTCTACCAAGGTCTCAGAGTA
  CCAGCAAGCCAAATGTTAGAAATTTTGGCCGCTCACTTCTGGCAGGTTACTGCGCCACGTATATGCCTGA
  CTTGGTGCTGCACGGAACCAGCAGCGATGAGAAGCTGAAGCAGTGCCTGGCAGCCGATCTGGTCCACACG
  GTGCATCACCCAGTGCTCGATGAGCCCATAGCCGAAGCCGTCTGCATCATTGCAGACACTGACAAGTGGA
  CTGTCCAGGTGGCCACAAGTCAGAGGAAAGTGACGGACGCCATGAAGCTGGGCCAGGATGTCCTGGTCTC
  TAATCAGGTGTCCAGTTTACTCCAGTCCATCTTACAGCTCCATAAGCTTCACCTCCCCGCTGATTTTTGC
  ATCATGCATCTGGAAGACAGACTACAGGAGATGTACCTCAAAAGTAAAATGCTTTCAGAGTATCTCCGGG
  GACACACACGTGTGCACGTGAAAGAGCTGAGCGTGGTGCTGGGGATTGAATCCAGTGACCTGCCTCTGCT
  GACTGCCATCGCCAGCACTCACTCTCCGTATGTGGCTCAGATCCTCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217484 representing NM_001271167
  Red=Cloning site Green=Tags(s)
  
  MAPTLLQKLFNKRGGGAASAQARPPKEEPAFSWSCSEFDLSDIRLIVYQDCERRGRQVMFDSRAVQKVEE
  TAAQKAEDVPIKMSARCCQESGSVSSSSSSSSNSSSSQSSGGSSQHAKEQLPKYQYTRPASDVNMLGEMM
  FGSVAMSYKGSTLKIHYIRSPPQLMISKVFSARMGSFCGSTNNLQDSFEYINQDPQAGKLNTNQNSLGPC
  RTGSNLAHSTPVDMPSRGQNEDRDSGIARSASLSSLLITPFPSPSSSTSSSSSYQRRWLRSQTTSLENGI
  FPRRSTDETFSLAEETCSSNPSIVRRKKIAISIIFSLCEREAAQRDFQDFFFSHFPLFESHMNRLKSAIE
  KAMISCRKISESSLRVQFYVSRLMEALGEFRGTIWNLYSVPRIAEPVWLTMMSNTLEKNQLCQRFLKEFI
  LLIEQVNKNQFFAALLTAVLTYHLAWVPTVMPVDHPPIKTFSEKRTSQSVNMLAKTHPYNPLWAQLGDLY
  GAIGSPVRLTRTVVIGKQKDLVQRILYVLTYFLRCSELQENQLTWSGNHSDDDQVINGSKIVTALEKGEV
  EESEYVVVTVSNEPALVPPVLPPGTSERTSPEPTVVAEIPEGINTELGHKPERNRCKRPEQYSEASSMGF
  QEAETDNSWTPRGMFCEDKQNDQQVAQDCSSGPPSCEVPRVKRRMDQQTFHEELHGEMLKKRAERSAAWP
  CPDRHSQEGPPVEKVTFHIGSSISPESDFESRTKRMEERLKACGHFHGASASARSSMDTGLTQDQQASGC
  SFKADFQKDITPQDHCLGEEGVSENRGLRANIRANMAHAVGQLSQADGPLAPSTCTAESGWRQLEQTRDV
  QLKGNKGPSSEPVPNRCRQQGDLLIAADVPYGDASGKANYRSEGDIPRNESLDSALGDSDDEACVLALLE
  LGHNCDRTEGSFEVELPLPRSQSTSKPNVRNFGRSLLAGYCATYMPDLVLHGTSSDEKLKQCLAADLVHT
  VHHPVLDEPIAEAVCIIADTDKWTVQVATSQRKVTDAMKLGQDVLVSNQVSSLLQSILQLHKLHLPADFC
  IMHLEDRLQEMYLKSKMLSEYLRGHTRVHVKELSVVLGIESSDLPLLTAIASTHSPYVAQILL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271167
• ORF Size: 3339 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271167.1, NP_001258096.1
• RefSeq Size: 4298 bp
• RefSeq ORF: 3342 bp
• Locus ID: 310538
• Cytogenetics: 2q33
• MW: 123.4 kDa