Vav3 (NM_001191714) Rat Tagged ORF Clone

CAT#: RR217259

Vav3 (myc-DDK-tagged) - Rat vav 3 guanine nucleotide exchange factor (Vav3)

Product Images

Circular map for RR217259

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vav3
• Synonyms: RGD1565941
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217259 representing NM_001191714
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGTGGAAGCAGTGCGCTCAGTGGCTCATCCACTGCAAAGTGCTGCCCGCCAACCACCGGGTGA
  CCTGGGACTCGGCGCAGGTGTTCGACCTGGCGCAGACCCTCCGCGATGGAGTCCTGCTCTGCCAGCTGCT
  CAACAACCTGCGGCCACACTCCATCAATCTGAAGGAGATCAACCTGAGACCCCAGATGTCCCAGTTTCTC
  TGTCTGAAGAATATCCGGACATTTCTGGCCGCCTGCTGTGACACGTTCGGAATGAAGAAAAGTGAGCTTT
  TTGAGGCATTTGACCTATTCGATGTTCGTGACTTTGGAAAGGTTATAGAAACATTATCTCGCCTATCCCG
  AACACCAATAGCATTGGCCACAGGAATCAGGCCATTCCCCACGGAAGAAAGTGTAAATGATGAAGACATC
  TACAAAGGCCTTCCTGACCTGATAGATGAAACCCGTGTGGAGGACGAAGAAGACCTCTATGACTGTGTGT
  ACGGGGAGGATGAAGGCGGAGAAGTCTATGAAGATTTGATGAAGGCGGAGGAGGCTCACCAAGCTAAATC
  ACAAGAAAATGATATACGGAGTTGTTGCCTAGCAGAAATTAGGCAGACAGAAGAAAAATACACAGAAACT
  TTGGAGTCAATAGAAAAATATTTCATGGCACCATTGAAAAGATTTCTGACAGCGGCGGAATTTGATTCAG
  TATTTATCAACATTCCTGACCTTGTAAAAGTTCATCGGAGCTTAATGCAAGAGATTCACGATTCCATTGT
  AAATAAAGATGACCATAACTTGTATCAAGTTTTTATTAACTACAAGGAAAGATTGGTTATTTATGGACAG
  TACTGCAGTGGAGTGGAGTCAGCCATCTCTAGCCTAGACTACATCTCTAAGACAAAAGAAGATGTCAGAC
  TGAAACTAGAGGAATGTTCAAAGAGAGCCAATAATGGGAAATTTACTCTGCGTGATTTGCTGGTAGTTCC
  CATGCAGCGGGTGCTGAAATATCACCTCCTCCTTCAGGAGCTGGTTAAACACACCCATGATCCCATGGAG
  AAGTCGAACCTGAAGCTGGCTCTGGATGCAATGAAGGACTTGGCACAATATGTGAATGAAGTAAAAAGAG
  ATAATGAGACCCTTCGTGAAATTAAGCAGTTTCAGCTATCTATAGAAAATTTGAACCAGCCCGTTTTGCT
  CTTTGGGCGACCGCAGGGAGACGGTGAAATCCGAATAACCACACTGGACAAGCATACGAAACAAGAAAGG
  CATATCTTCTTATTCGATCTGGCAGTGATTGTCTGTAAAAGGAAAGGGGATAACTACGAGATGAAGGAAA
  TAATAGATCTCCAGCAGTACAAGATCGCCAACAACCCTACCACGGATAAAGAGAATAAAAAGTGGTCTTA
  TGGCTTCTACCTCATCCATACCCAAGGACAAAATGGGCTGGAATTTTATTGCAAAACAAAAGATTTAAAG
  AAAAAATGGCTGGAACAGTTTGAAATGGCTTTATCCAACATAAGGCCGGACTATGCAGACTCCAATTTCC
  ATGACTTCAAGATGCACACATTCACTCGGGTGACATCCTGTAGGGTCTGCCAAATGCTCCTGAGGGGGAC
  ATTTTATCAAGGCTACTTATGTTTTAAGTGTGGAGCAAAAGCGCATAAAGAATGTTTGGGAAGAGTAGAC
  AATTGTGGCAGAGTTAATTCCGTTGAACAAGGGCCATTCAAACCACCGGAGAAGCGGACCAATGGACTTC
  GGAGAGCTTCCAGACAGGTGGATCCAGGCTTGCCGAAGATGCGGGTCATTAGGAACTACACTGGCACCCC
  GCCCCCAGCTCTTCACGAAGGACCACCGTTGCATATCCAGGCAGGGGACACTGTTGAACTTCTGAGAGGA
  GAGGCACACACTGTTTTCTGGCAGGGACGAAACTTAGCTTCAGGAGAGGTTGGATTTTTCCCAAGCGATG
  CTGTCAAGCCATCTCCATGTGTACCTAAACCAGTAGATTATTCTTGCCAACCCTGGTATGCTGGCCCAAT
  GGAAAGATTGCAGGCAGAAACTGAACTTATTAACAGGGTGAATAGCACTTACCTTGTAAGGCTCAGGACC
  AAAGAGTCTGGAGAATATGCAATTAGCATTAAGTACAATAATGAAGCAAAACACATCAAGATTTTAACAA
  GAGATGGCTTTTTTCACATTGCAGAAAATAGAAAATTTAAAAGCTTAATGGAACTTGTGGAGTACTACAA
  GCACCATTCTCTTAAAGAAGGGTTCAGGACCTTAGATACGACGCTACAGTTTCCATACAAGGAGCCTGAA
  CACGCATCTGGACAGAGGGGTAATAGAACAGGCAACAGCTTGCTAAGTCCCAAAGTTCTGGGCATTGCCA
  TCGCTCGGTATGACTTCTGTGCAAGAGATATGCGGGAGCTCTCCCTATTGAAAGGAGACATGGTGAAGAT
  TTACACAAAGATGAGTGCAAATGGCTGGTGGAGAGGAGAAGTAAATGGCAGGGTGGGCTGGTTTCCATCC
  ACGTATGTGGAAGAAGACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217259 representing NM_001191714
  Red=Cloning site Green=Tags(s)
  
  MEPWKQCAQWLIHCKVLPANHRVTWDSAQVFDLAQTLRDGVLLCQLLNNLRPHSINLKEINLRPQMSQFL
  CLKNIRTFLAACCDTFGMKKSELFEAFDLFDVRDFGKVIETLSRLSRTPIALATGIRPFPTEESVNDEDI
  YKGLPDLIDETRVEDEEDLYDCVYGEDEGGEVYEDLMKAEEAHQAKSQENDIRSCCLAEIRQTEEKYTET
  LESIEKYFMAPLKRFLTAAEFDSVFINIPDLVKVHRSLMQEIHDSIVNKDDHNLYQVFINYKERLVIYGQ
  YCSGVESAISSLDYISKTKEDVRLKLEECSKRANNGKFTLRDLLVVPMQRVLKYHLLLQELVKHTHDPME
  KSNLKLALDAMKDLAQYVNEVKRDNETLREIKQFQLSIENLNQPVLLFGRPQGDGEIRITTLDKHTKQER
  HIFLFDLAVIVCKRKGDNYEMKEIIDLQQYKIANNPTTDKENKKWSYGFYLIHTQGQNGLEFYCKTKDLK
  KKWLEQFEMALSNIRPDYADSNFHDFKMHTFTRVTSCRVCQMLLRGTFYQGYLCFKCGAKAHKECLGRVD
  NCGRVNSVEQGPFKPPEKRTNGLRRASRQVDPGLPKMRVIRNYTGTPPPALHEGPPLHIQAGDTVELLRG
  EAHTVFWQGRNLASGEVGFFPSDAVKPSPCVPKPVDYSCQPWYAGPMERLQAETELINRVNSTYLVRLRT
  KESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPE
  HASGQRGNRTGNSLLSPKVLGIAIARYDFCARDMRELSLLKGDMVKIYTKMSANGWWRGEVNGRVGWFPS
  TYVEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191714
• ORF Size: 2541 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191714.1, NP_001178643.1
• RefSeq Size: 4642 bp
• RefSeq ORF: 2544 bp
• Locus ID: 295378
• Cytogenetics: 2q41
• MW: 98 kDa