Nfatc1 (NM_001244933) Rat Tagged ORF Clone

CAT#: RR217241

Nfatc1 (myc-DDK-tagged) - Rat nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (Nfatc1)

Product Images

Circular map for RR217241

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfatc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217241 representing NM_001244933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGTACCAGCTTTCCAGTCCCTTCCAAGTTTCCACTCGGCCCTCCGGCCGCAGTCTGCGGGAGCG
  GAGAAACTTTGCGGCCCGCGCCGCTCGCCGGCGGCACCATGAAGGCGGCCGAGGAAGAACACTACAGTTA
  TGCGTCCCCTAGCGTCACCTCAACTCTGCCCCTTCCCACAGCACACTCCGCCTTGCCAGCGGCATGCCAT
  GACCTCCAGACGTCCACCCCGGGCATCTCAGCTGTTCCTTCGGCCAATCACCCCTCTGGTTATGGGGGCG
  CTGTGGACAGTGGGCCTTCAGGATACTTCCTGTCCTCCGGCAACACGAGACCCAATGGGGCCCCGACTCT
  CGAGAGTCCTCGAATCGAGATCACCTCCTATCTGGGCCTTCACCACGGCAGCAACCAATTTTTCCACGAT
  GTGGAGGTGGAAGACGTACTTCCTAGCTGCAAGCGGTCACCATCTACAGCAACCCTGCACCTGCCCAGTC
  TGGAAGCCTACAGAGACCCCTCCTGCCTGAGCCCAGCCAGCAGCCTGTCCTCCAGGAGCTGTAACTCTGA
  GGCGTCCTCCTATGAGTCCAACTATTCATACCCGTACGCATCCCCCCAGACCTCTCCATGGCAGTCACCC
  TGTGTGTCTCCCAAGACCACGGACCCAGAGGAGGGCTTTCCCCGAAGCCTGGGTGCCTGCCACTTGCTGG
  GATCGCCAAGGCACTCCCCATCCACCTCTCCTCGTGCAAGCGTCACCGAGGAGAGCTGGCTCGGTGCCCG
  TGGCTCCCGGCCCACATCCCCCTGCAACAAGCGCAAATACAGTCTCAACGGCAGGCAGCCCTCCTGCTCA
  CCCCACCACTCGCCCACACCATCCCCCCATGGCTCCCCTCGGGTCAGCGTGACGGAAGACACCTGGCTTG
  GTAACACCACCCAGTACACCAGCTCTGCTATTGTGGCAGCCATCAACGCCCTGACCACTGATAGCACTCT
  GGACCTGGGTGACGGTGTCTCTATCAAGTCTCGAAAGACAGCCCTGGAGCACGTGCCCTCTGTGGCCCTC
  AAGGTAGAGCCGGCTGGGGAAGACCTGGGCACCACACCACCCACTTCTGACTTCCCACCCGAGGAGTTCG
  CCTTCCAGCACCTTCGGAAGGGTGCCTTTTGTGAGCAGTATCTGTCGGTGCCACAGGCCTCGTATCAGTG
  GGCAAAGCCCAAGTCTCTATCCCCAACGTCATACATGAGCCCATCCCTGCCTGCCCTTGACTGGCAGCTG
  CCGTCGCACTCTGGTCCGTATGAACTCCGGATCGAGGTGCAACCCAAGTCTCACCACAGGGCTCACTATG
  AGACGGAAGGCAGCCGGGGAGCTGTGAAGGCTTCTGCTGGAGGACACCCTGTCGTGCAGCTACATGGTTA
  CTTGGAAAACGAGCCTCTCACGCTCCAGCTGTTCATTGGGACGGCTGACGACCGCCTGCTACGGCCCCAT
  GCCTTCTACCAGGTCCACCGGATCACGGGGAAGACTGTCTCCACCACCAGCCACGAGGCTATCCTGTCCA
  ACACCAAAGTCCTGGAGATCCCACTGCTTCCAGAAAATAACATGCGAGCCATCATCGACTGTGCTGGGAT
  CCTGAAGCTCAGAAACTCTGATATCGAGCTGAGGAAAGGGGAGACAGACATCGGGAGAAAGAACACCAGG
  GTGCGGCTGGTCTTCCGAGTCCACATCCCCCAGCCCAGCGGCCGGACGCTGTCTCTCCAGGTGGCCTCAA
  ACCCGATCGAGTGCTCCCAGCGGTCGGCCCAGGAACTGCCCCTCGTGGAGAAGCAGAGCACAGACAGCTA
  CCCGGTCATTGGAGGGAAGAAGATGGTGTTGTCTGGCCATAACTTTCTGCAAGACTCCAAGGTCATCTTC
  GTGGAGAAGGCCCCAGATGGCCACCATGTCTGGGAGATGGAAGCGAAAACTGACCGAGACCTGTGCAAGC
  CAAATTCCCTGGTGCTGGAGATACCACCTTTCCGCAACCAGAGAATAACCAGCCCCGTCCAAGTCAGCTT
  CTATGTCTGCAACGGGAAGCGGAAGAGAAGCCAGTACCAGCATTTCACGTACCTTCCTGCCAATGTTCCA
  ATTATAAAGACAGAACCCACGGACGACTTTGAGCCAGCTCTGACCTGTGGACCAATGAGCCAGGGACTTA
  GCCCTCTGCCGAGGCCTTACTATAGCCAACAACTCACCGCGCCTCCTGACCCTGGCTCCTGCCTTGTGGC
  CGGCTTTGCCCCCTGCTCACAGAGGAACACGCCGATGCCCACGTCTCCCAATGCAAGCCCAAAGCTCCAC
  GACCTTTCCTCTCCCGCCTACGCCAAGGGCCTCGCCAACCCAGGCCACAGTGGTCACCTTGGACTTCAGC
  CACCAGCTTCAGAGGCCCCCACCATGCAGGAATTGCCGAGACCCATGGCCGTCCAGCCCAACTCGCCTGA
  GCAGCCCACATCCGTCAGGCTACAGCCGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217241 representing NM_001244933
  Red=Cloning site Green=Tags(s)
  
  MPSTSFPVPSKFPLGPPAAVCGSGETLRPAPLAGGTMKAAEEEHYSYASPSVTSTLPLPTAHSALPAACH
  DLQTSTPGISAVPSANHPSGYGGAVDSGPSGYFLSSGNTRPNGAPTLESPRIEITSYLGLHHGSNQFFHD
  VEVEDVLPSCKRSPSTATLHLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSP
  CVSPKTTDPEEGFPRSLGACHLLGSPRHSPSTSPRASVTEESWLGARGSRPTSPCNKRKYSLNGRQPSCS
  PHHSPTPSPHGSPRVSVTEDTWLGNTTQYTSSAIVAAINALTTDSTLDLGDGVSIKSRKTALEHVPSVAL
  KVEPAGEDLGTTPPTSDFPPEEFAFQHLRKGAFCEQYLSVPQASYQWAKPKSLSPTSYMSPSLPALDWQL
  PSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPVVQLHGYLENEPLTLQLFIGTADDRLLRPH
  AFYQVHRITGKTVSTTSHEAILSNTKVLEIPLLPENNMRAIIDCAGILKLRNSDIELRKGETDIGRKNTR
  VRLVFRVHIPQPSGRTLSLQVASNPIECSQRSAQELPLVEKQSTDSYPVIGGKKMVLSGHNFLQDSKVIF
  VEKAPDGHHVWEMEAKTDRDLCKPNSLVLEIPPFRNQRITSPVQVSFYVCNGKRKRSQYQHFTYLPANVP
  IIKTEPTDDFEPALTCGPMSQGLSPLPRPYYSQQLTAPPDPGSCLVAGFAPCSQRNTPMPTSPNASPKLH
  DLSSPAYAKGLANPGHSGHLGLQPPASEAPTMQELPRPMAVQPNSPEQPTSVRLQPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244933
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244933.1, NP_001231862.1
• RefSeq Size: 4132 bp
• RefSeq ORF: 2484 bp
• Locus ID: 100361818
• Cytogenetics: 18q12.3
• MW: 89.2 kDa