Aplp2 (NM_022520) Rat Tagged ORF Clone

CAT#: RR217172

Aplp2 (myc-DDK-tagged) - Rat amyloid beta (A4) precursor-like protein 2 (Aplp2), transcript variant 2

Product Images

Circular map for RR217172

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aplp2
• Synonyms: APLP-2; Apph; WALPLP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217172 representing NM_022520
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCACCGGGACCGCGGCCGCTGCGGCCACCGGCAAGCTTCTTGTCCTGCTGCTGCTCGGGCTCA
  CGGCACCCGCAGCGGCTCTGGCTGGCTATATCGAGGCTCTCGCAGCCAATGCTGGAACAGGCTTTGCGGT
  TGCTGAACCTCAGATTGCCATGTTCTGTGGGAAGCTGAACATGCATGTGAACATTCAGACTGGGAAGTGG
  GAGCCTGACCCAACCGGCACCAAGAGCTGCCTGGGAACAAAGGAGGAAGTTCTTCAGTACTGCCAGGAGA
  TATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCG
  AAGGGACAAAAAACAGTGCAGGAGTCACATTGTTATACCATTCAAGTGTCTTGTGGGTGAATTTGTAAGT
  GATGTTCTGCTAGTTCCAGAAAACTGCCAGTTTTTCCACCAAGAGCGGATGGAGGTGTGTGAGAAGCATC
  AGCGGTGGCACACAGTCGTCAAGGAGGCATGTCTGACTGAGGGGATGACCTTATATAGCTATGGCATGCT
  GCTGCCCTGTGGGGTAGACCAGTTCCATGGCACTGAGTATGTGTGCTGCCCTCAGACAAAGGTTGTTGAC
  TCTGACTCCACTATGTCCAAAGAAGAGGAGGAAGAAGAGGAAGAGGATGAAGAAGAAGACTATGCTCTTG
  ATAAAAGTGAATTTCCTACCGAAGCAGATCTGGAAGACTTCACAGAAGCAGCTGCAGATGAGGACGAGGA
  TGAGGAGGAGGAGGAGGAGGAGGAAGGGGAGGAAGTGGTAGAAGACCGTGATTACTACTATGACTCCTTT
  AAAGGAGATGATTACAATGAGGAGAACCCAACTGAACCCAGCAGCGATGGCACCATCTCAGACAAGGAGA
  TTGCTCACGATGTTAAAGCTGTCTGCTCCCAGGAGGCCATGACGGGGCCCTGCCGGGCCGTGATGCCTCG
  TTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTACGCTTTATATATGGCGGCTGCGGCGGCAACAGGAAC
  AATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAACGATGATTCCCCCGACTCCCCTGCCAACCA
  ATGATGTGGACGTGTATTTTGAGACGTCGGCTGATGACAATGAGCACGCCCGCTTTCAGAAGGCTAAGGA
  GCAGTTGGAAATTCGGCATCGCAGCCGAATGGACAGGGTAAAAAAGGAGTGGGAAGAAGCAGAACTTCAA
  GCCAAGAACCTCCCCAAGGCAGAGCGACAGACCCTCATTCAGCATTTCCAAGCTATGGTTAAAGCCTTAG
  AGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTTGTGGAGACCCATTTGGCCCGAGTAGAAGCAATGCTGAA
  TGACCGTCGGCGCATAGCTCTGGAAAACTACCTGGCTGCCCTGCAGTCTGACCCTCCTCGGCCACATCGC
  ATTCTTCAAGCTCTTCGTCGTTACGTCCGTGCTGAGAACAAAGATCGCCTGCACACCATTCGTCATTACC
  AACATGTGTTGGCTGTTGACCCAGAAAAGGCTGCGCAGATGAAATCCCAGGTGATGACACACCTCCATGT
  CATTGAAGAAAGAAGGAACCAAAGTCTCTCTCTGCTGTACAAAGTGCCTTATGTGGCTCAAGAGATTCAG
  GAGGAAATTGATGAGCTCCTTCAGGAACAGCGAGCAGATATGGACCAGTTTACCTCCTCCATCTCAGAGA
  ACCCTGTGGATGTCCGGGTGAGCTCTGAGGAAAGTGAGGAGATCCCGCCGTTCCACCCTTTCCATCCCTT
  CCCATCCTTGTCTGAGAACGAAGGATCTGGAATGGCAGAGCAAGATGGGGGCCTGATTGGTGCAGAAGAA
  AAGGTGATCAACAGTAAGAATAAAATGGATGAAAATATGGTCATTGACGAGACTCTGGATGTTAAGGAAA
  TGATTTTCAATGCTGAGAGAGTTGGGGGTCTGGAGGAAGAGCCGGACTCTGTGGGGCCTCTACGGGAGGA
  CTTCAGTTTGAGCAGCAGTGCCCTTATTGGCTTGCTGGTCATCGCGGTGGCCATTGCTACAGTCATCGTC
  ATCAGCCTGGTGATGCTGAGGAAGAGGCAATACGGCACCATCAGCCATGGCATTGTGGAGGTTGATCCAA
  TGCTCACCCCAGAAGAGCGTCACTTGAACAAGATGCAGAACCACGGCTATGAAAACCCAACCTACAAATA
  CCTGGAGCAGATGCAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217172 representing NM_022520
  Red=Cloning site Green=Tags(s)
  
  MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKW
  EPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKKQCRSHIVIPFKCLVGEFVS
  DVLLVPENCQFFHQERMEVCEKHQRWHTVVKEACLTEGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKVVD
  SDSTMSKEEEEEEEEDEEEDYALDKSEFPTEADLEDFTEAAADEDEDEEEEEEEEGEEVVEDRDYYYDSF
  KGDDYNEENPTEPSSDGTISDKEIAHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRN
  NFESEDYCMAVCKTMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRSRMDRVKKEWEEAELQ
  AKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHR
  ILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQ
  EEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPFHPFPSLSENEGSGMAEQDGGLIGAEE
  KVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPDSVGPLREDFSLSSSALIGLLVIAVAIATVIV
  ISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022520
• ORF Size: 2259 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022520.1, NP_071965.1
• RefSeq Size: 3608 bp
• RefSeq ORF: 2262 bp
• Locus ID: 64312
• Cytogenetics: 8q13
• MW: 85.4 kDa
• Gene Summary: may contribute to membrane cytoplasmic junctions in sperm membranes [RGD, Feb 2006]