Il12rb1 (NM_001170604) Rat Tagged ORF Clone

CAT#: RR217155

Il12rb1 (myc-DDK-tagged) - Rat interleukin 12 receptor, beta 1 (Il12rb1)

Product Images

Circular map for RR217155

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il12rb1
• Synonyms: Il12rb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217155 representing NM_001170604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATGACGGGGCTGCCAGGGACCAGCAAGCACATCATCTTCCTCCTGCTTTGCCAGCTAAGCGCCT
  CCAGCCGAGGTGACAGCTGCTGCGTTGAGAAGATATCATACCCAGATGAAGCCTCAGGCCCCTCCTTAGG
  ACCCAGGGACTTGAGCTGCTACAGGGTTTCCGGGACAGACTATGAGTGCTCCTGGCAGTATGATGGCCCT
  GAGGACAATGTTGCTCACTTCCTGTGGTGCTGCTTTGCTCATCCAAACCCTACCCACACCAGCTTTGGCC
  AAGAATGCTGCTGCTACTTCTCTTCGGGCCGGTCTCGGACTGTGCAGTTCTGGGAACAGGATGGTATCTC
  TACGTTGTCCAAGGTCAACTTCTGGGTCGAGTCTCGGCTTGGGAACAGGACCTTGAAGTCTCAGGAGATA
  TCGCAGTACCTGTACAACTGGACCAAGCTCACCCCTCCTCTGGGACACATCAGAGTGTCACGATCACGCG
  GGGAGTTGCGAATGGACTGGAATGTGTCGGATGGGGCCGGTGCTGAGGTACAACTGAGGCGCCGGATGCC
  CACAACGAATTGGACCTTGGGTGACTGCGGACCTCAGGTTAGCTCTGGCTTAGGTGTGATTGGTGACATT
  CACGGTAGCAGTTCTGAGTCCTGCCTCTGCCCTTCTGAGAACATGGCCCAGGAAATCCAGATACGGAGAC
  GGCGGCGCCTCTCCTCAGGAGCTCCTGGAGGTCCCTGGAGTAATTGGAGCATGTCTGTGTGTGTCCCGCC
  TGAATTCCTCCCCCAGGCTGAGATTAAGTTCCTGGTGGAGCCCCTGAACCAAGATGGAAGGAGGCGTCTG
  ACTATGGAAGGGCAGACACCACAGCTGGCAGTCCCGGAGGGCTGCAGGGACCGGCCTGGGGCGCAGGTGA
  AGAAGCACTTGGTGCTGGTGCACATGCTGGCCTGCAGGTGCCAGGCTCGGACCTGGAAGACCGTGCCCCT
  GGGCAAGACGCTGGACCTCTCCGGTGCCGCCTACGACCTGGATGTACTCGCCCAGACTCGCTTCGGCCGC
  AGCATCATCCAGAAGTGGCCCCTTCCTGCCCAAGAGCTCACAGAGACAAGAACCCTGAATGTCATCGTGG
  AAGGCAACGTGACATCCATGCAGTGGGCAGCCCAGCCTCCAGGCACTGCCTACTGCCTTGAGTGGCAGCC
  ATGGGGCCAGGACAGGAACCACACGGACTGTACTCTGATTGCACCAGAAGAAGAGGACCCAGCCAAGATG
  GTGACGCACACCTGGAGCTCCAAGCCTACCCTGGACCAGGAGGAATGTTACCGCATCACTGTCTTCGCCT
  CCAAGAACCCCAAGAATCCCATGATGTGGGCCACGGTCCTGTCCAGTTACTACTTTGGGGGTAACGTCTC
  AAGGGTCGGAACTCCACGCCACGTGTCGGTGAGGAACCACACCGAGGACTCAGTGTCCGTGGAGTGGACA
  GCGTCACAGCTGAGCACCTGCCCAGGGGTCCTGACGCAATACGTCGTGCGCTGCGAGGCTGAAGATGGCA
  CGTGGGAGTCAGAGTTGCTCGTGCCACCCACGAAGACCCAAGTGACACTAAACAGTCTGCGCAGCCGCGT
  GGTGTACAAGGTTCAGGTGCGAGCTGACACTGCGATGCTCCCAGGCTCCTGGAGCCGTCCCCAGCGCTTC
  AGCGTTGAGGTGCAGATTTCCCGTTTATCCATCGTTTTCGCGTCTCTGGGAAGCTTCGCCAGCGTCCTCC
  TCGTGGGCAGTCTCGGATACATTGGCTTAAACAGGGCTGCCTGGCACTTGTGCCCACCTCTGCCTACGCC
  CTGTGGCAGCACTGCTGTGGAGTTCCCTGGCAGCCAGGACAAGCAGGCTTGGCAATGGCGCAACCCTGAG
  GACTTCCCCGAGGTGTTGTACCCACGAGACGCGCTGGTGGTGGAGATGCCCAGGGACAGAGATGATGAGA
  CAGAGTCGCCCCAGGCCACCCCTGAGCGCGCCCTGGACACAAGGTGGCCCTTGGGAACTGAGGGGCAGGT
  GCAAGGACTCTCAGAGGCCAGGCTCCCGGACCTGGCTAGAGAGGACTGTCCCCGTGGTGACCTGGCCCAC
  GTGACACTCCCGCTGCTCCTGGGAGATGTGACCCAGGGAGCCTCGGTACTTGGTGATCTTTGGTGGATCC
  ATAAGACCGTGGAGCCAGGACCACCCACTTTGGGTCAAGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217155 representing NM_001170604
  Red=Cloning site Green=Tags(s)
  
  MDMTGLPGTSKHIIFLLLCQLSASSRGDSCCVEKISYPDEASGPSLGPRDLSCYRVSGTDYECSWQYDGP
  EDNVAHFLWCCFAHPNPTHTSFGQECCCYFSSGRSRTVQFWEQDGISTLSKVNFWVESRLGNRTLKSQEI
  SQYLYNWTKLTPPLGHIRVSRSRGELRMDWNVSDGAGAEVQLRRRMPTTNWTLGDCGPQVSSGLGVIGDI
  HGSSSESCLCPSENMAQEIQIRRRRRLSSGAPGGPWSNWSMSVCVPPEFLPQAEIKFLVEPLNQDGRRRL
  TMEGQTPQLAVPEGCRDRPGAQVKKHLVLVHMLACRCQARTWKTVPLGKTLDLSGAAYDLDVLAQTRFGR
  SIIQKWPLPAQELTETRTLNVIVEGNVTSMQWAAQPPGTAYCLEWQPWGQDRNHTDCTLIAPEEEDPAKM
  VTHTWSSKPTLDQEECYRITVFASKNPKNPMMWATVLSSYYFGGNVSRVGTPRHVSVRNHTEDSVSVEWT
  ASQLSTCPGVLTQYVVRCEAEDGTWESELLVPPTKTQVTLNSLRSRVVYKVQVRADTAMLPGSWSRPQRF
  SVEVQISRLSIVFASLGSFASVLLVGSLGYIGLNRAAWHLCPPLPTPCGSTAVEFPGSQDKQAWQWRNPE
  DFPEVLYPRDALVVEMPRDRDDETESPQATPERALDTRWPLGTEGQVQGLSEARLPDLAREDCPRGDLAH
  VTLPLLLGDVTQGASVLGDLWWIHKTVEPGPPTLGQEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170604
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170604.1, NP_001164075.1
• RefSeq Size: 2217 bp
• RefSeq ORF: 2217 bp
• Locus ID: 171333
• Cytogenetics: 16p14
• MW: 82.1 kDa
• Gene Summary: transmembrane protein that belongs to the hemopoietin receptor superfamily; human homolg binds interleukin (IL)12 with a low affinity and may be a part of the IL12 receptor complex [RGD, Feb 2006]