Tgfbi (NM_053802) Rat Tagged ORF Clone

CAT#: RR217102

Tgfbi (myc-DDK-tagged) - Rat transforming growth factor, beta induced (Tgfbi)

Product Images

Circular map for RR217102

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tgfbi
• Synonyms: Big-h3; BIGH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217102 representing NM_053802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCCTCGTGCGGCTGCTGCCCCTCGCTCTGGCTCTGTCTCTGGGCTTCACCGGGACCCTGGCGG
  GCCCGGCCAAGTCACCCTATCAGCTGGTGCTGCAGCACAGCCGGCTCCGGGGCCGCCAGCACGGCCCCAA
  TGTATGTGCTGTGCAGAAGGTCATTGGCACTAACAAGAAATACTTCACCAACTGCAAGCAGTGGTACCAG
  AGGAAGATCTGCGGCAAGTCGACAGTCATCAGCTATGAGTGCTGCCCCGGATATGAAAAGGTCCCAGGAG
  AGAAAGGCTGCCCTGCAGCTCTTCCGCTCTCCAATCTGTACGAGACCTTGGGAATTGTGGGATCGACCAC
  CACACAGCTGTACACAGATCGCACAGAAAAGCTGAGGCCTGAGATGGAGGGACCCGGCAGCTTCACCATC
  TTTGCCCCTAGCAACGAGGCCTGGTCCTCCTTGCCTGCGGAAGTGCTGGACTCCCTGGTGAGCAATGTCA
  ACATTGAACTGCTCAACGCCCTCCGCTACCACATGGTGGACAGACGGGTCCTGACAGACGAGCTTAAGCA
  CGGCATGGCTCTCACCTCCATGTACCAGAACTCCAACATCCAGATCCATCACTATCCCAATGGGATTGTA
  ACTGTTAACTGTGCCCGGCTGCTGAAAGCTGACCACCATGCGACCAACGGCGTGGTGCATCTCATTGATA
  AGGTCATTTCCACCGTTACCAACAACATCCAGCAGATCATCGAGATTGAGGACACCTTTGAGACACTTCG
  GGCCGCCGTGGCTGCATCAGGACTCAATACCGTGCTGGAGGGTGACGGCCAGTTCACACTCTTGGCCCCA
  ACCAACGAGGCCTTTGAGAAGATTCCTGCTGAGACCTTGAACCGGATCCTGGGTGACCCCGAGGCCCTGA
  GAGACCTGCTAAACAATCATATCCTGAAGACAGCCATGTGTGCTGAGGCCATTGTGGCTGGAATGGCCAT
  GGAGACCCTGGGGGGCACCACACTGGAGGTGGGCTGCAGTGGAGACATGCTCACTATTAACGGGAAGGCT
  GTCATCTCCAACAAAGACATCCTGGCCACCAACGGTGTCATCCACTTCATTGATGAGCTGCTCATCCCAG
  ATTCAGCCAAGACATTGCTCGAGCTCGCTTCGGAATCTGACGTCTCCACAGCCATTGACCTCCTCAGACA
  AGCTGGCCTCAGCACGCATCTCTCTGGAAAAGAACAGTTGACCTTCCTGGCCCCTCTGAATTCTGTGTTC
  AAAGATGGTGTCCCCCGCATTGACGGCCAAATGAAGACTTTGCTTCTGAACCACATGGTCAAAGGGCAGT
  TGGCCTCCAAATATTTGTACTCCGGACAGACACTGGACACACTGGGTGGCAAAAAGCTTCGAGTTTTTGT
  TTATCGAAATAGCCTCTGCATTGAAAACAGCTGCATTGCCGCCCATGACAAGAAGGGACGGTATGGGACC
  CTGTTCACCATGGACCGGATGCTGACACCCCCTATGGGGACTGTTATGGATGTCCTGAAGGGGGACAACC
  GTTTTAGCATGCTGGTGGCCGCCATCCAGTCTGCAGGCCTGATGGAGACCCTCAACCGGGAAGGGGTCTA
  CACTGTTTTCGCTCCCACGAACGAAGCGTTCCAAGCCATGCCTCCAGAAGAGCTGAACAAACTCTTGGCA
  AATGCCAAGGAACTTACCAACATCCTGAAGTACCACATTGGGGATGAGATCCTGGTTAGCGGAGGCATTG
  GGGCTCTGGTGCGGCTGAAGTCTCTCCAAGGGGACAAACTGGAAGTCAGCTCGAAAAACAATGTGGTGAG
  TGTCAATAAGGAACCTGTTGCCGAAACCGACATCATGGCCACAAACGGTGTGGTGTATGCCATCAACACT
  GTTCTGCAACCGCCAGCCAACCGGCCACAAGAACGGGGAGAAGAGTTGGCAGACTCTGCCCTTGAAATCT
  TCAAGAAGGCGTCAGCCTATTCCAGGAGTGCCCAGAGTTCTATGCGACTTGCCCCTGTCTATCAGCGGTT
  ACTAGAGAGGATGAAGCGGAGAGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217102 representing NM_053802
  Red=Cloning site Green=Tags(s)
  
  MALLVRLLPLALALSLGFTGTLAGPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNKKYFTNCKQWYQ
  RKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGIVGSTTTQLYTDRTEKLRPEMEGPGSFTI
  FAPSNEAWSSLPAEVLDSLVSNVNIELLNALRYHMVDRRVLTDELKHGMALTSMYQNSNIQIHHYPNGIV
  TVNCARLLKADHHATNGVVHLIDKVISTVTNNIQQIIEIEDTFETLRAAVAASGLNTVLEGDGQFTLLAP
  TNEAFEKIPAETLNRILGDPEALRDLLNNHILKTAMCAEAIVAGMAMETLGGTTLEVGCSGDMLTINGKA
  VISNKDILATNGVIHFIDELLIPDSAKTLLELASESDVSTAIDLLRQAGLSTHLSGKEQLTFLAPLNSVF
  KDGVPRIDGQMKTLLLNHMVKGQLASKYLYSGQTLDTLGGKKLRVFVYRNSLCIENSCIAAHDKKGRYGT
  LFTMDRMLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLMETLNREGVYTVFAPTNEAFQAMPPEELNKLLA
  NAKELTNILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSSKNNVVSVNKEPVAETDIMATNGVVYAINT
  VLQPPANRPQERGEELADSALEIFKKASAYSRSAQSSMRLAPVYQRLLERMKRRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053802
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053802.1, NP_446254.1
• RefSeq Size: 2696 bp
• RefSeq ORF: 2058 bp
• Locus ID: 116487
• Cytogenetics: 17p14
• MW: 74.8 kDa
• Gene Summary: mouse homolog is an extracellular matrix protein; human homolog is associated with hereditary corneal dystrophies [RGD, Feb 2006]