Mx1 (NM_001271059) Rat Tagged ORF Clone

CAT#: RR217052

Mx1 (myc-DDK-tagged) - Rat myxovirus (influenza virus) resistance 1 (Mx1), transcript variant 3

Product Images

Circular map for RR217052

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mx1
• Synonyms: IFI78
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217052 representing NM_001271059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCAGACCTCTGCATGCCGTCACCAGACACCACAGAAACATCCTGATACCTCTGAAGAGAGCC
  AGGCAATGGAGTCTGTGGATAACCTCTGCAGTCAGTATGAGGAGAAGGTGCGGCCCTGCATTGACCTCAT
  CGACTCCCTGCGAGCTCTGGGTGTGGAGCAAGACCTGGCCCTGCCTGCCATCGCTGTCATCGGGGACCAG
  AGTTCAGGGAAGAGCTCTGTGCTGGAAGCACTGTCTGGAGTGGCCCTCCCCAGAGGCAGTGGTATTGTTA
  CCAGGTGTCCTCTGGTGCTGAAATTGAAGCAACTGAAGCAGGGAGAAAAATGGAGCGGCAAGGTCATTTA
  TAAGGACACCGAGATTGAGATCTCACACCCTTCACTGGTAGAAAGGGAAATCAATAAAGCCCAGAACTTG
  ATTGCTGGGGAAGGGTTGAAGATTAGCTCTGATCTCATTAGCTTGGAGGTTAGCTCTCCACATGTCCCAG
  ACCTGACTCTGATTGACCTTCCTGGTATCACAAGAGTGGCTGTGGGTGACCAGCCTGCAGACATCGAACA
  CAAGATCAAGAGACTTATCACTGAATACATCCAGAAACAGGAGACCATCAACCTGGTGGTGGTCCCCAGC
  AATGTGGACATTGCCACCACAGAGGCCCTGAAAATGGCTCAGGAGGTGGACCCTCAAGGGGATAGAACCA
  TAGGGATCTTGACCAAGCCCGATCTGGTGGACAGAGGGACTGAAGACAAGGTCGTGGATGTGGTGCGGAA
  CCTCGTGTGCCACCTGAAGAAGGGCTACATGATAGTCAAGTGCAGGGGTCAGCAGGACATCCAGGAGCAG
  CTGAGCCTGGCTGAGGCTCTTCAGAAGGAGCAAGTCTTCTTCAAGGAACACCCTCAATTCAGAGTTCTTC
  TCGAGGATGGGAAGGCCACAGTGCCCTGCTTGGCAAAGAGACTGACCATGGAGCTCACCTCCCACATCTG
  TAAATCGCTGCCAATATTGGAAAATCAAATAAATGTGAATCATCAGATTGCGAGTGAGGAGCTGCAGAAG
  TACGGAGCAGACATACCAGAAGATGACAGTAAGAGGCTGTCCTTTCTGATGAATAAAATCAATGTCTTCA
  ATAAGGATATCCTAAGTTTGGTACAAGCACAAGAAAACATATCATGGGAAGAAAGCCGGCTGTTCACCAA
  ACTGCGAAATGAGTTTCTTGCTTGGAATGATTATATTGAGGAACATTTCAAAAAAACCCTAGGTTCTTCT
  GAGAAACACAGCCAGATGGAAAAATTTGAAAGTCATTATCGTGGCCGGGAACTGCCAGGGTTTGTGGACT
  ATAAGGCATTTGAGAACATTATCAAGAAGGAAGTCAAGGCCCTGGAAGAACCCGCTCTGAACATGCTGCA
  CAGGGTCACTACCATGGTCAAAAATGCCTTCACAAAGGTTTCATCAAACAATTTTGGTGATTTTTTAAAC
  CTCCACAGTACTGCCAAGTCCAAAATTGAAGACATCAGATTTAGCCAAGAAAAAGAAGCCGAAAAACTGA
  TCCGACTTCACTTCCAGATGGAACACATTGTCTACTGCCAGGACCAGGCTTACAAGAAAGCCTTGAAGGA
  GATCAGAGAGAAGGAAGCTGAGAAAGAGAAGTCAACCTTTGGTGTCTTTCAACAAAACTCTCCTCGAAAG
  GAGTTGACTACCACTGAGATGACCCAGCACCTGAATGCCTACTACCAGGAGTGCGGAAGGAACATTGGGA
  GACAGATCCCTCTGATCATCCAGTACTCCATCCTGCAAACATTTGGGCAGGAAATGGAGAAAGCAATGCT
  TCAGCTTCTACAGGACACCAGTAAGTGCAACTGGTTCCTGACTGAGCAGAGTGACAGCAGAGAGAAGAAG
  AAGTTCCTGAAGAGGCGGCTTTTAAGGCTGGATGAGGCTCAGCGGAAGCTTGCCAAATTCTCCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217052 representing NM_001271059
  Red=Cloning site Green=Tags(s)
  
  MKEQTSACRHQTPQKHPDTSEESQAMESVDNLCSQYEEKVRPCIDLIDSLRALGVEQDLALPAIAVIGDQ
  SSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKQLKQGEKWSGKVIYKDTEIEISHPSLVEREINKAQNL
  IAGEGLKISSDLISLEVSSPHVPDLTLIDLPGITRVAVGDQPADIEHKIKRLITEYIQKQETINLVVVPS
  NVDIATTEALKMAQEVDPQGDRTIGILTKPDLVDRGTEDKVVDVVRNLVCHLKKGYMIVKCRGQQDIQEQ
  LSLAEALQKEQVFFKEHPQFRVLLEDGKATVPCLAKRLTMELTSHICKSLPILENQINVNHQIASEELQK
  YGADIPEDDSKRLSFLMNKINVFNKDILSLVQAQENISWEESRLFTKLRNEFLAWNDYIEEHFKKTLGSS
  EKHSQMEKFESHYRGRELPGFVDYKAFENIIKKEVKALEEPALNMLHRVTTMVKNAFTKVSSNNFGDFLN
  LHSTAKSKIEDIRFSQEKEAEKLIRLHFQMEHIVYCQDQAYKKALKEIREKEAEKEKSTFGVFQQNSPRK
  ELTTTEMTQHLNAYYQECGRNIGRQIPLIIQYSILQTFGQEMEKAMLQLLQDTSKCNWFLTEQSDSREKK
  KFLKRRLLRLDEAQRKLAKFSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271059
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271059.1, NP_001257988.1
• RefSeq Size: 3247 bp
• RefSeq ORF: 1959 bp
• Locus ID: 24575
• Cytogenetics: 11q12
• MW: 74.5 kDa
• Gene Summary: mouse homolog is an interferon inducible gene that confers influenza resistance to mice [RGD, Feb 2006]