Foxo1 (NM_001191846) Rat Tagged ORF Clone

CAT#: RR217044

Foxo1 (myc-DDK-tagged) - Rat forkhead box O1 (Foxo1)

Product Images

Circular map for RR217044

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxo1
• Synonyms: Fkhr; Foxo1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217044 representing NM_001191846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAGCGCCTCAGGTGGTGGAGACCGACCCGGACTTCGAGCCGCTGCCTCGGCAGCGCTCCTGCA
  CCTGGCCGCTGCCCAGGCCGGAGTTTAACCAGTCCAACTCGACCACCTCCAGCCCGGCGCCGTCGGGCAG
  CACGGCCGCCAACCCCGACGCCACGGCGAGCCTGGCCTCGGCGTCCGCTGTCAGCACCGACTTTATGAGC
  AACCTGAGCCTGCTAGAAGAGAGCGAGGACTTCGCGCGGGCACCGGGTTGCGTGGCCGTGGCGGCCGCAG
  CTGCCGCCAGTAGGGGCCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTGCGTGCACTCGGCGCCGCC
  GCAGCCCCCACCGACCGGGCCGCTGTCGCAGCCCCCACCGGTGCCTCCAGCCGCCGCGGGGCCACTCGCT
  GGGCAGCCGCGCAAGACCAGTTCGTCGCGCCGCAACGCGTGGGGCAACCTGTCGTACGCCGACCTCATCA
  CCAAGGCCATCGAGAGCTCGGCGGAGAAGAGGCTCACCCTGTCGCAGATCTACGAGTGGATGGTGAAGAG
  TGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCGGCGGGCTGGAAGAATTCAATTCGCCACAAT
  CTGTCCCTACACAGCAAGTTTATTCGAGTACAGAATGAAGGAACTGGGAAAAGTTCTTGGTGGATGCTCA
  ATCCAGAGGGAGGCAAGAGTGGGAAATCACCCCGGAGAAGAGCTGCATCCATGGACAACAACAGTAAATT
  TGCTAAGAGCCGAGGACGGGCTGCTAAGAAAAAAGCATCTCTCCAGTCCGGGCAAGAGGGTCCTGGAGAT
  AGCCCTGGGTCTCAGTTTTCTAAATGGCCTGCAAGCCCTGGGTCCCACAGCAATGATGACTTTGATAACT
  GGAGTACATTTCGTCCTCGAACCAGCTCAAACGCTAGCACCATCAGTGGGAGACTTTCTCCCATCATGAC
  AGAGCAGGATGACCTGGGGGATGGGGATGTGCATTCCCTGGTGTATCCACCCTCTGCTGCCAAGATGGCG
  TCTACACTGCCCAGTCTGTCTGAAATCAGCAATCCAGAAAACATGGAGAACCTTCTGGATAATCTCAATC
  TTCTCTCATCCCCAACATCTTTAACTGTGTCAACCCAGTCCTCGCCTGGCAGCATGATGCAGCAGACACC
  TTGCTATTCATTTGCACCGCCAAACACCAGTCTAAATTCCCCCAGCCCCAACTACGCAAAGTACACGTAC
  GGCCAATCCAGCATGAGCCCTGTGCCCCAGATGCCTATGCAGACACTTCAGGACAGCAAATCAAGTTATG
  GAGGATTGAACCAGTATAACTGTGCCCCAGGACTCTTGAAAGAGTTGCTGACTTCTGACTCTCCTCCCCA
  CAATGATATTATGTCACCAGTCGATCCTGGAGTGGCTCAACCCAACAGTCGGGTCCTGGGCCAAAATGTA
  CTGATGGGCCCTAACTCAGTCATGCCAGCCTATGGCAGCCAGGCACCTCATAACAAAATGATGAACCCCA
  GTTCCCACACCCACCCTGGACATGCACAGCAAACTTCTTCAGTCAATGGCCGTGCCCTGCCCCATGTGGT
  GAACACCATGCCTCACACATCTGCCATGAACCGCTTGACCCCAGTGAAGACACCTTTACAAGTGCCTCTG
  TCCCACCCCATGCAGATGAGTGCCCTGGGCAACTACTCCTCTGTGAGCAGCTGCAATGGCTATGGTAGGA
  TGGGTGTCCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGGCATGTTTATTGAGCGCTTGGACTGTGA
  CATGGAGTCCATCATTCGGAACGACCTCATGGACGGAGATACCTTGGATTTTAACTTTGATAATGTGTTG
  CCCAACCAAAGCTTCCCGCACAGTGTCAAGACTACAACACACAGCTGGGTGTCAGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217044 representing NM_001191846
  Red=Cloning site Green=Tags(s)
  
  MAEAPQVVETDPDFEPLPRQRSCTWPLPRPEFNQSNSTTSSPAPSGSTAANPDATASLASASAVSTDFMS
  NLSLLEESEDFARAPGCVAVAAAAAASRGLCGDFQGPEAGCVHSAPPQPPPTGPLSQPPPVPPAAAGPLA
  GQPRKTSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHN
  LSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGPGD
  SPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSLVYPPSAAKMA
  STLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGSMMQQTPCYSFAPPNTSLNSPSPNYAKYTY
  GQSSMSPVPQMPMQTLQDSKSSYGGLNQYNCAPGLLKELLTSDSPPHNDIMSPVDPGVAQPNSRVLGQNV
  LMGPNSVMPAYGSQAPHNKMMNPSSHTHPGHAQQTSSVNGRALPHVVNTMPHTSAMNRLTPVKTPLQVPL
  SHPMQMSALGNYSSVSSCNGYGRMGVLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVL
  PNQSFPHSVKTTTHSWVSG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191846
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191846.2, NP_001178775.1
• RefSeq Size: 2560 bp
• RefSeq ORF: 1950 bp
• Locus ID: 84482
• UniProt ID: G3V7R4
• Cytogenetics: 2q26
• MW: 69.3 kDa
• Gene Summary: mediates effects of insulin on glucose-6-phosphatase gene expression [RGD, Feb 2006]