Gcgr (NM_172091) Rat Tagged ORF Clone

CAT#: RR216771

Gcgr (myc-DDK-tagged) - Rat glucagon receptor (Gcgr), transcript variant 2

Product Images

Circular map for RR216771

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gcgr
• Synonyms: MGC93090
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216771 representing NM_172091
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTCACCCAGCTCCACTGTCCCTACCTGCTGCTGCTGCTGGTGGTGCTGTCATGTCTGCCAAAGG
  CACCCTCTGCCCAGGTAATGGACTTTTTGTTTGAGAAGTGGAAGCTCTATAGTGACCAGTGCCACCACAA
  CCTAAGCCTGCTGCCCCCACCTACTGAGCTGGTCTGCAACAGAACTTTCGACAAGTACTCCTGCTGGCCT
  GACACCCCTCCCAACACCACTGCCAACATTTCCTGCCCCTGGTACCTACCTTGGTACCACAAAGTGCAGC
  ACCGCCTAGTGTTCAAGAGGTGTGGGCCTGATGGGCAGTGGGTTCGAGGGCCACGGGGGCAGTCATGGCG
  CGACGCCTCCCAATGTCAGATGGATGATGACGAGATCGAGGTCCAGAAGGGGGTAGCCAAGATGTATAGC
  AGCTACCAGGTGATGTACACTGTGGGCTACAGTCTGTCCCTGGGGGCCTTGCTCCTGGCGCTGGTCATCC
  TGCTGGGCCTCAGGAAGCTGCACTGCACCCGGAACTACATCCACGGGAACCTGTTCGCGTCCTTCGTGCT
  CAAGGCTGGCTCTGTGCTGGTCATTGATTGGCTGCTCAAGACACGCTATAGCCAGAAGATTGGAGATGAC
  CTCAGTGTGAGCGTCTGGCTCAGTGATGGGGCGGTGGCTGGCTGCAGAGTGGCCACAGTGATCATGCAGT
  ACGGCATCATAGCCAACTACTGCTGGTTGCTGGTGGAGGGTGTGTACCTGTACAGCCTGCTGAGCATCAC
  CACCTTCTCGGAGAAGAGCTTCTTCTCCCTCTATCTGTGCATCGGCTGGGGATCTCCCCTGCTGTTTGTC
  ATCCCCTGGGTGGTGGTCAAGTGTCTGTTTGAGAATGTCCAGTGCTGGACCAGCAATGACAATATGGGAT
  TCTGGTGGATCCTGCGTATCCCTGTACTCCTGGCCATACTGATCAATTTTTTCATCTTTGTCCGCATCAT
  TCATCTTCTTGTGGCCAAGCTGCGTGCCCATCAGATGCACTATGCTGATTACAAGTTCCGGCTAGCCAGG
  TCCACGCTGACCCTCATTCCTCTGCTGGGAGTCCACGAAGTGGTCTTTGCCTTTGTGACTGATGAGCATG
  CCCAGGGCACCCTGCGCTCCACCAAGCTCTTTTTTGACCTGTTCTTCAGCTCCTTCCAGGGTCTGCTGGT
  GGCTGTTCTCTACTGTTTCCTCAACAAGGAGGTGCAGGCAGAGCTACTGCGGCGTTGGAGGCGATGGCAA
  GAAGGCAAAGCTCTTCAGGAGGAAAGGATGGCCAGCAGCCATGGCAGCCACATGGCCCCAGCAGGGACTT
  GTCATGGTGATCCCTGTGAGAAACTTCAGCTTATGAGTGCAGGCAGCAGCAGTGGGACTGGCTGTGAGCC
  CTCTGCGAAGACCTCATTGGCCAGTAGTCTCCCAAGGCTGGCTGACAGCCCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216771 representing NM_172091
  Red=Cloning site Green=Tags(s)
  
  MLLTQLHCPYLLLLLVVLSCLPKAPSAQVMDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWP
  DTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYS
  SYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDD
  LSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFV
  IPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLAR
  STLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQ
  EGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172091
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172091.2, NP_742088.1
• RefSeq Size: 1893 bp
• RefSeq ORF: 1458 bp
• Locus ID: 24953
• UniProt ID: P30082
• Cytogenetics: 10q32.3
• MW: 55 kDa
• Gene Summary: G-protein coupled receptor that binds glucagon and activates adenylate cyclase [RGD, Feb 2006]