Hnf4a (NM_001270931) Rat Tagged ORF Clone

CAT#: RR216701

Hnf4a (myc-DDK-tagged) - Rat hepatocyte nuclear factor 4, alpha (Hnf4a), transcript variant 2

Product Images

Circular map for RR216701

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnf4a
• Synonyms: Hnf4; Hnf4alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216701 representing NM_001270931
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACAGGGGCCAGGGTTTCCTGCAGGACCCACCAGACACATCCCCATCTGAAGGTGCCAACCTCA
  ACTCATCCAACAGCCTGGGTGTCAGTGCCCTGTGTGCCATCTGTGGCGATCGGGCCACTGGCAAACACTA
  CGGAGCCTCAAGCTGTGACGGCTGCAAGGGATTCTTCAGGAGGAGCGTGAGGAAGAACCACATGTACTCC
  TGCAGGTTTAGCAGGCAGTGCGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCTCAAGA
  AGTGCTTCCGGGCTGGCATGAAGAAAGAAGCCGTCCAAAATGAGCGGGATCGGATCAGCACGCGGAGGTC
  AAGCTACGAGGACAGCAGCCTACCCTCCATTAATGCGCTCCTGCAGGCAGAGGTCCTGTCTCAGCAGATC
  ACCTCCCCCATCTCTGGGATCAATGGCGACATTCGGGCCAAGAGGATTGCCAACATCACGGATGTGTGTG
  AGTCTATGAAGGAGCAGCTGCTGGTTCTGGTCGAATGGGCCAAGTACATCCCGGCCTTCTGTGAACTTCT
  TCTGGATGACCAGGTGGCGCTGCTCAGAGCCCACGCTGGTGAGCACCTGCTGCTTGGAGCCACCAAGAGG
  TCCATGGTGTTCAAGGATGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGAGCTAG
  CAGAGATGAGCCGTGTGTCCATTCGCATCCTCGATGAGCTGGTCTTGCCCTTCCAAGAGCTGCAGATCGA
  TGATAATGAATACGCCTGCCTCAAAGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGTGACCCA
  GGCAAGATCAAGCGGCTGCGGTCACAGGTGCAGGTGAGCCTGGAGGATTACATCAACGACCGGCAGTATG
  ACTCTCGGGGTCGTTTTGGAGAGCTGCTGCTGCTCCTGCCCACTCTGCAGAGCATTACCTGGCAGATGAT
  CGAGCAGATCCAGTTCATCAAGCTCTTTGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGCTGCTT
  GGAGGGTCTGCCAGTGACGCGCCCCACGCCCACCACCCCCTGCACCCTCACCTGATGCAAGAACACATGG
  GCACCAATGTCATAGTTGCCAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTGTGAGTGGCCCCG
  GCCCAGGGGGCAGGCAGCCACCCCTGAGACTCCACAGCCATCACCACCAAGTGGCTCTGGATCTGAATCC
  TACAAGCTCCTGCCAGGAGCCATCACCACCATCGTCAAGCCTCCCTCTGCCATCCCCCAGCCAACGATCA
  CCAAGCAGGAAGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216701 representing NM_001270931
  Red=Cloning site Green=Tags(s)
  
  MSDRGQGFLQDPPDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYS
  CRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQI
  TSPISGINGDIRAKRIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKR
  SMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDP
  GKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLL
  GGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSES
  YKLLPGAITTIVKPPSAIPQPTITKQEAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270931
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270931.1, NP_001257860.1
• RefSeq Size: 4456 bp
• RefSeq ORF: 1350 bp
• Locus ID: 25735
• Cytogenetics: 3q42
• MW: 50 kDa
• Gene Summary: steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006]