Crhr1 (NM_001301812) Rat Tagged ORF Clone

CAT#: RR216605

Crhr1 (myc-DDK-tagged) - Rat corticotropin releasing hormone receptor 1 (Crhr1), transcript variant 2

Product Images

Circular map for RR216605

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr1
• Synonyms: CRFR1; CRH-R 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216605 representing NM_001301812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCTGTGTCCACCT
  CCCTTCAGGATCAGCGCTGTGAGAACCTGTCCCTGACCAGCAATGTTTCTGGCCTGCAGTGCAATGCATC
  CGTGGACCTCATTGGCACCTGCTGGCCCCGGAGCCCTGCGGGCCAGTTGGTGGTCCGACCCTGCCCTGCC
  TTTTTCTACGGTGTCCGCTACAACACGACAAACAATGGCTACCGGGAGTGCCTGGCCAACGGCAGCTGGG
  CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAGCAAAGTACACTACCATGT
  TGCAGTCATCATCAACTACCTGGGTCACTGCATCTCCCTGGTAGCCCTCCTGGTGGCCTTTGTCCTCTTC
  TTGCGGCTCAGGAGCATCCGGTGCCTGAGAAACATCATCCACTGGAACCTCATCTCGGCTTTCATCCTAC
  GCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTGCACCAGAGCAATGTGGCCTGGTG
  TAGGTTGGTGACAGCCGCCTACAATTACTTCCATGTAACCAACTTCTTCTGGATGTTCGGTGAGGGCTGC
  TACCTGCACACAGCCATTGTGCTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCATTG
  GCTGGGGTGTACCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAGCTGCACTACGACAATGAAAAGTG
  CTGGTTTGGCAAACGTCCTGGGGTATACACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTGCTG
  ATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGGGCATCCACTACATCTG
  AGACCATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCCTGCCCCTTCTGGGCATCACCTACAT
  GTTGTTCTTCGTCAACCCTGGAGAGGACGAGGTCTCCAGGGTCGTCTTCATCTACTTCAACTCTTTTCTG
  GAGTCCTTTCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTTCTGAACAGTGAGGTCCGCTCCGCTATCC
  GGAAGAGGTGGCGTCGGTGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGAGCTATGTCCATCCC
  CACCTCCCCGACCAGAGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216605 representing NM_001301812
  Red=Cloning site Green=Tags(s)
  
  MGRRPQLRLVKALLLLGLNPVSTSLQDQRCENLSLTSNVSGLQCNASVDLIGTCWPRSPAGQLVVRPCPA
  FFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKSKVHYHVAVIINYLGHCISLVALLVAFVLF
  LRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTVSPEVHQSNVAWCRLVTAAYNYFHVTNFFWMFGEGC
  YLHTAIVLTYSTDRLRKWMFVCIGWGVPFPIIVAWAIGKLHYDNEKCWFGKRPGVYTDYIYQGPMILVLL
  INFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFL
  ESFQGFFVSVFYCFLNSEVRSAIRKRWRRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301812
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301812.1, NP_001288741.1
• RefSeq Size: 2507 bp
• RefSeq ORF: 1245 bp
• Locus ID: 58959
• Cytogenetics: 10q32.1
• MW: 47.7 kDa
• Gene Summary: This gene encodes a member of the G-protein coupled receptor family. The encoded protein is a receptor for corticotropin-releasing factor (CRH) and urocortin (UCN). The interaction of this protein with CRH and UCN triggers G-protein coupled signaling. This protein plays a pivotal role in mediating the CRH-elicited effects in depression and anxiety. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]