Rnh1 (NM_139105) Rat Tagged ORF Clone

CAT#: RR215630

LOC100360501 (Myc-DDK-tagged ORF) - Rat ribonuclease/angiogenin inhibitor 1 (Rnh1), (10 ug)

Product Images

Circular map for RR215630

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnh1
• Synonyms: Rnh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215630 representing NM_139105
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTTGACATCCAGTGTGAGCAGCTGAGTGATGCCCGGTGGACAGAGCTCCTTCCCCTGATCCAAC
  AATACCAAGTGGTCAGGCTGGATGACTGTGGCCTCACTGAAGTGCGGTGCAAAGACATCAGGTCAGCGAT
  CCAGGCCAACCCTGCCCTGACAGAGCTCAGCCTACGCACCAATGAACTGGGTGATGCTGGTGTGGGTCTG
  GTGCTCCAGGGCCTGCAGAATCCCACTTGTAAGATCCAGAAGCTGAGCCTTCAGAACTGCAGCTTGACGG
  AAGCTGGCTGTGGGGTCCTGCCTGATGTGCTGCGCTCTTTGTCTACCCTGCGTGAACTACATCTCAATGA
  CAACCCTCTGGGGGATGAAGGCCTGAAGCTGCTCTGTGAAGGACTCCGGGACCCCCAGTGCCGTCTTGAG
  AAGCTTCAGTTGGAATACTGTAACCTCACAGCTACCAGCTGCGAGCCCCTGGCCTCAGTGCTCAGGGTGA
  AACCTGACTTTAAAGAGCTAGTATTGAGCAACAATGACTTCCATGAGGCTGGTATCCACACTCTGTGCCA
  GGGCCTGAAGGATTCTGCCTGTCAACTGGAGTCACTCAAACTGGAGAACTGTGGTATCACATCAGCCAAC
  TGCAAGGATCTGTGTGATGTTGTGGCCTCCAAAGCCTCACTGCAAGAACTGGACTTGGGCAGCAACAAGC
  TGGGCAACACAGGCATTGCAGCACTGTGCTCAGGACTGCTGCTTCCCAGCTGCAGGCTGAGGACTCTGTG
  GCTCTGGGACTGTGATGTCACTGCAGAAGGCTGCAAGGACCTGTGCCGTGTCCTCAGAGCCAAGCAGAGC
  CTGAAGGAACTCAGCCTAGCTGGCAATGAGCTGAAGGATGAGGGTGCCCAACTGCTGTGTGAGAGCCTGT
  TAGAGCCTGGCTGTCAGCTGGAGTCACTGTGGGTAAAGACCTGTAGCCTCACAGCTGCCTCTTGTCCCCA
  CTTCTGCTCGGTGTTGACCAAAAATCGTTCTCTGTTTGAGCTGCAAATGAGCAGCAACCCGCTGGGAGAC
  TCGGGAGTCGTGGAGCTTTGCAAGGCCCTGGGCTATCCGGACACAGTGCTGCGTGTGCTTTGGCTGGGAG
  ACTGTGATGTGACAGACAGTGGCTGCAGCAGCCTTGCCACTGTCCTGCTGGCCAACCGCAGCTTGAGGGA
  ACTGGACCTCAGTAACAACTGCATGGGGGACACCGGTGTCCTACAACTGCTGGAGAGCCTCAAACAGCCC
  AGCTGCGCCCTTCAGCAGCTTGTCCTGTATGACATTTACTGGACGGATGAGGTGGAAGACCAGCTTCGGG
  CCCTGGAGGAGGAAAGGCCATCCCTGAGGATCATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215630 representing NM_139105
  Red=Cloning site Green=Tags(s)
  
  MSLDIQCEQLSDARWTELLPLIQQYQVVRLDDCGLTEVRCKDIRSAIQANPALTELSLRTNELGDAGVGL
  VLQGLQNPTCKIQKLSLQNCSLTEAGCGVLPDVLRSLSTLRELHLNDNPLGDEGLKLLCEGLRDPQCRLE
  KLQLEYCNLTATSCEPLASVLRVKPDFKELVLSNNDFHEAGIHTLCQGLKDSACQLESLKLENCGITSAN
  CKDLCDVVASKASLQELDLGSNKLGNTGIAALCSGLLLPSCRLRTLWLWDCDVTAEGCKDLCRVLRAKQS
  LKELSLAGNELKDEGAQLLCESLLEPGCQLESLWVKTCSLTAASCPHFCSVLTKNRSLFELQMSSNPLGD
  SGVVELCKALGYPDTVLRVLWLGDCDVTDSGCSSLATVLLANRSLRELDLSNNCMGDTGVLQLLESLKQP
  SCALQQLVLYDIYWTDEVEDQLRALEEERPSLRIIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139105
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139105.3, NP_620805.2
• RefSeq Size: 1688 bp
• RefSeq ORF: 1371 bp
• Locus ID: 100360501
• UniProt ID: P29315
• Cytogenetics: 1q41
• MW: 49.9 kDa
• Gene Summary: ribonuclease inhibitor [RGD, Feb 2006]