Lrrc14 (NM_001024354) Rat Tagged ORF Clone

CAT#: RR215593

Lrrc14 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 14 (Lrrc14), (10 ug)

Product Images

Circular map for RR215593

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc14
• Synonyms: MGC108882
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215593 representing NM_001024354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACACGCTTGTGTTCCTCAGCACACGCCAGGTGCTTCAGTGCCAGCCAGCTGCCTGCCAGGCCTTGC
  CCCTGCTGCCCCGAGAACTCTTCCCTCTGCTGTTCAAGGTGGCCTTCATGGACAAGAAGACAGCCGTGCT
  GCGTGAGCTGGTGCACACGTGGCCCTTTCCCCTCCTCAGTTTCCAGCAGCTGCTGCAGGAGTGTGCCCAC
  TGCAGTCGAGCCCTGCTGCAGGAACGGCTCAGCACAGAAAGCATGCAGGCTGTGATCCTGGGACTGACCG
  CCCGGATACATGCTCGAGAAGCAGAGGCTGGCACACAGCCCGTTTGCAGGAAGCATACACTGCGGGTACT
  GGACATGACTGGCCTCCTGGATGATGGTGTGGAGCAGGATCCAGGAACCATGAGCATGTGGGACTGTACA
  GCGGCGGTAGCCAGAACTTGTATTGCCCAGCAGCAGGTCGGGACTGCTAAGCCAGGGCTGTCACCAGTCC
  CCGTGGAAATTCGTGTAGACCTTCGGGTAAACCGGGCCTCCTATACATTCCTTCGTGAGGCACTCCAGAG
  TAGTGTGGACAGCCTGCTGCGGCTCTGCTGCCGGGACCTCCGGGCTGAGGATCTGCCCATGCGTAACACT
  GTGGCCCTACTACAGCTTCTAGATGCAGGCTGTCTGCGCCGTATAGACCTGCGCTTCAATAACCTGGGCT
  TGCGTGGCCTGTCTGTCATTATCCCACATGTGGCCCGCTTTCAGCACCTAGCCAGCCTGAGGCTGCATTA
  TGTGCATGGGGACTCCAGACAGCCCTCCGTGGATGGTGAGGACAACTTTCGCTACTTCCTGGCCCAGATG
  GGCCGGTTCATCTGTCTTCGGGAGCTCAGCATTGGCTCTTCTCTCCTCTCAGGCAGGCTGGATCAACTGC
  TCAGCACCCTGCAGAGGCCCCTGGAAAGCCTAGAGCTGGCCTTCTGTGCACTGTTGCCTGAGGACCTACG
  CTTCCTGGCTCAGAGCTCTCATGCTGCCCACCTCAAAAAGCTGGACTTGAGTGGCAATGACTTGTCAGGC
  AACCAGCTGACACCCTTCCAGGGTCTGCTGCAGGCAGTAGCAGCCACACTGCTGCATCTTGAGCTGACTG
  AGTGCCAGATTGCTGATGCCCAGTTGTTGGCCACACTGCCCACCCTGACTCGCTGTGCCAGTCTCCGATA
  CCTTGGTCTCTATGGCAACCCTTTGTCTATGGCAGGACTTAAAGAGCTGCTTCGGGACTCTGTGATTCAG
  GCTGAGCTACGTACTGTGGTGCACCCTTTCCCTGTGGACTGCTATGAGGGTCTTCCTTGGCCGCCACCTG
  CCTCTGTCCTTCTGGAAGCCTCCATCAATGAGGAGAAGTTTGCCCGTGTAGAAGCTGAGTTACACCAGCT
  GCTGTTAGCTTCAGGCCGTGCCCATGTACTCTGGACCACAGACATCTATGGCCGGCTGGCTGCAGACTAT
  TTTAACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215593 representing NM_001024354
  Red=Cloning site Green=Tags(s)
  
  MHTLVFLSTRQVLQCQPAACQALPLLPRELFPLLFKVAFMDKKTAVLRELVHTWPFPLLSFQQLLQECAH
  CSRALLQERLSTESMQAVILGLTARIHAREAEAGTQPVCRKHTLRVLDMTGLLDDGVEQDPGTMSMWDCT
  AAVARTCIAQQQVGTAKPGLSPVPVEIRVDLRVNRASYTFLREALQSSVDSLLRLCCRDLRAEDLPMRNT
  VALLQLLDAGCLRRIDLRFNNLGLRGLSVIIPHVARFQHLASLRLHYVHGDSRQPSVDGEDNFRYFLAQM
  GRFICLRELSIGSSLLSGRLDQLLSTLQRPLESLELAFCALLPEDLRFLAQSSHAAHLKKLDLSGNDLSG
  NQLTPFQGLLQAVAATLLHLELTECQIADAQLLATLPTLTRCASLRYLGLYGNPLSMAGLKELLRDSVIQ
  AELRTVVHPFPVDCYEGLPWPPPASVLLEASINEEKFARVEAELHQLLLASGRAHVLWTTDIYGRLAADY
  FNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024354
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024354.1, NP_001019525.1
• RefSeq Size: 2173 bp
• RefSeq ORF: 1482 bp
• Locus ID: 500900
• UniProt ID: Q569B5
• Cytogenetics: 7q34
• MW: 54.9 kDa
• Gene Summary: Negatively regulates Toll-like receptor-mediated NF-kappa-B signaling by disrupting IKK core complex formation through interaction with IKBKB.[UniProtKB/Swiss-Prot Function]