Bckdhb (NM_019267) Rat Tagged ORF Clone

CAT#: RR215528

Bckdhb (Myc-DDK-tagged ORF) - Rat branched chain keto acid dehydrogenase E1, beta polypeptide (Bckdhb), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR215528

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bckdhb
• Synonyms: BCKDE1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215528 representing NM_019267
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTGGCTGCGCGCGCCGGTGGGCTGCTGCGGCTCGGGGCTGCAGGAGCGGAGCGGCGGCGGC
  GCGGATTGCGCTGCGCTGCGCTGGTGCAAGGCTTCTTGCAACCCGCTGTCGATGACGCGTCCCAGAAGCG
  GCGGGTGGCCCACTTCACTTTCCAGCCGGACCCAGAGTCCCTTCAGTATGGGCAAACTCAGAAAATGAAC
  CTCTTCCAGTCAATAACAAGTGCCTTGGATAACTCATTGGCCAAAGACCCTACTGCAGTAATATTTGGTG
  AGGACGTTGCCTTTGGTGGAGTCTTCCGATGTACTGTTGGCTTACGAGACAAATATGGAAAAGACAGAGT
  GTTTAACACCCCGTTGTGTGAACAAGGAATTGTTGGGTTTGGCATTGGAATCGCGGTCACTGGTGCTACA
  GCTATTGCGGAAATCCAGTTTGCCGACTATATTTTCCCTGCCTTTGATCAGATTGTCAACGAAGCTGCCA
  AGTATCGATACCGCTCTGGCGATCTTTTCAACTGTGGGAGCCTCACCATCCGGGCCCCTTGGGGCTGTGT
  GGGCCATGGGGCTCTTTACCATTCTCAGAGTCCTGAAGCCTTTTTTGCCCATTGCCCAGGAATCAAGGTG
  GTTATACCCAGAAGCCCTTTCCAGGCCAAGGGACTTCTGTTGTCATGCATAGAAGATAAAAATCCATGTA
  TATTTTTTGAACCTAAGATACTTTACCGGGCAGCAGTGGAACAGGTCCCAGTAGAACCCTACAAGATCCC
  CTTGTCTCAGGCCGAAGTCATCCAGGAGGGCAGCGATGTGACTCTGGTTGCCTGGGGCACTCAGGTTCAT
  GTCATCCGGGAGGTGGCTTCCATGGCCCAAGAAAAGCTTGGAGTGTCTTGTGAAGTCATCGATCTGCGGA
  CCATTGTGCCTTGGGATGTGGATACAGTTTGTAAGTCTGTGATCAAAACGGGGCGACTGTTGATCAGCCA
  CGAGGCGCCCTTGACAGGCGGCTTTGCCTCTGAGATCAGCTCCACGGTCCAGGAAGAATGTTTCTTGAAC
  CTAGAGGCTCCAATATCTAGAGTGTGTGGATATGACACCCCGTTTCCTCACATTTTTGAGCCCTTTTATA
  TCCCAGACAAATGGAAGTGCTATGATGCCCTTCGCAAGATGATCAACTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215528 representing NM_019267
  Red=Cloning site Green=Tags(s)
  
  MAAVAARAGGLLRLGAAGAERRRRGLRCAALVQGFLQPAVDDASQKRRVAHFTFQPDPESLQYGQTQKMN
  LFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGAT
  AIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKV
  VIPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAVEQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVH
  VIREVASMAQEKLGVSCEVIDLRTIVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLN
  LEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019267
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019267.1, NP_062140.1
• RefSeq Size: 1491 bp
• RefSeq ORF: 1173 bp
• Locus ID: 29711
• UniProt ID: P35738
• Cytogenetics: 8q31
• MW: 42.8 kDa
• Gene Summary: plays a role in the conversion of 3-methyl-2-oxobutanoate and lipoamide to S-(2-methylpropanoyl)dihydrolipoamide and CO2 [RGD, Feb 2006]