Dazap1 (NM_001025742) Rat Tagged ORF Clone

CAT#: RR215249

Dazap1 (Myc-DDK-tagged ORF) - Rat DAZ associated protein 1 (Dazap1), (10 ug)

Product Images

Circular map for RR215249

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dazap1
• Synonyms: MGC114376
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215249 representing NM_001025742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCGCGGGCGCCGACGAAATCGGGAAGCTCTTTGTGGGCGGCCTGGACTGGAGTACCACTCAAG
  AGACTCTGCGCAGCTACTTTTCCCAGTATGGTGAGGTTGTGGATTGTGTCATCATGAAAGATAAAACCAC
  CAACCAGTCTCGAGGCTTTGGATTTGTCAAGTTTAAAGACCCCAATTGTGTGGGGACAGTGCTGGCCAGC
  AGACCACACACCCTAGATGGCCGGAATATTGACCCGAAGCCATGCACACCTCGGGGGATGCAGCCAGAGC
  GAACGCGGCCCAAGGAAGGCTGGAAAGGACCCAGGAGCGACAGCAGCAAATCCAACAAGATCTTTGTTGG
  GGGCATTCCCCACAACTGCGGCGAGACGGAGCTCAGGGAGTACTTCAAGAAGTTTGGAGTGGTCACAGAG
  GTCGTTATGATCTATGATGCCGAGAAGCAGCGGCCTCGAGGTTTTGGATTTATTACTTTCGAGGACGAAC
  AATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAGGTTAAACGGGC
  TGAACCTCGGGACAGCAAAAACCAAGCACCAGGACAGCCAGGAGCCAGCCAGTGGGGCAGCCGCGTGGCG
  CCCAGTGCAGCCAACGGCTGGGCGGGCCAGCCCCCGCCAACGTGGCAACAAGGATATGGCCCACAAGGAA
  TGTGGGTGCCAGCAGGACAGGCCATTGGTGGCTATGGCCCACCCCCTGCGGGCAGAGGAGCCCCGCCCCC
  GCCCCCACCCTTCACCTCCTACATTGTGTCCACACCTCCTGGAGGCTTCCCCCCACCACAGGGCTTCCCA
  CAGGGCTACGGCGCTCCACCACAGTTCAGTTTTGGCTACGGTCCTCCGCCCCCACCCCCTGATCAGTTCG
  CCCCTCCAGGGGTCCCTCCTCCACCTGCCACCCCAGGGGCTGCCCCACTGGCCTTCCCACCACCTCCGTC
  TCAGGCTGCCCCAGACATGAGCAAACCCCCAACAGCCCAGCCGGACTTCCCCTATGGTCAATATGGTTAC
  GGGCAGGACTTGAGCGGCTTCGGCCAGGGCTTCTCAGACCCCAGCCAGCAGCCGCCCTCCTATGGGGGCC
  CCTCTGTGCCGGGCTCAGGGGGTCCCCCCGCTGGTGGCAGTGGCTTCGGACGTGGGCAGAACCACAACGT
  GCAGGGCTTCCATCCCTACAGGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215249 representing NM_001025742
  Red=Cloning site Green=Tags(s)
  
  MNSAGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLAS
  RPHTLDGRNIDPKPCTPRGMQPERTRPKEGWKGPRSDSSKSNKIFVGGIPHNCGETELREYFKKFGVVTE
  VVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKNQAPGQPGASQWGSRVA
  PSAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGFP
  QGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPATPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYGY
  GQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025742
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025742.1, NP_001020913.1
• RefSeq Size: 1574 bp
• RefSeq ORF: 1218 bp
• Locus ID: 362836
• Cytogenetics: 7q11
• MW: 43.1 kDa